Antimicrobial resistance of Escherichia coli isolates from outpatient urinary tract infections in women in six European countries including Russia

Ny, Sofia; Edquist, Petra; Dumpis, Uga; Gröndahl-Yli-Hannuksela, Kirsi; Hermes, Júlia; Kling, Anna-Maria; Klingeberg, Anja; Kozlov, Roman; Källman, Owe; Lis, Danuta O.; Pomorska-Wesołowska, Monika; Saule, Mära; Wisell, Karin Tegmark; Vuopio, Jaana; Palagin, Ivan

doi:10.1016/j.jgar.2018.11.004

Cited by 75 publications

(53 citation statements)

References 18 publications

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“…Nevertheless, resistance rates fluctuate by country. In comparison, the rates observed in this study for Cameroon are higher than those registered in 6 European countries including Russia [46], and lower than those from Nigeria [47]. The spectrum of antibiotics used in Cameroon for coli carrying the blaOxa, blaTEM, cat1, cat2, sul1, and sul2 genes, were distinguished by their association with each of the 3 virulent genes (eae, aaic, CVD432).…”

Section: Discussioncontrasting

confidence: 63%

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent <italic>Escherichia Coli</italic> from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Marbou

Jain

Samajpati

et al. 2020

Osong Public Health Res Perspect

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This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon. Methods: A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/ simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection. Results: There were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazoletrimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC. Conclusion: These findings suggested that measures should be taken to reduce the harm of E. coli to public health.

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Section: Discussioncontrasting

confidence: 63%

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent <italic>Escherichia Coli</italic> from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Marbou

Jain

Samajpati

et al. 2020

Osong Public Health Res Perspect

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“…A total of 150 samples were collected from patients suffering from urinary tract infection, both males and females. The freshly voided clean catch midstream urine samples were taken to the laboratory and proceed upon arrival as soon as possible to avoid contamination [21,22].…”

Section: Sample Size and Sample Collectionmentioning

confidence: 99%

Prevalence and Antibiogram of MDR E. coli Strains Isolated from UTI Patients—1-Year Retrospective Study at Nishtar Medical Hospital, Multan

Fatima

Rafiq

Iqbal

et al. 2020

SN Compr. Clin. Med.

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MDR uropathogens lead to high-level morbidity and mortality and pose a tremendous threat towards treatment failure and survival of UTI patients. This study was conducted to evaluate the demographic characteristics, antibiogram, and resistance pattern of Escherichia coli against antibiotics-a retrospective study at Nishtar Medical Hospital in Multan, from June 2016 to July 2017. A total of 150 clinical samples were collected and biochemically characterized by Berge's manual, and antimicrobial susceptibility testing was performed by using the Kirby-Bauer disc diffusion method and the results were interpreted according to Clinical and Laboratory Standards Institute standards. Data was analyzed by the XL-stat software. Results indicate that UTI was more prevalent in rural areas such as Alipur (28.6%) and Jalalpur (18%) and less common in urban areas like Muzaffargarh (2.66) and Multan (2%). A total of 150 patients were examined, of which 88 were females and 62 males in which more frequently found pathogens were E. coli (33%) and others Klebsiella pneumonia (18%), Proteus (10%), Staphylococcus saprophyticus (8.66%), Staphylococcus aureus (7.33%), Pseudomonas aeruginosa (6.66%), Enterobacter (6%), Candida (5.33%), and Citrobacter (4.66%). The antimicrobial susceptibility testing was carried out against E. coli strains that showed the highest overall resistance patterns of imipenem (IMI), Cip, Aug, Cfm, and CN, which were 80%, 72%, 68%, 60%, and 52%, respectively. MDR E. coli strains showed the highest resistance pattern towards IMI which put through alarming situation in Pakistan. Significant results showed that TZP, AK, and F were found sensitive in vitro against E. coli isolates of UTIs. Prevalence of MDR uropathogens near around Multan has been first time documented in this study with respect to possible risk factors associated with UTI. These alarming signs of MDR E. coli have been rarely addressed and warrant attention on urgent basis.

