Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

Goualié, Bernadette; Akpa, Eric Essoh; Kakou-Ngazoa, Solange; Ouattara, Honoré G.; Niamké, Sebastien; Dosso, Mireille

doi:10.3855/jidc.11355

Cited by 10 publications

(19 citation statements)

References 37 publications

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“…Our study revealed a significant prevalence of Campylobacter spp in poultry feces (67.96%) and in retail poultry carcasses (50%). These frequencies are consistent with those reported in previous studies conducted in Sri Lanka (Kottawatta et al., ) and in Ivory Coast (Goualié et al., ). Conversely, a higher prevalence of Campylobacter was observed in poultry carcasses in Japan (Furukawa et al., ), in Maryland (Cui, Ge, Zheng, & Meng, ), and in poultry feces in the Netherlands (Schets et al., ).…”

Section: Discussionsupporting

confidence: 93%

“…jejuni was the most prevalent with 70% rate in poultry feces and 100% in carcasses. Similar results were also reported in studies conducted in Ivory Coast (Goualié et al., ) and in Brazil (Perdoncini et al., ). In contrast, in Asia and Thailand, two studies revealed a higher percentage of C. coli than C. jejuni (Kottawatta et al., ; Padungtod & Kaneene, ).…”

Section: Discussionsupporting

confidence: 90%

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Salmonella spp. and Campylobacter spp. in poultry feces and carcasses in Ouagadougou, Burkina Faso

Kagambèga

Thibodeau

Trinetta

et al. 2018

Food Science & Nutrition

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The importance of Salmonella and Campylobacter as foodborne pathogens is well recognized worldwide. Poultry and poultry products are commonly considered as the major vehicles of Salmonella and Campylobacter infection in humans. The aim of this study was to investigate the hygienic status of poultry facilities and determine the prevalence of Salmonella and Campylobacter in slaughtered poultry feces and carcasses in four different markets in Ouagadougou, capital city of Burkina Faso. A total of 103 poultry feces and 20 carcasses were analyzed using microbiological standard methods. Among the 103 fecal samples, 70 were positive for Campylobacter ssp (67.96%) and 54 for Salmonella ssp (52.42%). The hippurate hydrolysis test revealed that among the 70 Campylobacter strains isolated from feces, 49 were C. jejuni (70%) and 21 were C. coli (30%). From the 20 carcasses analyzed, 18 were contaminated with Salmonella (90%) and 10 with Campylobacter ssp (50%). Among the 10 Campylobacter ssp samples isolated from poultry carcasses, all were identified as C. jejuni using the API CAMPY system and the hippurate hydrolysis test. The assessment of markets hygienic practices for production, transportation, display, and vending of meat revealed unhygienic conditions. To complete the observation of unhygienic practices, we have sampled chicken‐washing solution from the study sites and microbiological analysis of these samples revealed the presence of Salmonella spp in 100% of the samples. This study highlighted that poultry products on sale in Ouagadougou are highly contaminated with Salmonella and Campylobacter. To the best of our knowledge, this is the first report describing Campylobacter presence in the poultry industry of Burkina Faso. Our findings might help to better understand the epidemiology of Salmonella and Campylobacter.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Salmonella spp. and Campylobacter spp. in poultry feces and carcasses in Ouagadougou, Burkina Faso

Kagambèga

Thibodeau

Trinetta

et al. 2018

Food Science & Nutrition

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“…Some studies using AST performed worldwide corroborated the present work and also showed that C. jejuni strains are resistant to ciprofloxacin, tetracycline, and erythromycin. [25][26][27][28][29] Duarte et al 27 studied 89 C. jejuni strains isolated from humans, animals, and food and observed resistance to ciprofloxacin, tetracycline, and erythromycin in 82, 59, and 6 strains, respectively. Fifteen of the 39 C. jejuni strains isolated from poultry in Côte d'Ivoire were resistant to ciprofloxacin, and seven strains were resistant to erythromycin.…”

Section: Discussionmentioning

confidence: 99%

“…Fifteen of the 39 C. jejuni strains isolated from poultry in Côte d'Ivoire were resistant to ciprofloxacin, and seven strains were resistant to erythromycin. 25 A study performed in three regions of Peru evaluated the ciprofloxacin resistance of C. jejuni strains isolated from humans in two different periods. These authors observed a significant increase in the resistant strains in all the regions, including 72.6% to 82.8% in Cusco, 24.1% to 48.9% in Iquitos, and 73.1% to 89.8% in Lima.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Profiles and Phylogenetic Analysis of Campylobacter jejuni Strains Isolated in Brazil by Whole Genome Sequencing

