Antimicrobial potential of medicaments used in healing process in dogs' teeth with apical periodontitis

Abstract

The objective of this study was to evaluate the influence of irrigants on the antimicrobial potential of calcium hydroxide paste in dogs' teeth with apical periodontitis. Forty-eight premolar teeth of adult mongrel dogs had their root canals opened to the oral environment for 6 months. The root canals were prepared and treated with different irrigating solutions and intracanal medicaments, according to the following groups: 1) 2.5% sodium hypochlorite (NaOCl) + calcium hydroxide paste (CHP); 2) 2% chlorhexidine (CHX) + CHP; 3) vinegar + CHP; 4) vinegar + vinegar. In group 4, both the irrigating solution and intracanal medicament were vinegar, which was renewed every 7 days. Each sample was collected by using two paper points maintained in position for 1 min, and individually transported and immersed in 7 mL of Letheen broth (LB), followed by incubation at 37 o C for 48 h. Microbial growth was analyzed by two methods: turbidity of the culture medium and subculture on a specific nutrient broth (brain heart infusion). After 21 days, all experimental groups had microbial growth, however, in different percentages: group 1 -30%; group 2 -30%; group 3 -40%; group 4 -60%. All materials tested had antimicrobial potential; however, the influence of calcium hydroxide paste on the control of microorganisms must be remembered.

Efficacy of NiTi rotary instruments in removing calcium hydroxide dressing residues from root canal walls

“…Ibiporã, Brazil) and propylene glycol (Synth, Diadema, Brazil) paste using a lentulo spiral (Dentsply Maillefer, Ballaigues, Switzerland), at a powder-to-liquid ratio of 1 g : 1.5 mL. 23 Radiographs were taken of each root to confirm their complete filling with calcium hydroxide paste. The teeth used for negative control (n = 2) received no calcium hydroxide intracanal dressing, and those used for positive control (n = 2) received the intracanal dressing, but no following removal procedure was carried out.…”

Estudo longitudinal do sucesso clínico-radiográfico de dentes tratados com medicação intracanal de hidróxido de cálcio

“…A medicação intracanal empregada em forma de pasta com veículo aquoso, neste estudo, pode ser justificada por estudos 7,8,[25][26][27] que demostram que as propriedades do hidróxido de cálcio derivam de sua dissociação em íons cálcio e íons hidroxila. A ação desses íons sobre os tecidos e microorganismos proporcionou propriedades osteogênicas, além das ações neutralizadora e antibacteriana.…”

“…A ação desses íons sobre os tecidos e microorganismos proporcionou propriedades osteogênicas, além das ações neutralizadora e antibacteriana. Considerando-se que a liberação dos íons cálcio e hidroxila na forma aquosa é mais rápida, acentuada, contínua e provoca a alcalinização necessária ao combate aos micro-organismos a partir de duas semanas de seu emprego 27 , valida-se esta escolha pela medicação intracanal de hidróxido de cálcio.…”

“…biológico dos cimentos à base de hidróxido de cálcio, que colaboram para o reparo das lesões periapicais, justificando assim tal preferência pelo Sealapex 2,25,26 . A obturação do canal radicular com o cimento Sealapex manteria o pH em níveis alcalinos, contribuindo, desta forma, para que a eliminação dos micro-organismos se completasse, acelerando assim o reparo da lesão periapical 2,7,8,26,27 .…”

Comparison of the Impact of Long-Term Applications of MTAD and EDTA Irrigation Agents on Dentin Structure: A Scanning Electron Microscope Study

“…Several chelating agents have been recommended for the removal of the smear layer, including BioPure MTAD (Dentsply/Tulsa, Tulsa, OK, USA), ethylenediaminetetraacetic acid, citric acid, EDTAC, REDTA, Glyde files, apple cider vinegar, and RC-Prep (8)(9)(10)(11)(12)(13)(14). EDTA effectively removes the smear layer and is one of the most preferred chelating agents in endodontics.…”