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“…Это в особенной степени относится к внебольничным инфекциям мочевых путей (ИМП), для которых терапия в большинстве случаев назначается эмпирически. В России, как и во многих странах мира, в последние годы отмечается резкое снижение чувствительности внебольничных уропатогенов [1][2][3][4]. Энтеробактерии, и прежде всего Escherichia coli, оставаясь основными бактериальными возбудителями ИМП [5,6], обладают высокой способностью к формированию вторичной резистентности к антибиотикам различных классов, что в современных условиях диктует необходимость мониторинга и анализа динамики устойчивости к АМП.…”

Section: Introductionunclassified

Antimicrobial resistance of pathogens causing community-acquired urinary tract infections in Russia: results of the multicenter study “DARMIS-2018”

Palagin

Сухорукова

Дехнич

et al. 2019

CMAC

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Цель. Определить in vitro активность антимикробных препаратов в отношении клинических изолятов, выделенных от пациентов с внебольничными инфекциями мочевых путей (ИМП) в различных регионах России в 2017-2018 гг. Материалы и методы. В анализ включено 1052 изолятов порядка Enterobacterales, выделенных в рамках многоцентрового, проспективного, эпидемиологического исследования динамики антибиотикорезистентности возбудителей внебольничных ИМП в различных субпопуляциях пациентов («ДАРМИС-2018») в Российской Федерации. Штаммы уропатогенов были получены от детей и взрослых обоего пола всех возрастных групп с острыми (и обострением хронических) внебольничными ИМП, включая беременных с бессимптомной бактериурией, в 32 центрах 24 городов России в 2017-2018 гг. Результаты. Энтеробактерии составили в общей сложности 90,6% от всех выделенных бактериальных возбудителей (90,6% в субпопуляции взрослых; 89,3% в субпопуляции беременных; 93,8% в субпопуляции детей и подростков). Наиболее частыми видами были Escherichia coli (71,3% в субпопуляции взрослых; 73,6% в субпопуляции беременных; 79,7% в субпопуляции детей и подростков) и Klebsiella pneumoniae (11,7% в субпопуляции взрослых; 10,4% в субпопуляции беременных; 7,8% в субпопуляции детей и подростков). Из пероральных препаратов максимальной активностью в отношении E. coli обладали фосфомицин (97,7% в субпопуляции взрослых; 95,9% в субпопуляции беременных; 99,0% в субпопуляции детей и подростков) и нитрофурантоин (98,1% в субпопуляции взрослых; 100% в субпопуляции беременных; 97,1% в субпопуляции детей и подростков). Из парентеральных препаратов наибольшей активностью обладали меропенем (из карбапенемов) и амикацин (99,4% и 97,9% в субпопуляции взрослых; 99,5% и 99,1% в субпопуляции беременных; 100% и 97,1% в субпопуляции детей и подростков до 18 лет соответственно). Наименьшую in vitro активность в отношении E. coli (менее 80% для каждой субпопуляции пациентов) продемонстрировали ампициллин, амоксициллин/клавуланат и триметоприм/сульфаметоксазол. Чувствительность E. coli к ципрофлоксацину составила 60,6% в субпопуляции взрослых, 80,0% в субпопуляции беременных и 80,4% в субпопуляции детей и подростков. Частота продукции бета-лактамаз расширенного спектра по результатам фенотипических тестов составила 27,0% в субпопуляции взрослых, 8,6% в субпопуляции беременных и 23,5% в субпопуляции детей и подростков. Выводы. Результаты исследования свидетельствуют о росте резистентности к большинству антимикробных препаратов среди внебольничных штаммов Enterobacterales, в частности E. coli, в России.

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Antimicrobial resistance of Escherichia coli isolates from outpatient urinary tract infections in women in six European countries including Russia

Cited by 75 publications

References 18 publications

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent <italic>Escherichia Coli</italic> from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent <italic>Escherichia Coli</italic> from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Prevalence and Antibiogram of MDR E. coli Strains Isolated from UTI Patients—1-Year Retrospective Study at Nishtar Medical Hospital, Multan

Antimicrobial resistance of pathogens causing community-acquired urinary tract infections in Russia: results of the multicenter study “DARMIS-2018”

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Antimicrobial resistance of Escherichia coli isolates from outpatient urinary tract infections in women in six European countries including Russia

Cited by 75 publications

References 18 publications

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent &lt;italic&gt;Escherichia Coli&lt;/italic&gt; from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent &lt;italic&gt;Escherichia Coli&lt;/italic&gt; from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Prevalence and Antibiogram of MDR E. coli Strains Isolated from UTI Patients—1-Year Retrospective Study at Nishtar Medical Hospital, Multan

Antimicrobial resistance of pathogens causing community-acquired urinary tract infections in Russia: results of the multicenter study “DARMIS-2018”

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Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent <italic>Escherichia Coli</italic> from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent <italic>Escherichia Coli</italic> from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon