Antimicrobial Resistance and Molecular Characterization of Extended-Spectrum β-Lactamases and Other Escherichia coli Isolated from Food of Animal Origin and Human Intestinal Isolates

Abstract

Antibiotics have always appeared miraculous, saving innumerable lives. However, the unwise use of antimicrobial drugs has led to the appearance of resistant bacteria. The purpose of this study was to evaluate antimicrobial resistance in Escherichia coli (n =160) isolated from food of animal origin. The focus was on E. coli -producing extended-spectrum β-lactamases. E. coli was chosen because it is a part of the normal microbiota in mammals and can enter the food chain during slaughtering and food manipulation. Subsequently, its resistance genes can be transferred to pathogenic bacteria and human microbiota. Phenotypic and genotypic analyses of selected antimicrobial resistances were carried out together with a molecular analysis of virulence genes. E. coli isolates from food of animal origin were compared with clinical E. coli strains isolated from the human intestinal tract. Extended-spectrum β-lactamase-producing E. coli isolates were found in 9.4% of food isolates and in 1.8% of intestinal isolates. Phylogenetically, the majority of food (86.3%) and intestinal E. coli (58.1%) isolates were found to belong to the commensal phylogenetic groups A and B1. The distribution of 4 of 14 analyzed virulence factors was similar in the food and intestinal isolates. Strains isolated from food in Slovenia harbored resistance genes and virulence factors, which can constitute a problem for food safety if not handled properly.

Frequency of extended-spectrum β-lactamase (ESBL)– and AmpC β-lactamase–producing Enterobacteriaceae in a cheese production process

“…However, we determined that 83.3% of the 6 E. coli isolates in the current study were ESBL positive, similar to results reported by Özadam and Özpınar (2016), Aidara-Kane et al (2013), andAmador et al (2009). However, lower resistance rates have been reported in Germany (Odenthal et al, 2016) and Slovenia (Krizman et al, 2017). Kurekci et al (2016) also determined that 13.5% of all 87 Sürk cheese samples in Turkey contained ESBLproducing E. coli.…”

Assessment of the Presence of Resistance Genes Detected from the Environment and Selected Food Products in Benin

“…, bla CTX-M9 , and bla CTX-M15 genes was carried out using the method described in [22,23]. e amplification of fimH and iss genes was performed using the method described in [24]. Regarding PapC gene, its amplification was also carried out using the method previously described in [25].…”

Preliminary study: Health and performance assessment in broiler chicks following application of six different hatching egg disinfection protocols

“…[34] However, it is still unclear whether ESBL producing E. coli from poultry meat cause illness in humans [35], as for example a majority of isolates from healthy poultry flocks lacked virulence genes associated with human-pathogenic strains. [36] Krizman et al [37], on the other hand, found similar ESBL-producing E. coli strains in meat products and people with diarrhea. A Swedish study showed that 4.7% of the healthy population carry ESBL-producing E. coli strains with a low pathogenicity [38] as opposed to people with a blood stream infection that harbor highly pathogenic strains.…”