Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Retail Meats in Korea

Kim, Seokhwan; Kim, Hansol; Kim, Yong‐Hoon; Kim, Mi‐Gyeong; Kwak, Hyo‐Sun; Ryu, Sangryeol

doi:10.3390/microorganisms8040508

Cited by 11 publications

(8 citation statements)

References 76 publications

(112 reference statements)

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“…For the first time since 2011, the antimicrobial usage in Europe from 2016 to 2018 was lower in food-producing animals than it was in humans [ 19 ]. Despite this encouraging data, antimicrobial resistance needs to be monitored in animals, and in particularly, in food-producing animals [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistant E. coli in Pork and Wild Boar Meat: A Risk to Consumers

Rega

Andriani

Cavallo

et al. 2022

Foods

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Antimicrobial-resistant foodborne microorganisms may be transmitted from food producing animals to humans through the consumption of meat products. In this study, meat that was derived from farmed pigs and wild boars was analyzed and compared. Escherichia coli (E. coli) were isolated and tested phenotypically and genotypically for their resistance to quinolones, aminoglycosides and carbapenems. The co-presence of AMR-associated plasmid genes was also evaluated. A quinolone AMR phenotypic analysis showed 41.9% and 36.1% of resistant E. coli derived from pork and wild boars meat, respectively. A resistance to aminoglycosides was detected in the 6.6% of E. coli that was isolated from pork and in 1.8% of the wild boar meat isolates. No resistant profiles were detected for the carbapenems. The quinolone resistance genes were found in 58.3% of the phenotypically resistant pork E. coli and in 17.5% of the wild boar, thus showing low genotypic confirmation rates. The co-presence of the plasmid-related genes was observed only for the quinolones and aminoglycosides, but not for the carbapenems. Wild boar E. coli were the most capable to perform biofilm production when they were compared to pork E. coli. In conclusion, the contamination of pork and wild boar meat by AMR microorganisms could be a threat for consumers, especially if biofilm-producing strains colonize the surfaces and equipment that are used in the food industry.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistant E. coli in Pork and Wild Boar Meat: A Risk to Consumers

Rega

Andriani

Cavallo

et al. 2022

Foods

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“…Among mcr-1 -positive strains, eight strains exhibited a resistance phenotypes to at least two and up to 10 ( Table 3 ). In particular, two strains from domestic pork (EC2018_100) and Brazilian chicken meat (EC2017_I306) showed the extended-spectrum β-lactamase (ESBL) phenotype, which were previously reported from our group [ 31 ]. These two strains carried the bla CTX-M-55 gene and the bla CTX-M-15 gene, respectively.…”

Section: Resultsmentioning

confidence: 79%

Prevalence and Genetic Characterization of mcr-1-Positive Escherichia coli Isolated from Retail Meats in South Korea