Frazão

Cao

Medeiros

et al. 2020

Microbial Drug Resistance

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The objectives of this work were to use whole genome sequencing (WGS) to determine the antimicrobial resistance genotypes of 116 Campylobacter jejuni strains isolated in Brazil and to compare it with the results obtained by antimicrobial susceptibility testing (AST). In addition, WGS was used to uncover the phylogenetic relationship among those strains. Results: By AST, the C. jejuni strains resistant to ciprofloxacin, tetracycline, doxycycline, and erythromycin were 51 (44%), 41 (35.3%), 41 (35.3%), and 6 (5.2%), respectively. By WGS, the genes aph(3¢)III, aadE, bla OXA-449 , bla OXA-184 , bla OXA-61 , and tet(O) were detected in 6 (5.2%), 3 (2.6%), 1 (0.9%), 10 (8.6%), 55 (47.4%), and 44 (38%) strains, respectively. Fifty-four (46.6%) strains showed the mutation T86I in the gyrA gene, and four (3.4%) strains presented the mutation A2075G in the 23S rRNA gene. The correlation between AST and WGS was 100% for ciprofloxacin, 97.5% for tetracyclines, and 66.7% for erythromycin. The whole genome single nucleotide polymorphism (SNP) tree clustered the C. jejuni strains into two clades comprising strains that were highly related from different sources, places, and years. Conclusion: The high rates of C. jejuni strains resistant to ciprofloxacin and tetracyclines are of concern and may represent a public health problem. WGS has a potential to be a powerful tool for the prediction of resistance of antibiotics used to treat campylobacteriosis. The results obtained by whole genome SNP analysis suggested the potential for transmission between clinical and nonclinical sources and between human and animal sources over the course of 20 years in Brazil.

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“…Bacterial infections are mostly involved in causing diseases in sub-Saharan countries. Goualié et al (2019) reported the high incidence of multidrug resistant bacteria in chickens in Cote d'Ivoire. This problem is emergent because of the misuse of drugs and the free accessibility to drugs in human or in animal trials.…”

Section: Introductionmentioning

confidence: 99%

First novel phages from rodents with lytic activity on clinical Enterobacteriaceae strains: Initiation for phage therapy in West Africa

Solange¹,

Addablah²,

Katerina³

et al. 2020

Afr. J. Microbiol. Res.

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Bacteriophages (phages) are viruses that infect bacteria. The emergence of resistant bacteria has been reported worldwide. The use of phages to treat bacterial infection or to reduce bacteria load has become a new area of interest. The viral colonization of rodents represents an important source for a specific biotope. The objective of this study is to characterize the effect of novel phages isolated from rodents (Mastomys) using clinical Enterobacteria strains. Three novel phages were isolated from the intestinal tract of rodents. The phages belong to Myoviridae family. By using three Random Polymerase Chain Reactions (PCR), genomic characterization of the phages shows a high diversity that reveals several DNA bands. 3 virulent phages exhibited different patterns, indicating that the rodents' phages are genetically unique and could be distinguished by typing-PCR. The virulence spectrum of the phages shows phages Mα3 and Mα7 have 50% (7/15) lytic activity on enteropathogenic and enterohemorrhagic Escherichia coli strains while phage Mα11 has 26.6% (4/15) lytic activity. These phages have no lytic activity on Salmonella strains. This study is the first to isolate novel phages from rodents with lytic activity. The candidate rodents' phages (Mα3, Mα7, Mα11) can be used to control virulent E. coli in West Africa.

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Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

Cited by 10 publications

References 37 publications

Salmonella spp. and Campylobacter spp. in poultry feces and carcasses in Ouagadougou, Burkina Faso

Salmonella spp. and Campylobacter spp. in poultry feces and carcasses in Ouagadougou, Burkina Faso

Antimicrobial Resistance Profiles and Phylogenetic Analysis of Campylobacter jejuni Strains Isolated in Brazil by Whole Genome Sequencing

First novel phages from rodents with lytic activity on clinical Enterobacteriaceae strains: Initiation for phage therapy in West Africa

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