Sensibilidad antimicrobiana entre los serogrupos de <em>Shigellae</em> aislados en la ciudad de Quito-Ecuador

Abstract

The pathotype Shigellae includes bacillus from the species S. flexneri, S. sonnei, S. boydii and S. dysenteriae which are causatives of the disease known as bacillary dysentery or shigellosis. The pathogenicity of the genus is characterized by the presence of high antibiotic resistance. The aim of this study was to identify the species and antimicrobial susceptibility in clinical isolates of Shigellae. We analyzed 79 isolates obtained from Zurita & Zurita Laboratorios and Vozandes Hospital in the city of Quito. Three species were obtained by serotyping, using polyclonal antisera: S. flexneri (n=50; 63,29 %), S. sonnei (n=23; 29,11 %), and S. boydii (n=6; 7,59 %). The antimicrobial susceptibility was analyzed using the Kirby Bauer method and recommendations from the "Clinical and Laboratory Standards Institute" (CLSI). Most of the isolates showed resistance to tetracycline (n=76; 96,20 %), ampicillin (n=75; 94,94 %), trimethoprim/sulfamethoxazole (n= 68; 86,08 %) and chloramphenicol (n=67; 84,81 %). The results showed the incidence of multidrug resistance to antibiotics commonly used for treating shigellosis, and the presence of three species of Shigellae.Keywords. Antibiotics, bacillary dysentery, Shigellae. ResumenEl patotipo Shigellae comprende bacilos de las especies S. flexneri, S. sonnei, S. boydii y S. dysenteriae, causantes de la enfermedad conocida como disentería bacilar o shigelosis. La patogenicidad del género se caracteriza por la presencia de una alta multirresistencia a antibióticos. El objetivo de este estudio fue identificar las especies y la sensibilidad a antimicrobianos en aislados clínicos de Shigellae. Se analizaron 79 aislados obtenidos de Zurita & Zurita Laboratorios y del Hospital Vozandes en la ciudad de Quito. Mediante serotipaje, con el uso de antisueros policlonales, se obtuvieron 3 especies: S. flexneri (n=50; 63,29 %), S. sonnei (n= 23; 29,11 %), y S. boydii (n= 6; 7,59 %). La sensibilidad antimicrobiana se analizó siguiendo el método de Kirby-Bauer y las recomendaciones del "Clinical and Laboratory Standards Institute" (CLSI). La mayoría de las cepas mostraron resistencia a tetraciclina (n=76; 96,20 %), ampicilina (n=75; 94,94 %), trimetoprim/sulfametoxazol (n=68; 86,08 %) y cloranfenicol (n=67; 84,81 %). Los resultados obtenidos demostraron la incidencia de multirresistencia a antibióticos comúnmente utilizados para el tratamiento de shigelosis, y la presencia de 3 especies de Shigellae.Palabras Clave. Antibióticos, disentería bacilar, Shigellae. IntroducciónEl patotipo Shigellae comprende bacilos Gram negativos de 0,3 a 1 µm diámetro y de 1 a 6 µm de longitud, no móviles, no formadores de esporas, anaerobios facultativos, pertenecientes a la familia Enterobacteriaceae y causantes de la enfermedad conocida como shigelosis o disentería bacilar [1]. Su identificación se fundamenta en características bioquímicas y antigénicas, en base a ellas se describen cuatro especies: Shigella dysenteriae, serogrupo A; Shigella flexneri, serogrupo B; Shigella boydii, serogrupo C; y, Shige...

Prevalence and antimicrobial profile of Shigella causing acute diarrheal disease in pediatrics in Posadas, Misiones

“…The high resistance detected for ampicillin and trimethoprim-sulfamethoxazole and almost nil for ciprofloxacin is similar to surveillance results reported by the WHONET-SIREVA II Network (2020) for the period 2010-2019 as well as with different studies from Argentina [11,23], Ecuador [20] and Peru [21].…”

“…When analysing our results, we observed that the same pattern of species shown by developing countries is maintained, as reported for other Latin American countries [19][20][21] and other Argentine provinces [11,17,[22][23][24][25] in the years 2020, 2019, 2017, 2012 and 2007 respectively, where S. flexneri predominated with a percentage between 60 and 70%, followed by S. sonnei.…”

Genetic and Genomic Diversity in a Tarwi (Lupinus mutabilis Sweet) Germplasm Collection and Adaptability to Mediterranean Climate Conditions

“…In spite of their high alkaloid content [7,8], tarwi seeds have high nutritional value, containing up to 53% protein and 24% lipids [9]. The nutritional attributes found in tarwi are supposedly better than those in soybeans [10] and for this reason it is called Andean soybean [11]. Tarwi protein is rich in globulins (43%-45%) and albumins (8%-9%) and the oil has high quality and does not require industrial removal of the linolenic acid like in soybean [12,13].…”