Extended-spectrum β-lactamase-producing Escherichia coli isolated from raw vegetables in South Korea

Song, Jang Ho; Oh, Sewon; Kim, Jung-Hee; Shin, Jin‐Wook

doi:10.1038/s41598-020-76890-w

Cited by 34 publications

(25 citation statements)

References 63 publications

(64 reference statements)

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“…E. coli isolated from fresh vegetables showed resistance to cephalosporins, such CTX and CAZ (92%) ( Ye et al, 2018 ). In our study, E. coli was most frequently resistant to cephalosporins, such as CTX and CAZ (100%), and AMP (60%), which was not only consistent with the above reports for isolates from raw vegetables but also for isolates from clinical specimens (60%–62% to cephalosporins) and healthy subjects (72% to AMP) in low- and middle-income countries ( Nji et al, 2021 ; Teklu et al, 2019 ; Ye et al, 2018 ; Song et al, 2020 ). The resistance patterns of E. coli from different sources may be associated with each other because of transmission of resistance genes between humans and environments.…”

Section: Discussionsupporting

confidence: 91%

“… Kim et al (2015) determined the antibiotic susceptibility of ESBL-producing E. coli and K. pneumoniae , isolated from sprout samples in South Korea, and showed these were resistant to CTX (100%). Another study showed that all ESBL-producing E. coli isolated from raw vegetables were completely resistant to AMP, CTX, and other antibiotics (piperacillin, cefazoline, and nalidixic acid) ( Song et al, 2020 ). E. coli isolated from fresh vegetables showed resistance to cephalosporins, such CTX and CAZ (92%) ( Ye et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from raw vegetables retailed in Southern Thailand

Romyasamit

Sornsenee

Chimplee

et al. 2021

PeerJ

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Background The increasing prevalence of broad-spectrum ampicillin-resistant and third-generation cephalosporin-resistant Enterobacteriaceae, particularly Escherichia coli and Klebsiella pneumoniae, has become a global concern, with its clinical impacts on both human and veterinary medicine. This study examined the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum β-lactamase (ESBL)-producing E. coli and K. pneumoniae isolates from 10 types of raw vegetables. Methods In total, 305 samples were collected from 9 markets in Nakhon Si Thammarat, Thailand, in 2020. Results ESBL-producing E. coli and K. pneumoniae isolates were found in 14 of the 305 samples obtained from 7 out of 10 types of vegetables (4.6% of the total). Further, 14 ESBL-producing E. coli (n = 5/14) and K. pneumoniae isolates (n = 9/14) (1.6% and 3.0%, respectively) were highly sensitive to β-lactam/carbapenem antibiotics (imipenem, 100%). ESBL-producing E. coli (n = 4) and K. pneumoniae isolates (n = 8) were also sensitive to non-β-lactam aminoglycosides (amikacin, 80.00% and 88.89%, respectively). ESBL producers were most resistant to β-lactam antibiotics, including ampicillin (85.71%) and the cephalosporins cefotaxime and ceftazidime (64.29%). The most frequently detected gene in ESBL-producing E. coli and K. pneumoniae was blaSHV. However, two ESBL-producing E. coli isolates also carried three other ESBL-encoding variants, blaTEM, blaCTX-M1, blaGES and blaTEM, blaSHV, blaCTX-M9, which may be due to their association with food chains and humans. Discussion Indeed, our results suggest that raw vegetables are an important source of ESBL-resistant E. coli and K. pneumoniae, which are potentially transmittable to humans via raw vegetable intake.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Prevalence and characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from raw vegetables retailed in Southern Thailand

Romyasamit

Sornsenee

Chimplee

et al. 2021

PeerJ

View full text Add to dashboard Cite

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“…As previously suggested, all E. coli strains can be assigned to one of the seven well-recognized phylogenetic groups, namely A, B1, B2, C, D, E, and F, as previously suggested (Clermont et al, 2013). It has been reported that E. coli phylogenetic groups A and B1 are typically commensal strains, whereas the B2 and D groups are mainly urinary tract infection strains (Song et al, 2020). In this study, based on the phylogenetic analysis, we found that antibiotic resistance was associated with virulence among E. coli strains.…”

Section: Discussionsupporting

confidence: 52%

Comparison of Antimicrobial Resistance, Virulence Genes, Phylogroups, and Biofilm Formation of Escherichia coli Isolated From Intensive Farming and Free-Range Sheep

Zhao

Adam

et al. 2021

Front. Microbiol.

