Combertum indicum has two varieties, namely varr.M and Varr.B., so to maintain the truth of the sample it is necessary to discriminate. The way to distinguish these two varieties can use the UV-vis spectrophotometric approach. The method used in chemometrics uses five sampling districts, namely Banjarmasin, Banjarbaru, Banjar, Tanah Laut, Tanah Spice, the equipment used is spectrophotometry UV-vis. Data analysis uses descriptive analysis. The results of the identification of flower performance index obtained are 88.9; Leave identification 92.2; stem identification 86.8. In conclusion, the combination chemometric UV-vis spectrophotometry succeeded in differentiating combertum indicum varr.M and Varr.B.