Antimicrobial resistance in Enterobacteriaceae in Brooklyn, NY: epidemiology and relation to antibiotic usage patterns

Saurina, Guillermo; Quale, John; Manikal, Vivek M.; Oydna, Elise; Landman, David

doi:10.1093/jac/45.6.895

Cited by 96 publications

(56 citation statements)

References 7 publications

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“…We observed antimicrobial resistance in all strains of Entrobacteriaceae when tested against several classes of antibiotics including kanamycin, methicillin, penicillin G, cefotaxime, sulphafurazole, rifampicin and chloramphenicol (Table 2). These observations are in agreement with Saurina et al (2000) who studied on epidemiology and antibiotics usage patterns in human medicine in USA. Indiscriminate use of antibiotics and their contamination to the environment may have increased the selective pressure towards the development of multi drug resistant strains in the family to antibiotics (Narins, 2003).…”

Section: Discussionsupporting

confidence: 92%

Qualitative Assessment of Livestock Faeces and Sewage for Antibiotic Resistant Strains of E. coli in Roma, Lesotho

Mekbib¹,

Marole²,

Asita³

2013

GJBS

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Sewage water (SW) and fresh livestock faeces: Chicken (ChNF), Sheep (SF), and Cattle (CF) were taken randomly from the farm of the National University of Lesotho aseptically during the 2012 academic year. Twenty ml of sewage water or 20 grams of fresh animal faeces was dissolved in 80 ml of sterilized Ringer's solution and serially diluted. A 0.1 ml of sample suspension at 10 -4 dilution was spread plated on Nutrient Agar (NA) and Eosine Methyl Blue (EMB) and plates were incubated for 24-48h at 24 and 37°C, respectively. A disc diffusion method was used to test the reaction of selected strains of Enterobacteriaceae to nine antibiotics. In total, 24 strains under Enterobacteriaceae were isolated from the respective sources and the number of each that were Escherichia coli, as confirmed by the biochemical tests were: SW (7 and 3); ChNF (5 and 3); SF (8 and 6) and CF (4 and 2). Thirteen E. coli strains fermented lactose and were Catalase positive, while only one E. coli strain from SW was non-lactose fermenter and Catalase negative.

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Section: Discussionsupporting

confidence: 92%

Qualitative Assessment of Livestock Faeces and Sewage for Antibiotic Resistant Strains of E. coli in Roma, Lesotho

Mekbib¹,

Marole²,

Asita³

2013

GJBS

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“…En adultos y niños menores de 5 años está indicada la ciprofloxacina como droga de primera elección, y la azitromicina está indicada para los niños que no requieren hospitalización [8,30].…”

Section: Disusiónunclassified

Sensibilidad antimicrobiana entre los serogrupos de <em>Shigellae</em> aislados en la ciudad de Quito-Ecuador

Granda

Alcocer

2015

Av. Cienc. Ing. (Quito)

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The pathotype Shigellae includes bacillus from the species S. flexneri, S. sonnei, S. boydii and S. dysenteriae which are causatives of the disease known as bacillary dysentery or shigellosis. The pathogenicity of the genus is characterized by the presence of high antibiotic resistance. The aim of this study was to identify the species and antimicrobial susceptibility in clinical isolates of Shigellae. We analyzed 79 isolates obtained from Zurita & Zurita Laboratorios and Vozandes Hospital in the city of Quito. Three species were obtained by serotyping, using polyclonal antisera: S. flexneri (n=50; 63,29 %), S. sonnei (n=23; 29,11 %), and S. boydii (n=6; 7,59 %). The antimicrobial susceptibility was analyzed using the Kirby Bauer method and recommendations from the "Clinical and Laboratory Standards Institute" (CLSI). Most of the isolates showed resistance to tetracycline (n=76; 96,20 %), ampicillin (n=75; 94,94 %), trimethoprim/sulfamethoxazole (n= 68; 86,08 %) and chloramphenicol (n=67; 84,81 %). The results showed the incidence of multidrug resistance to antibiotics commonly used for treating shigellosis, and the presence of three species of Shigellae.Keywords. Antibiotics, bacillary dysentery, Shigellae. ResumenEl patotipo Shigellae comprende bacilos de las especies S. flexneri, S. sonnei, S. boydii y S. dysenteriae, causantes de la enfermedad conocida como disentería bacilar o shigelosis. La patogenicidad del género se caracteriza por la presencia de una alta multirresistencia a antibióticos. El objetivo de este estudio fue identificar las especies y la sensibilidad a antimicrobianos en aislados clínicos de Shigellae. Se analizaron 79 aislados obtenidos de Zurita & Zurita Laboratorios y del Hospital Vozandes en la ciudad de Quito. Mediante serotipaje, con el uso de antisueros policlonales, se obtuvieron 3 especies: S. flexneri (n=50; 63,29 %), S. sonnei (n= 23; 29,11 %), y S. boydii (n= 6; 7,59 %). La sensibilidad antimicrobiana se analizó siguiendo el método de Kirby-Bauer y las recomendaciones del "Clinical and Laboratory Standards Institute" (CLSI). La mayoría de las cepas mostraron resistencia a tetraciclina (n=76; 96,20 %), ampicilina (n=75; 94,94 %), trimetoprim/sulfametoxazol (n=68; 86,08 %) y cloranfenicol (n=67; 84,81 %). Los resultados obtenidos demostraron la incidencia de multirresistencia a antibióticos comúnmente utilizados para el tratamiento de shigelosis, y la presencia de 3 especies de Shigellae.Palabras Clave. Antibióticos, disentería bacilar, Shigellae. IntroducciónEl patotipo Shigellae comprende bacilos Gram negativos de 0,3 a 1 µm diámetro y de 1 a 6 µm de longitud, no móviles, no formadores de esporas, anaerobios facultativos, pertenecientes a la familia Enterobacteriaceae y causantes de la enfermedad conocida como shigelosis o disentería bacilar [1]. Su identificación se fundamenta en características bioquímicas y antigénicas, en base a ellas se describen cuatro especies: Shigella dysenteriae, serogrupo A; Shigella flexneri, serogrupo B; Shigella boydii, serogrupo C; y, Shige...

