Antimicrobial resistance and population structure of Staphylococcus aureus recovered from pigs farms

Peeters, Laura; Argudín, M. Ángeles; Azadikhah, Sonya; Butaye, Patrick

doi:10.1016/j.vetmic.2015.08.018

Cited by 57 publications

(53 citation statements)

References 21 publications

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“…To provide a more extensive representation of European strains, 18 isolates originating from horses, pigs, poultry and rats previously studied (Jamrozy et al, 2012; Nemeghaire et al, 2014; Peeters et al, 2015) were supplied by the Veterinary and Agrochemical Research center (Brussels, Belgium) and the University of Ghent (Ghent, Belgium). The remaining CC398 strains (20 MSSA and 38 MRSA) were from whole genome sequences deposited in the NCBI database (http://www.ncbi.nlm.nih.gov/) from Price et al (2012), these included European and non-European isolates.…”

Section: Methodsmentioning

confidence: 99%

“…Antimicrobial susceptibility testing for the 7 PHE isolates was performed at PHE and AFB1, by agar dilution method as described Andrews (2001) and disk diffusion method in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines (CLSI, 2013a,b), respectively. Antimicrobial susceptibility data from MIC testing was available for 16 Belgian isolates for β-lactams, tetracycline, clindamycin, trimethoprim, aminoglycoside, erythromycin, chloramphenicol, and ciprofloxacin (Jamrozy et al, 2012; Nemeghaire et al, 2014; Peeters et al, 2015). …”

Section: Methodsmentioning

confidence: 99%

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Livestock-Associated Methicillin Resistant Staphylococcus aureus (LA-MRSA) Clonal Complex (CC) 398 Isolated from UK Animals belong to European Lineages

et al. 2016

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In recent years, there has been an increase in the number of livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 recovered from S. aureus isolated animals in the UK. To determine possible origins of 12 LA-MRSA CC398 isolates collected after screening more than a thousand S. aureus animal isolates from the UK between 2013 and 2015, whole genome sequences (WGS) of CC398 European, including UK, and non-European isolates from diverse animal hosts were compared. Phylogenetic reconstruction applied to WGS data to assess genetic relatedness of all 89 isolates, clustered the 12 UK CC398 LA-MRSA within the European sub-lineages, although on different nodes; implicating multiple independent incursions into the UK, as opposed to a single introduction followed by clonal expansion. Three UK isolates from healthy pigs and one from turkey clustered within the cassette chromosome recombinases ccr C S. aureus protein A (spa)-type t011 European sub-lineage and three UK isolates from horses within the ccrA2B2 t011 European sub-lineage. The remaining UK isolates, mostly from pigs, clustered within the t034 European lineage. Presence of virulence, antimicrobial (AMR), heavy metal (HMR), and disinfectant (DR) resistance genes were determined using an in-house pipeline. Most, including UK isolates, harbored resistance genes to ≥3 antimicrobial classes in addition to β-lactams. HMR genes were detected in most European ccrC positive isolates, with >80% harboring czrC, encoding zinc and cadmium resistance; in contrast ~60% ccrC isolates within non-European lineages and 6% ccrA2B2 isolates showed this characteristic. The UK turkey MRSA isolate did not harbor φAVβ avian prophage genes (SAAV_2008 and SAAV_2009) present in US MSSA isolates from turkey and pigs. Absence of some of the major human-associated MRSA toxigenic and virulence genes in the UK LA-MRSA animal isolates was not unexpected. Therefore, we can conclude that the 12 UK LA-MRSA isolates collected in the past 2 years most likely represent separate incursions into the UK from other European countries. The presence of zinc and cadmium resistance in all nine food animal isolates (pig and poultry), which was absent from the 3 horse isolates may suggest heavy metal use/exposure has a possible role in selection of some MRSA.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Livestock-Associated Methicillin Resistant Staphylococcus aureus (LA-MRSA) Clonal Complex (CC) 398 Isolated from UK Animals belong to European Lineages

et al. 2016

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“…Most of the animal isolates are negative for the pvl gene, but Belgian MRSA ST80-IV isolates from healthy pigs were positive for the pvl gene and corresponded to the communityacquired CA-MRSA ST80-IV European clone [121]. One Belgian pig CC80 strain contained an exfoliatin (etd), an epidermal cell diferentiation inhibitor (edinB), and staphylococcal exotoxin-like protein (setC) and IEC genes (isaB, isdA, hysA1, hysA2) [121].…”

Section: Antibiotic Resistance and Enterotoxin Genes In La-mrsa Cc398mentioning

confidence: 99%

“…One Belgian pig CC80 strain contained an exfoliatin (etd), an epidermal cell diferentiation inhibitor (edinB), and staphylococcal exotoxin-like protein (setC) and IEC genes (isaB, isdA, hysA1, hysA2) [121]. A comparison of the pvl-positive MRSA isolates (ST8-t008-IVa) from American pig and pet animals indicated the presence of common virulence proiles (lukSF-PV, clfA, clfB, fnbA, and sek) except for the fnbB gene of a canine isolate [118].…”

Section: Antibiotic Resistance and Enterotoxin Genes In La-mrsa Cc398mentioning

confidence: 99%

“…Although some studies suggested that the MRSA present in catle is bovine-speciic, most of the reports indicated that MRSA found in catle were derived from humans [89,92,115,116]. Bovine, porcine, canine, feline, and equine MRSA isolates containing the pvl gene and other virulence factors, such as chp, scn, seb, sek, and seq, toxic shock syndrome toxin 1 (tsst1 or tst) gene [91,117], hemolysin, protease, superantigen-like protein, capsule, and bioilm-associated genes [118][119][120][121], may pose a potential threat to public health. …”

Section: Introductionmentioning

confidence: 99%

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Methicillin-Resistant Staphylococcus aureus (MRSA) in Food- Producing and Companion Animals and Food Products

Chon¹,

Sung²,

Khan³

2017

Frontiers in Staphylococcus Aureus

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Methicillin-resistant Staphylococcus aureus (MRSA) has become a growing concern in companion and food-producing animals. The presence of multidrug-resistance with a wide range of extracellular enterotoxin genes, virulence factors, and Panton-Valentine leukocidin (pvl) cytotoxin genes confer life-threatening traits on MRSA and makes them highly pathogenic and diicult to treat. Clonal complex 398 (CC398), a predominant clonal lineage of livestock-associated-MRSA in domestic animals and retail meat, is capable of infecting humans. In order to monitor and prevent MRSA contamination, it is critical to understand its source and transmission dynamics. In this review, we describe MRSA in food-producing animals (pig, catle, chicken), horses, pet animals (dogs, cats), and food products (pork, beef, chicken, milk, and ish).

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Occupational Exposure to Biological Agents

Santos

Ramos

Vaz-Velho

et al. 2020

Advances in Intelligent Systems and Computing

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Antimicrobial resistance and population structure of Staphylococcus aureus recovered from pigs farms

Cited by 57 publications

References 21 publications

Livestock-Associated Methicillin Resistant Staphylococcus aureus (LA-MRSA) Clonal Complex (CC) 398 Isolated from UK Animals belong to European Lineages

Livestock-Associated Methicillin Resistant Staphylococcus aureus (LA-MRSA) Clonal Complex (CC) 398 Isolated from UK Animals belong to European Lineages

Methicillin-Resistant Staphylococcus aureus (MRSA) in Food- Producing and Companion Animals and Food Products

Occupational Exposure to Biological Agents

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