Frequency of extended-spectrum β-lactamase (ESBL)– and AmpC β-lactamase–producing Enterobacteriaceae in a cheese production process

Abstract

The aim of this study was to investigate the extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase activities of Enterobacteriaceae isolated from raw milk and cheese production line and to determine the probability of transmitting these bacteria to consumers. One hundred seventy-three samples from raw milk and cheese production lines were analyzed; 64 isolates were confirmed as Enterobacteriaceae. Sixteen of 64 isolates (25%) were resistant to at least one cephalosporin according to European Committee on Antimicrobial Susceptibility Testing Standards (EUCAST) and Clinical and Laboratory Standards Institute criteria. Seven of the 16 resistant isolates (43.75%) had confirmed ESBL activity. Additionally, phenotypic AmpC β-lactamase activity was observed in 31 (48.44%) of 64 Enterobacteriaceae isolates and confirmed in 27 of the 31 strains (89.1%). Overall, 3 isolates showed both ESBL activity and AmpC resistance, 28 isolates were only AmpC resistant, and 5 isolates had ESBL activity alone. Of the 173 samples, the proportions of samples that contained ESBL- and AmpC-producing isolates were 4.64 and 15.6%, respectively. Five of the ESBL-positive isolates (62.5%) and 11 of the AmpC-positive isolates (40.7%) were obtained from bulk milk tanks; therefore, the bulk tank plays a very important role in the spread of antibiotic-resistant bacteria. Periodic cleaning and maintenance of bulk tanks should be performed and recorded. Effective food safety and hygiene practices should significantly reduce cross-contamination in dairy plants.

Occurrence of the genes encoding carbapenemases, ESBLs and class 1 integron‐integrase among fermenting and non‐fermenting bacteria from retail goat meat

“…ESBL‐producing Enterobacteriaceae bacteria have been isolated using HiCrome ESBL agar in previous studies (Rameshkumar et al . 2015; Tepeli and Demirel Zorba 2017).…”

“…Thus, a total of 57 Gram-negative isolates were identified from 61 goat meat samples. ESBL-producing Enterobacteriaceae bacteria have been isolated using HiCrome ESBL agar in previous studies (Rameshkumar et al 2015;Tepeli and Demirel Zorba 2017).…”

Sığır ve Koyunlarda Plasmidik AmpC Beta Laktamaz Üreten Escherichia coli Araştırılması ve İzolatların Antibiyogram Profilleri, Filogenetik ve Virulans Karakterizasyonları

“…On the other hand, there is no report for pAmpC beta-lactamase producing E. coli in healthy sheep in Turkey. Even though some studies have shown the existence of AmpC beta-lactamase producing E. coli and AmpC genes on cattle origin food products (cheese, meat, and milk) in different parts of Turkey [35][36][37] , these studies do not reflect exactly the extent of these isolates in live animals in Turkey due to possible contamination of the animal origin food during processing. Therefore, the present study conducted have given additional information about the presence of AmpC beta-lactamase producing E. coli in cattle and sheep in Turkey.…”

Extended spectrum Beta lactamases (ESBLs): Preference for age and gender; a hospital-based study

“…They further reported that the highest proportion of ESBL producing microbials were isolated from the urine samples (62.5%) [5]. Tipelli et al [6] reported that (25%) of the isolates were resistant to at least one cephalosporin according to the recommendations of Clinical and Laboratory Standards Institute (CLSI) criteria and (43.75%) of the resistant isolates were ESBL. From Nepal limited data published in this regard showing the prevalence of ESBL as 28% [7] .…”