Kim

Kang

et al. 2020

J. Microbiol. Biotechnol.

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Colistin is a cationic polypeptide antibiotic that acts against most gram-negative bacteria, including those from the family Enterobacteriaceae. Colistin is a type of polymyxin, the five known subtypes of which (A-E) lead to disruption of membrane permeability by mediating electrostatic interactions between positively charged residues of polymyxin and negatively charged lipid A components of lipopolysaccharides (LPSs) in the bacterial membrane [1]. The use of colistin, also known as polymyxin E, in clinical practice has been limited because of its nephrotoxicity and neurotoxicity [2,3]. However, owing to recent increases in multidrug resistant gram-negative bacteria and the rapid expansion of carbapenemase-producing Enterobacteriaceae, colistin has re-emerged as the last treatment option for severe bacterial infections [4,5].Several studies have reported that colistin resistance is related to chromosomal mutations in two-component systems, such as PmrAB and PhoPQ, leading to modification of LPS moieties in the outer membrane [6,7]; therefore, there was little concern regarding the spread of colistin resistance. However, since Liu and colleagues first described the plasmid-mediated transfer of colistin resistance in China in 2015 [8], the spread of the mobile colistin resistance gene has posed a serious threat to human health because of the possible emergence of bacteria resistant to all available antimicrobials [9,10].The mobile colistin resistance gene mcr-1 encodes a phosphoethanolamine transferase enzyme that is capable of modifying lipid A in the bacterial membrane and reducing the affinity for colistin [11]. Most mcr-1 genes are located on various types of plasmids, including IncI2-, IncX4-, IncHI2-, IncP-, IncY-, and IncFI1-type plasmids, and are easily transferred to other strains [12,13]. These mcr-1 genes were identified globally in various species of Enterobacteriaceae, such as Escherichia coli and Salmonella enterica isolated from humans, food animals, foods, and the environment [14]. mcr-1-positive E. coli have also been found in humans and food animals in Korea [15][16][17]. However, few studies have reported mcr-1-bearing bacteria recovered from food samples in Korea [18].Since 2003, the Korean government has monitored and surveyed the antimicrobial resistance of bacteria collected from foods, such as retail meats, within the framework of the National Program on Antimicrobial Resistance Management [19]. mcr-1-positive E. coli were recently identified from retail meat samples. In this The spread of plasmid-mediated colistin resistance has posed a serious threat to public health owing to its effects on the emergence of pandrug-resistant bacteria. In this study, we investigated the prevalence and characteristics of mcr-1-positive Escherichia coli isolated from retail meat samples in Korea. In total, 1,205 E. coli strains were isolated from 3,234 retail meat samples in Korea. All E. coli strains were subjected to antimicrobial susceptibility testing and were examined for the presence of mcr-1 gene. All ...

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“…ST88 was detected before in poultry, meat and human clinical specimens in Europe [ 36 ], and ST297 was also detected in human clinical samples [ 37 ]. ST602 was reported previously in dogs in Asia and in Europe [ 20 ], and is considered to be infrequent in humans but more frequent in animal samples [ 38 ]. To the best of our knowledge, ST1586 was not reported previously in dogs, however, it was previously reported from a horse in an equine clinic in the Netherlands [ 39 ] and from a horse in the clinic for horses at the Freie Universitat Berlin, Germany [ 40 ].…”

Section: Discussionmentioning

confidence: 98%

Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamase Producing Enterobacterales in Healthy Community Dogs in Israel

et al. 2022

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Background: antimicrobial resistance is a global problem in human and veterinary medicine. We aimed to investigate the extended spectrum β-lactamase-producing Enterobacterales (ESBL-PE) gut colonization in healthy community dogs in Israel. Methods: Rectal swabs were sampled from 145 healthy dogs, enriched, plated on selective plates, sub-cultured to obtain pure cultures, and ESBL production was confirmed. Bacterial species and antibiotic susceptibility profiles were identified. WGS was performed on all of the ESBL-PE isolates and their resistomes were identified in silico. Owners’ questionnaires were collected for risk factor analysis. Results: ESBL-PE gut colonization rate was 6.2% (n = 9/145, 95% CI 2.9–11.5). Overall, ten isolates were detected (one dog had two isolates); the main species was Escherichia coli (eight isolates), belonging to diverse phylogenetic groups—B1, A and C. Two isolates were identified as Citrobacter braakii, and C. portucalensis. A phylogenetic analysis indicated that all of the isolates were genetically unrelated and sporadic. The isolates possessed diverse ESBL genes and antibiotic-resistance gene content, suggesting independent ESBL spread. In a multivariable risk factor analysis, coprophagia was identified as a risk factor for ESBL-PE gut colonization (p = 0.048, aOR = 4.408, 95% CI 1.014–19.169). Conclusions: healthy community dogs may be colonized with ESBL-PE MDR strains, some of which were previously reported in humans, that carry wide and diverse resistomes and may serve as a possible source for AMR.

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Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Retail Meats in Korea

Cited by 11 publications

References 76 publications

Antimicrobial Resistant E. coli in Pork and Wild Boar Meat: A Risk to Consumers

Antimicrobial Resistant E. coli in Pork and Wild Boar Meat: A Risk to Consumers

Prevalence and Genetic Characterization of mcr-1-Positive Escherichia coli Isolated from Retail Meats in South Korea

Prevalence and Molecular Characterization of Extended-Spectrum β-Lactamase Producing Enterobacterales in Healthy Community Dogs in Israel

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