Antimicrobial peptide incorporated poly(2‐hydroxyethyl methacrylate) hydrogels for the prevention of Staphylococcus epidermidis‐associated biomaterial infections

Abstract

The effectiveness of the antimicrobial peptide maximin‐4, the ultrashort peptide H‐Orn‐Orn‐Trp‐Trp‐NH2, and the lipopeptide C12‐Orn‐Orn‐Trp‐Trp‐NH2 in preventing adherence of pathogens to a candidate biomaterial were tested utilizing both matrix‐ and immersion‐loaded poly(2‐hydroxyethyl methacrylate) (poly(HEMA)) hydrogels. Antiadherent properties correlated to both the concentration released and the relative antimicrobial concentrations of each compound against Staphylococcus epidermidis ATCC 35984, at each time point. Immersion‐loaded samples containing C12‐Orn‐Orn‐Trp‐Trp‐NH2 exhibited the lowest adherence profile for all peptides studied over 1, 4, and 24 h. The results outlined in this article show that antimicrobial peptides have the potential to serve as an important weapon against biomaterial associated infections. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

Controlled Release of Plectasin NZ2114 from a Hybrid Silicone-Hydrogel Material for Inhibition of Staphylococcus aureus Biofilm

“…The use of AMPs in combination with medical devices to prevent device-associated infections is, however, an enticing alternative application that to a lesser extent is restricted by the above problems (3,4,5).…”

Antibacterial activity on electrospun poly(lactide-co-glycolide) based membranes via Magainin II grafting

“…Synthetic approaches like metal derivatives [21] and antibiotics [22] have been used to produce antibacterial/bactericidal layer on the surface of material. Apart from these synthetic approaches, bio-inspired natural substances such as antimicrobial peptides [12][13][14][15][16][17], bacteriolytic enzymes [29] and essential oils [19] have recently gained importance to overcome biofilm-associated infections. Among these natural strategies antimicrobial peptides (AMPs) have been increasing as an alternative possessing a broad spectrum of antimicrobial activity [1,2].…”

“…The physicochemical characteristics of AMPs enable them to associate with negatively charged microbial membranes resulting in a lethal disruption in cell membrane [2]. In recent works, covalent immobilization of AMPs on different solid supports has been discussed for long-term stability and accessibility towards bacterial cells [3,[12][13][14][15][16][17].…”

Non-biodegradable polymer particles for drug delivery: A new technology for “bio-active” restorative materials

“…Because Triton X-100 hinders hydrophobic interaction 56,57) , CPC adsorbed to polymers through hydrophobic interaction is desorbed by this surfactant. An NaCl solution is effective to assess the involvement of electrostatic interaction in the binding of CPC to polyHEMA/TMPT since CPC is cationic and shows strong interaction with negatively charged substances 27) .…”

“…The equilibrium mass swelling ratio (q) in solvent×(I70: 70 vol% isopropanol, H2O: distilled water) was calculated as: qx=Ws/Wd, and the H-index was determined as: H=qI70/qH2O. groups 26,27) . The zeta potential of each polymer particle was measured, and all five polymers presented a negative surface charge but their charges were not significantly different (Table 1).…”