Preliminary study: Health and performance assessment in broiler chicks following application of six different hatching egg disinfection protocols

Tebrün, Wiebke; Motola, Gerzon; Hafez, M. H.; Bachmeier, Josef; Renfert, Kevin; Reichelt, Christian; Brüggemann-Schwarze, Sarah; Pees, Michael

doi:10.1371/journal.pone.0232825

Cited by 18 publications

(21 citation statements)

References 55 publications

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“…In a follow-up study, this observation and its potential consequences for chick development and the hatching process were investigated. Indeed, a decrease in hatchability and a reduced body weight of hatched chicks was observed after the essential oil spray treatment [55]. Therefore, the essential oils spray treatment was deemed inappropriate in practice [55].…”

Section: Plos Onementioning

confidence: 99%

“…Indeed, a decrease in hatchability and a reduced body weight of hatched chicks was observed after the essential oil spray treatment [55]. Therefore, the essential oils spray treatment was deemed inappropriate in practice [55]. On the other side, the ultrafogger method did not leave any oil residue on the eggshell surface but did not provide a satisfactory disinfection rate, when compared to the reference method.…”

Section: Plos Onementioning

confidence: 99%

“…To be seriously considered as formaldehyde alternatives, the novel approaches need to prove practicability and suitability for high numbers of hatching eggs under field conditions. Further studies were performed in which potential side effects of the different disinfection methods on foetal development were evaluated, as well as effects on hatchability and the health status of one-day chicks [55].…”

Section: Plos Onementioning

confidence: 99%

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Efficacy of six disinfection methods against extended-spectrum beta-lactamase (ESBL) producing E. coli on eggshells in vitro

Motola¹,

Hafez²,

Brüggemann-Schwarze³

2020

PLoS ONE

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The presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli on poultry products is an important issue for veterinary and human health due to the zoonotic infection risk for producers and consumers. The present study focuses on testing the efficacy of six different disinfection methods on eggshell samples, aiming to reduce ESBL producing E. coli contamination on the hatching egg. Sterile eggshell cutouts were artificially contaminated with 10 8 cfu/ml CTX-M-1 producing E. coli and used as a carrier model to analyze the efficacy of six disinfection methods. The contaminated samples were separated into two groups; 1) contaminated and disinfected, 2) contaminated and non-disinfected. Six independent disinfection protocols were performed following product specifications and protocols. Each eggshell sample was separately crushed, and the total viable bacterial count was calculated to determine the disinfection efficacy. Five out of six tested methods (formaldehyde gassing, hydrogen peroxide + alcohol spray, essential oils spray, peracetic acid foam, and low energetic electron radiation) demonstrated a reduction or completely eliminated the initial ESBL producing E. coli contamination. One method (essential oils as cold fog) only partly reached the expected efficacy threshold (reduction of >10 2 cfu/ml) and the result differed significantly when compared to the reference method i.e. formaldehyde gassing.

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Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

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Efficacy of six disinfection methods against extended-spectrum beta-lactamase (ESBL) producing E. coli on eggshells in vitro

Motola¹,

Hafez²,

Brüggemann-Schwarze³

2020

PLoS ONE

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“…Based on the results of previously conducted studies, where electron irradiation and other methods were tested on hatching eggs in an animal trial [35] without showing adverse effects on the animals, the aim of this study was to compare the health and performance parameters of hatching eggs and broilers on a larger scale after application of low energy electron irradiation versus formaldehyde fumigation on hatching eggs before incubation. The study was conducted as part of a Germany-wide research project that evaluates possibilities for the reduction of multi-resistant bacteria along the poultry production chain (EsRAM, FUZ 28177 01714).…”

Section: Resultsmentioning

confidence: 99%

“…As studies report a limited penetration depth of the electron beam [21], only a minimal radiation impact on the embryo was expected. In a previous study comparing the mutagenic effects of different disinfection protocols on chickens under laboratory conditions, the occurrence of deformed chicks was recorded as an incidental finding in some animals belonging to other treatment groups, but not in the animals treated with electron irradiation [35].…”

