Antimicrobial peptides-loaded smart chitosan hydrogel: Release behavior and antibacterial potential against antibiotic resistant clinical isolates

Rezaei, Nourollah; Hamidabadi, Hatef Ghasemi; Khosravimelal, Sadjad; Zahiri, Maria; Ahovan, Zahra Aliakbar; Bojnordi, Maryam Nazm; Eftekhari, Behnaz Sadat; Hashemi, Ali; Ganji, Fatemeh; Darabi, Shahram; Gholipourmalekabadi, Mazaher

doi:10.1016/j.ijbiomac.2020.07.011

Cited by 63 publications

(46 citation statements)

References 51 publications

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“…Due to their antimicrobial and biological potentials, these peptides have also been considered to wound dressing purposes. [8,34,35]. Accordingly, many investigations to be designed for controlled-release of such peptides with considerable antibacterial activity especially for emerging resistant strains without inducing cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an increasing number of antimicrobial-loaded scaffolds have been introduced in several formats like hydrogels [8] and electrospun membranes [7] as in novel methods. Among them, electrospun bers have been used in biomedical applications in a wide range as they can act like a natural extracellular matrix (ECM) for cell motility and propagation.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and Characterization of an Antibacterial Chitosan/Silk Fibroin Electrospun Nanofiber Loaded with a Cationic Peptide for Wound-dressing Application

Khosravimelal

Chizari

Farhadihosseinabadi

et al. 2021

Preprint

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Wound infections are still problematic in many cases and demand new alternatives for current treatment strategies. In recent years, biomaterials-based wound dressings have received much attention due to their potentials and many studies have been performed based on them. Accordingly, in this study, we fabricated and optimized an antibacterial chitosan/silk fibroin (CS/SF) electrospun nanofiber bilayer containing different concentrations of a cationic antimicrobial peptide (AMP) for wound dressing applications. The fabricated CS/SF nanofiber was fully characterized and compared to the electrospun silk fibroin and electrospun chitosan alone in vitro. Then, the release rate of different concentrations of peptide (16, 32, and 64 µg/ml) from peptide-loaded CS/SF nanofiber was investigated. Finally, based on cytotoxic activity, the antibacterial activity of scaffolds containing 16 and 32 µg/ml of peptide was evaluated against standard and multi-drug resistant strains of Staphylococcus aureus and Escherichia coli isolated from burn patients. The peptide-loaded CS/SF nanofiber displayed appropriate mechanical properties, high water uptake, suitable biodegradation rate, a controlled release without cytotoxicity on Hu02 human foreskin fibroblast cells at the 16 and 32 µg/ml concentrations of peptide. The optimized CS/SF containing 32 µg/ml peptide showed strong antibacterial activity against all experimental strains from standard to resistant. The results showed that the fabricated antimicrobial nanofiber has the potential to be applied as a wound dressing for infected wound healing, although further studies are needed in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of an Antibacterial Chitosan/Silk Fibroin Electrospun Nanofiber Loaded with a Cationic Peptide for Wound-dressing Application

Khosravimelal

Chizari

Farhadihosseinabadi

et al. 2021

Preprint

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“…Rezaei et al have prepared thermo-responsive chitosan (TCTS) hydrogels and loaded them with piscidin-1 AMP at 16 μg/mL to obtain efficient antibacterial wound dressing against resistant strains of A. baumannii . The use of TCTS hydrogels as a carrier for piscidin-1 showed to be advantageous with high antibacterial activity and an absence of toxicity to mammal cells [ 193 ].…”

Section: Nanotechnological Platforms and Scaffolds For Peptide Delmentioning

confidence: 99%

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

2020

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Bacterial infections occur when wound healing fails to reach the final stage of healing, which is usually hindered by the presence of different pathogens. Different topical antimicrobial agents are used to inhibit bacterial growth due to antibiotic failure in reaching the infected site, which is accompanied very often by increased drug resistance and other side effects. In this review, we focus on antimicrobial peptides (AMPs), especially those with a high potential of efficacy against multidrug-resistant and biofilm-forming bacteria and fungi present in wound infections. Currently, different AMPs undergo preclinical and clinical phase to combat infection-related diseases. AMP dendrimers (AMPDs) have been mentioned as potent microbial agents. Various AMP delivery strategies that are used to combat infection and modulate the healing rate—such as polymers, scaffolds, films and wound dressings, and organic and inorganic nanoparticles—have been discussed as well. New technologies such as Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated protein (CRISPR-Cas) are taken into consideration as potential future tools for AMP delivery in skin therapy.

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“… 59 Amariei et al developed a wound dressings with antibacterial peptides electrospun submicron fibers from poly(acrylic acid)‐poly(vinyl alcohol) blends for performed using SA , then tested have a good resistance against SA 60 . Rezaei et al designed and fabricated AMP‐loaded CS hydrogel against a common drug resistant bacterial isolate, which recommended for healing management usage as an excellent antibacterial wound dressing against resistant Acinetobacter baumannii AB 61 …”

Section: Polymersmentioning

confidence: 99%

“…60 Acinetobacter baumannii AB. 61 The antibacterial properties of EPL-based polymers were also evaluated by some researchers. 62,63 MRSA bacteria, besides it is especially recommended for burn treatment that showed excellent biocompatibility.…”

Section: Polymersmentioning

confidence: 99%

Advancements and future directions in the antibacterial wound dressings – A review

et al. 2020

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Wound repair is a complex process that has not been entirely understood. It can conclude in several irregularities. Hence, designing an appropriate wound dressing that can accelerate the healing period is critical. Infections, a major obstacle to wound repair, cause an elevated inflammatory responses and result in ultimate outcome of incomplete and prolonged wound repair. To overcome these shortcomings, there is a growing requirement for antibacterial wound dressings. Dressings with antibacterial activities and multifunctional behaviors are highly anticipated to avoid the wound infection for successful healing. The aim of this review is not only to concentrate on the importance of antibacterial dressings for wound healing applications but also to discuss recent studies and some future perspectives about antibacterial wound dressings.

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Antimicrobial peptides-loaded smart chitosan hydrogel: Release behavior and antibacterial potential against antibiotic resistant clinical isolates

Cited by 63 publications

References 51 publications

Fabrication and Characterization of an Antibacterial Chitosan/Silk Fibroin Electrospun Nanofiber Loaded with a Cationic Peptide for Wound-dressing Application

Fabrication and Characterization of an Antibacterial Chitosan/Silk Fibroin Electrospun Nanofiber Loaded with a Cationic Peptide for Wound-dressing Application

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

Advancements and future directions in the antibacterial wound dressings – A review

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