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Pathogenic E. coli are among the most frequently isolated bacterial pathogens on large-scale sheep farms in China. Antibiotic use in wool sheep production is a risk factor for promoting the emergence of resistant E. coli. To reveal the differences of E. coli populations in sheep from different farming systems the antimicrobial resistance, virulence genes, biofilm formation, and phylogroups of 500 E. coli isolates obtained between September 2019 and December 2020 in northwest China from diarrheic infections of intensive farming and free-range sheep were analyzed. The antimicrobial susceptibility test for 12 classes of antimicrobial agents was determined using the broth microdilution susceptibility method, and PCR was used to detect the differences in virulence genes and phylogroups. Additionally, biofilm formation was determined using microtiter plate and slide agglutination methods. Among the 500 E. coli isolates, the majority of the isolates were multidrug resistant (75.4%) and carried at least one virulence gene (94.8%). We observed that 412 (82.4%), 360 (72.0%), and 266 (53.2%) are found to be resistant to sulfisoxazole, florfenicol, and tetracyclines, respectively. Resistance was also observed to mequindox (46.8%), ampicillin (43.6%), spectinomycin (38.6%), enrofloxacin (34.2%), ceftiofur (21.0%), gentamycin (20.4%), ceftazidime (17.8%), and polymyxin B (7.8%) but no resistance was found to meropenem. These results showed that strains from free-range subjects had fewer antibiotic resistance strains rather than sheep that were intensively farmed (P < 0.05). We observed fifteen virulence genes, of which etrA (n = 401, 80.2%) is the most common. In addition, EAEC (86.4%) is dominant among free-range sheep and EHEC (80.1%) is dominant among intensive farming. Among all virulence genes, the strongest correlation was found between etrA and papC gene (P < 0.001, OR = 455.68). Similarly, the strongest correlation was also found between eltA and sulfisoxazole (P < 0.001, OR = 877). Furthermore, the majority of the E. coli isolates belonged to phylogroup B1 (50.6%), followed by phylogroup C (20.6%), A (7.4%), E (7.4%), D (5.8%), B2 (1.6%), and F (1%). Interestingly, phylogroup B2 and D were all distributed in intensive farms. In addition, 33 (6.6%), 373 (74.6%), and 94 (18.8%) showed moderate, weak, and no connection biofilm formation ability, respectively. These data uncovered that wool sheep serve as a reservoir of pathogenic E. coli harboring multiple resistance phenotypes and virulence genes. The overlapping virulence-associated traits between IPEC and ExPEC indicated the zoonotic potential and safety threats of sheep food products. It is urgent to improve the proper use of antimicrobials in China as well as other countries.

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“…The isolates containing ST46, ST162, ST93 and ST58 were represented by one isolate each ( Figure 1 ). Recent reports documented that ST10 and ST224 E. coli are primarily carriers of CTX-M-15, CTX-M-14, CTX-M-1 and CTX-M-8 [ 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Our recent study identified ST8900 as the most prevalent ST (Sequence Typing) in CTX-M-27-positive E. coli isolates obtained during 2003 to 2009 [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

et al. 2021

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The aim of this study was to elucidate the prevalence of blaCTX-M-27-producing Escherichia coli and transmission mechanisms of blaCTX-M-27 from swine farms in China. A total of 333 E. coli isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive E. coli were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried blaCTX-M-27, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried blaCTX-M-27 genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The blaCTX-M-27 genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with blaCTX-M-27-plasmids indicated that the tra-trb region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing blaCTX-M-27 in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the blaCTX-M-27 gene in E. coli. This study indicated that the predominant vehicle for blaCTX-M-27 transmission has diversified over time and that control strategies to limit blaCTX-M-27 transmission in farm animals are necessary.

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Extended-spectrum β-lactamase-producing Escherichia coli isolated from raw vegetables in South Korea

Cited by 34 publications

References 63 publications

Prevalence and characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from raw vegetables retailed in Southern Thailand

Prevalence and characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from raw vegetables retailed in Southern Thailand

Comparison of Antimicrobial Resistance, Virulence Genes, Phylogroups, and Biofilm Formation of Escherichia coli Isolated From Intensive Farming and Free-Range Sheep

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

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