Section: Discussionmentioning

confidence: 99%

Use of electron irradiation versus formaldehyde fumigation as hatching egg disinfectants – efficacy and impact on hatchability and broiler performance

Pees¹,

Motola

Hafez

et al. 2020

Tierarztl Prax Ausg G Grosstiere Nutztiere

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Objective The use of low-energy electron irradiation for hatching egg disinfection was compared to formaldehyde fumigation. Material and methods To assess the efficacy, eggshells were examined for bacteria before and after disinfection. During incubation, hatching and fattening of 1400 Ross 308 broilers per group candling data, hatching rate, mortality rate and body mass development were recorded to evaluate the impact on health and performance. Additionally, data from the slaughter house and histological evaluation of organ samples were included in the health screening. Results Regarding the efficacy, irradiation and formaldehyde significantly reduced the number of bacteria compared to non-disinfected eggs. However, no significant difference was recorded between both treatments. The irradiated group performed better than the reference group concerning the number of infertile eggs, early and late dead embryos and fertile eggs. The performance during fattening was nearly identical in both groups, while mortality differed slightly between the irradiated group and the group treated with formaldehyde. Upon slaughter, there were minor variations concerning the distribution of findings between the groups, but the number of anomalies was not higher than reference values suggest. Conclusion In both groups no health problems were observed during rearing and the fattening performance met the targets of the manufacturer Aviagen for the used broiler line Ross 308. Clinical relevance Lower-energy electron irradiation proves to be an efficient and well-tolerated hatching egg disinfection procedure. Further field trials are necessary to establish low-energy electron irradiation as an alternative to formaldehyde fumigation.

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Slightly acidic electrolyzed water as an alternative disinfection technique for hatching eggs

Liu

Zheng

et al. 2022

Poultry Science

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Conventional chemical disinfectants used for egg disinfection could result in toxic residue and endanger hatchability, chick quality, and pullet growth performance. Slightly acidic electrolyzed water ( SAEW ) is known as a novel disinfectant for egg sterilization due to its high efficiency and no residue. In this study, a comprehensive assessment of slightly acidic electrolyzed water and benzalkonium bromide solution ( BBS ) used in the disinfection channel was conducted to assess the microbial count, eggshell quality, and hatchability concomitantly. The results show that the sterilization efficiency of SAEW increased with an increase in available chlorine concentration ( ACC ), spraying volume, and sterilization duration. SAEW with an ACC of 150 mg/L and 10,000 mg/L benzalkonium bromide solution had the same sterilization rates of approximately 86.2% at a spraying volume of 0.5 mL/egg and sterilization duration of 180 s. Neither had significant effect on eggshell strength or thickness. The eggshell cuticle quality in the benzalkonium bromide group was significantly higher than the control group (no disinfection) and the 150 mg/L SAEW group. The embryo weight, relative embryo weight, hatchability, and embryonic mortality in the SAEW group had no significant differences of those in the benzalkonium bromide group. SAEW should be more popular because of its simple preparation, low cost, and no residue. Our results indicate SAEW is an alternative disinfectant for the sterilization of hatching eggs instead of conventional chemical disinfectants, such as benzalkonium bromide, and give a recommendation is using SAEW as a disinfectant with 150 mg/L ACC, 0.5 mL/egg spray volume, and disinfection for 180 s in the novel disinfection channel.

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Preliminary study: Health and performance assessment in broiler chicks following application of six different hatching egg disinfection protocols

Abstract: Preliminary study: Health and performance assessment in broiler chicks following application of six different hatching egg disinfection protocols.

Cited by 18 publications

References 55 publications

Efficacy of six disinfection methods against extended-spectrum beta-lactamase (ESBL) producing E. coli on eggshells in vitro

Efficacy of six disinfection methods against extended-spectrum beta-lactamase (ESBL) producing E. coli on eggshells in vitro

Use of electron irradiation versus formaldehyde fumigation as hatching egg disinfectants – efficacy and impact on hatchability and broiler performance

Slightly acidic electrolyzed water as an alternative disinfection technique for hatching eggs

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