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“…Non-TEM-derived ESBLs such as VEB-1 (Kim et al, 2004), CTX-M b-lactamases (CTX-M-1, CTX-M-2, CTX-M-13 and CTX-M-31) (Ho et al, 2005;Karapavlidou et al, 2005) and plasmid-borne AmpC blactamases (CMY-2 and CMY-4) (Verdet et al, 1998;Park et al, 2006) have also been reported infrequently. The prevalence of ESBL-positive strains has been reported at 6.9 % in France , 7.0 % in Argentina (Quinteros et al, 2003), 8.8 % in Italy (Luzzaro et al, 2001) and 9.5 % in the US (Saurina et al, 2000). An outbreak of ESBL-producing P. mirabilis has been reported as a nosocomial infection caused by clonal spread (Nagano et al, 2003;Kim et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Nakano

Abe

et al. 2012

Journal of Medical Microbiology

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Proteus mirabilis is a common cause of urinary tract infection. Wild-type P. mirabilis strains are usually susceptible to penicillins and cephalosporins, but occurrences of P. mirabilis producing extended-spectrum b-lactamases (ESBLs) have been recently reported. Here, we surveyed the prevalence of cefotaxime resistance among P. mirabilis strains at seven different hospitals in Kanagawa Prefecture, Japan, and investigated their molecular epidemiology to explain the mechanism of their spread. The prevalence of cefotaxime resistance among P. mirabilis increased annually, from 10.1 % in 1998 to 23.1 % in 2003, and increased drastically in 2004, exceeding 40 %. We collected 105 consecutive and non-duplicate cefotaxime-resistant P. mirabilis isolates (MIC 16 to .256 mg ml "1 ) from these hospitals from June 2004 to May 2005 and characterized their profile. PCR and sequence analysis revealed that all resistant strains produced exclusively CTX-M-2 b-lactamase. PFGE analysis identified 47 banding patterns with 83 % or greater similarity. These results indicated that a regional outbreak of P. mirabilis producing CTX-M-2 blactamase has occurred in Japan and suggest that the epidemic spread occurred within and across hospitals and communities by extended clonal strains. Plasmid analysis revealed that 44.8 % of plasmids harboured by bla CTX-M-2 isolates had common profiles, encoding ISEcp1, IS26 and Int1, and belonged to incompatibility group T. Spread of the resistant isolates in Japan resulted from dissemination of narrow-host-range plasmids of the IncT group encoding bla CTX-M-2 . These findings indicate the rapidly developing problem of treating the species to prevent dissemination of ESBL producers.

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Antimicrobial resistance in Enterobacteriaceae in Brooklyn, NY: epidemiology and relation to antibiotic usage patterns

Cited by 96 publications

References 7 publications

Qualitative Assessment of Livestock Faeces and Sewage for Antibiotic Resistant Strains of E. coli in Roma, Lesotho

Qualitative Assessment of Livestock Faeces and Sewage for Antibiotic Resistant Strains of E. coli in Roma, Lesotho

Sensibilidad antimicrobiana entre los serogrupos de <em>Shigellae</em> aislados en la ciudad de Quito-Ecuador

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

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