Antimicrobial Resistance and Molecular Characterization of Extended-Spectrum β-Lactamase-Producing<i>Escherichia coli</i>Isolated from Ducks in South Korea

Na, Seok Hyeon; Moon, Dong Chan; Choi, Myeong-Ja; Oh, Sun-Joo; Jung, Dae-Young; Sung, Eun Ji; Kang, Hee Young; Hyun, Bang-Hun; Lim, Suk-Kyung

doi:10.1089/fpd.2019.2644

Cited by 21 publications

(25 citation statements)

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“…Third-generation cephalosporin-resistant isolates are often resistant to multiple antimicrobials and are considered a potential threat to animal and human health [ 44 ]. The ceftiofur resistant rate in this study was slightly higher than previous reports in Korea (12%) [ 45 ] and the US (7%) [ 46 ]. Nevertheless, it was lower than Lee et al (22%) [ 47 ] and Zhang et al (47%) [ 33 ] in Korea and China, respectively.…”

Section: Discussioncontrasting

confidence: 83%

Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

et al. 2020

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We aimed to identify and characterize extended-spectrum β-lactamase (ESBL)-and/or plasmid-mediated AmpC β-lactamase (pAmpC)-producing Escherichia coli isolated from healthy broiler chickens slaughtered for human consumption in Korea. A total of 332 E. coli isolates were identified from 339 cloacal swabs in 2019. More than 90% of the isolates were resistant to multiple antimicrobials. ESBL/pAmpC-production was noted in 14% (46/332) of the isolates. Six of the CTX-M-β-lactamase-producing isolates were found to co-harbor at least one plasmid-mediated quinolone resistance gene. We observed the co-existence of blaCMY-2 and mcr-1 genes in the same isolate for the first time in Korea. Phylogenetic analysis demonstrated that the majority of blaCMY-2-carrying isolates belonged to subgroup D. Conjugation confirmed the transferability of blaCTX-M and blaCMY-2 genes, as well as non-β-lactam resistance traits from 60.9% (28/46) of the ESBL/pAmpC-producing isolates to a recipient E. coli J53. The ISECP, IS903, and orf477 elements were detected in the upstream or downstream regions. The blaCTX-M and blaCMY-2 genes mainly belonged to the IncI1, IncHI2, and/or IncFII plasmids. Additionally, the majority of ESBL/pAmpC-producing isolates exhibited heterogeneous PFGE profiles. This study showed that healthy chickens act as reservoirs of ESBL/pAmpC-producing E. coli that can potentially be transmitted to humans.

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Section: Discussioncontrasting

confidence: 83%

Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

et al. 2020

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“…In this study, 28 of 29 ESBL-EC isolates had CTX-M genotypes, just as CTX-M ESBLs have increased in occurrence worldwide since the 2000s [60]. Although genotypes of our ESBL-ECs were diverse, CTX-M group 1 (bla CTX-M-1 , bla CTX-M-3 , bla CTX-M-15 , bla CTX-M-55 ) and CTX-M group 9 (bla CTX-M-14 , bla CTX-M-27 , bla CTX-M-65 ) were prevalent types, which was consistent with the findings of previous studies in Korea [25][26][27][28]30,32,52,61,62]. The most predominant CTX-M genotype was CTX-M-55, which is a variant of globally emerging CTX-M-15, and it varies by a single amino acid substitution (Ala77→Val) that contributes to enhancing the enzymatic activity [60,63].…”

Section: Discussionsupporting

confidence: 91%

Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Retail Meats in Korea

Kim

et al. 2020

Microorganisms

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The spread of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) has posed a critical health risk to both humans and animals, because resistance to beta-lactam antibiotics makes treatment for commonly infectious diseases more complicated. In this study, we report the prevalence and genetic characteristics of ESBL-ECs isolated from retail meat samples in Korea. A total of 1205 E. coli strains were isolated from 3234 raw meat samples, purchased from nationwide retail stores between 2015 and 2018. Antimicrobial susceptibility testing was performed for all isolates by a broth microdilution method, and the ESBL phenotype was determined according to the Clinical and Laboratory Standards Institute (CLSI) confirmatory method. All ESBL-EC isolates (n = 29) were subjected to whole-genome sequencing (WGS). The antimicrobial resistance genes, plasmid incompatibility types, E. coli phylogroups, and phylogenetic relations were investigated based on the WGS data. The prevalence of ESBL-ECs in chicken was significantly higher than that in other meat samples. The results in this study demonstrate that clonally diverse ESBL-ECs with a multidrug resistance phenotype were distributed nationwide, although their prevalence from retail meat was 0.9%. The dissemination of ESBL-ECs from retail meat poses a potential risk to consumers and food-handlers, suggesting that the continuous surveillance of ESBL-ECs in retail meat should be conducted at the national level.

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“…All of the isolates from vegetables in this study harbored various bla CTX-M genes, which include bla CTX-M-14 , bla CTX-M-15 , bla CTX-M-55 , bla CTX-M-65 , and bla CTX-M-27 , representing the most common ESBL types worldwide. The presence of bla CTX-M-55 , bla CTX-M-65 , and bla CTX-M-27 has also been noted in E. coli isolated from humans, animals, and retail meat in South Korea 7,8,22,24,[36][37][38] as well as in neighboring countries, including China 39,40 , Japan 41,42 , and Vietnam 43 . Despite the small number of E. coli isolates from different types of vegetables, the ESBL genotypes have been reported to be geographically distinct.…”

Section: Discussionmentioning

confidence: 90%

Extended-spectrum β-lactamase-producing Escherichia coli isolated from raw vegetables in South Korea

Song

Kim

et al. 2020

Sci Rep

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The increasing prevalence of oxyimino-cephalosporin-resistant Enterobacteriaceae has become a global concern because of their clinical impact on both human and veterinary medicine. The present study determined the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) isolates from raw vegetables. A total of 1324 samples were collected from two agricultural wholesale markets in Incheon, South Korea in 2018. The ESBL-EC strains were isolated from 0.83% (11/1324) samples, and all of them were resistant to ampicillin, piperacillin, cefazoline, cefotaxime, and nalidixic acid and yielded CTX-M-type ESBL, including CTX-M-14, CTX-M-15, CTX-M-55, CTX-M-27, and CTX-M-65. The isolates belonged to phylogenetic subgroups D (n = 5), A (n = 4), and B1 (n = 2). Multilocus sequence typing revealed nine known E. coli sequence types (STs), including ST10, ST38, ST69, ST101, ST224, ST349, ST354, ST2509, ST2847, and two new STs. Notably, ST69, ST10, ST38, and ST354 belong to the major human-associated extraintestinal pathogenic E. coli lineages. Our results demonstrate that ESBL-producing multidrug-resistant pathogens may be transmitted to humans through the vegetable intake, highlighting the importance of resistance monitoring and intervention in the One Health perspective.

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Antimicrobial Resistance and Molecular Characterization of Extended-Spectrum β-Lactamase-ProducingEscherichia coliIsolated from Ducks in South Korea

Cited by 21 publications

References 30 publications

Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Retail Meats in Korea

Extended-spectrum β-lactamase-producing Escherichia coli isolated from raw vegetables in South Korea

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