Antimicrobial photodynamic therapy with RLP068 kills methicillin‐resistant <i>Staphylococcus aureus</i> and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion

Vecchio, Daniela; Huang, Liyi; Fantetti, Lia; Roncucci, Gabrio; Hamblin, Michael R.

doi:10.1002/jbio.201200121

Cited by 88 publications

(79 citation statements)

References 33 publications

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“…All the mice with wounds were divided into following groups: (1) uninfected, (2) uninfected, PDT given once on day 2 p.w., (3) infected untreated control, (4) infected, PDT given at 24-h intervals during days 1 to 3 p.w. (multiple PDT), (5) infected AgNO 3 and (6) infected AG.…”

Section: Photodynamic Therapy and Other Treatment Of Woundsmentioning

confidence: 99%

“…Antimicrobial photodynamic therapy (APDT), which makes use of photoexcitation of a photosensitive drug to inactivate bacteria is receiving a lot of attention for the management of antibiotic-resistant bacteria infection in wounds [1][2][3]. PDT, in addition to the antibacterial effect, has also been shown to have influence on several other factors like bacterial toxins [4], proinflammatory cytokines [5], matrix metalloproteases, collagen synthesis, and remodeling [6] in wounds.…”

Section: Introductionmentioning

confidence: 99%

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Topical antimicrobial photodynamic therapy improves angiogenesis in wounds of diabetic mice

et al. 2015

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We report the results of our investigations on the effect of antimicrobial photodynamic therapy (APDT) on angiogenesis in wounds of diabetic mice. For this, measurements were made on levels of nitric oxide (NO), vascular endothelial growth factor-A (VEGF-A), and markers of proinflammatory stress (phosphorylated nuclear factor kappa B and p(38) mitogen-activated protein kinase) on day 3 post-wounding. For uninfected and infected wounds, the levels of NO, VEGF-A were lower and the levels of phospho-NF-kB-p65, phospho-p(38)MAPK were higher in diabetic mice compared with that in nondiabetic mice. For infected wounds, multiple APDT (fluence ~60 J/cm(2)) led to increase in NO, VEGF-A levels and a decrease in the phospho-NF-kB-p65, phospho-p(38)MAPK. Further, compared with aminoguanidine, and silver nitrate, multiple APDT was observed to result in a much improved proangiogenic response.

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Section: Photodynamic Therapy and Other Treatment Of Woundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Topical antimicrobial photodynamic therapy improves angiogenesis in wounds of diabetic mice

et al. 2015

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“…Furthermore a multiple positive charged tetracationic Zn(II)phthalocyanine was able to kill bacteria but not TBO which was used as a reference photosensitizer [50].…”

Section: Porphyrins and Phthalocyaninesmentioning

confidence: 99%

Strategies to optimize photosensitizers for photodynamic inactivation of bacteria

Maisch

2015

Journal of Photochemistry and Photobiology B: Biology

156

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“…42 PDT may be a viable option in the treatment of biofilm-infected wounds through microbial cell death, reduction in bacterial proteases, and the promotion of wound closure. 43,44 Bacterial proteases as therapeutic targets…”

Section: Bacterial Proteases As Diagnostic Markers In Chronic Woundsmentioning

confidence: 99%

Extracellular Bacterial Proteases in Chronic Wounds: A Potential Therapeutic Target?

Suleman

2016

Advances in Wound Care

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Significance: Bacterial biofilms are considered to be responsible for over 80% of persistent infections, including chronic lung infections, osteomyelitis, periodontitis, endocarditis, and chronic wounds. Over 60% of chronic wounds are colonized with bacteria that reside within a biofilm. The exaggerated proteolytic environment of chronic wounds, more specifically elevated matrix metalloproteinases, is thought to be one of the possible reasons as to why chronic wounds fail to heal. However, the role of bacterial proteases within chronic wounds is not fully understood. Recent Advances: Recent research has shown that bacterial proteases can enable colonization and facilitate bacterial immune evasion. The inhibition of bacterial proteases such as Pseudomonas aeruginosa elastase B (LasB) has resulted in the disruption of the bacterial biofilm in vitro. P. aeruginosa is thought to be a key pathogen in chronic wound infection, and therefore, the disruption of these biofilms, potentially through the targeting of P. aeruginosa bacterial proteases, is an attractive therapeutic endeavor. Critical Issues: Disrupting biofilm formation through the inhibition of bacterial proteases may lead to the dissemination of bacteria from the biofilm, allowing planktonic cells to colonize new sites within the wound. Future Directions: Despite a plethora of evidence supporting the role of bacterial proteases as virulence factors in infection, there remains a distinct lack of research into the effect of bacterial proteases in chronic wounds. To assess the viability of targeting bacterial proteases, future research should aim to understand the role of these proteases in a variety of chronic wound subtypes.

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Antimicrobial photodynamic therapy with RLP068 kills methicillin‐resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion

Cited by 88 publications

References 33 publications

Topical antimicrobial photodynamic therapy improves angiogenesis in wounds of diabetic mice

Topical antimicrobial photodynamic therapy improves angiogenesis in wounds of diabetic mice

Strategies to optimize photosensitizers for photodynamic inactivation of bacteria

Extracellular Bacterial Proteases in Chronic Wounds: A Potential Therapeutic Target?

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