Antimicrobial potential of AH Plus supplemented with bismuth lipophilic nanoparticles on E. faecalis isolated from clinical isolates

Abstract

The objective of this study was to determine the antimicrobial potential of AH plus supplemented with bismuth lipophilic nanoparticles (BisBAL NPs) on the growth of Enterococcus faecalis isolated from patients with endodontic infections. BisBAL NPs, synthesized with the colloidal method, were characterized, in its pure form or AH Plus-absorbed, by energy-dispersive X-ray spectroscopy and scanning electron microscopy (EDS-SEM). Antimicrobial activity was evaluated with disc diffusion assays, and antibiofilm activity with fluorescence microscopy. BisBAL NP-supplemented AH Plus had a 4.9 times higher antimicrobial activity than AH Plus alone ( p = 0.0001). In contrast to AH Plus alone, AH Plus supplemented with BisBAL NP inhibited E. faecalis biofilm formation. The sealing properties of AH plus were not modified by the incorporation of BisBAL NPs, which was demonstrated by a 12-day split-chamber leakage assay with daily inoculation, which was used to evaluate the possible filtration of E. faecalis. Finally, BisBAL NP-supplemented AH plus-BisBAL NPs was not cytotoxic for cultured human gingival fibroblasts. Their viability was 83.7% to 89.9% after a 24-h exposure to AH Plus containing 50 and 10 µM BisBAL NP, respectively. In conclusion, BisBAL NP-supplemented AH Plus constitutes an innovative nanomaterial to prevent re-infection in endodontic patients without cytotoxic effects.

Effect of Vitapex Combined with AH-Plus Paste on Inflammation in Middle-Aged and Elderly Patients with Periodontal-Endodontic Disease

“…The reasons are as follows. Root canal filling is an important method for the clinical treatment of periodontal-endodontic disease, and the choice of filling material has a great influence on the treatment effect [15]. As a new type of root canal filling material, Vitapex paste is mainly composed of calcium hydroxide, polysiloxane oil, and iodoform.…”

RETRACTED ARTICLE: The role of bismuth nanoparticles in the inhibition of bacterial infection

“…Drugs containing Bi-based chemicals have found widespread application in the treatment of gastrointestinal diseases such as gastric ulcers, dyspepsia, and H. pylori infections. Nowadays, their medical applications have been expanded to include imaging, medication delivery, biosensing, and the treatment of viral infections, MRD microbial infections, cancer, and more (Griffith et al 2021 ; Betancourt et al, 2022 ). Antimicrobial activities of BiNPs have been established in several lab experiments, and they have been successfully utilized to treat H. pylori ulcers in humans.…”