ANTIMICROBIAL EFFECT OF ARROWROOT (Maranta arundinacea L.) METHANOLIC EXTRACT AGAINST Staphylococcus aureus BACTERIAL GROWTH

Abstract

Introduction: Staphylococcus aureus is a spherical gram-positive bacterium that forms clusters and is frequently resistant to antibiotics. Methicillin-Resistant Staphylococcus aureus (MRSA) is a strain of S. aureus that is resistant to beta-lactam and cephalosporin groups of antibiotics, it is one of the biggest problems in resistant bacteria. Plants that have flavonoids in their extract can inhibit the growth of bacteria by impairing their cytoplasmic membrane. Arrowroot is an example of plants that have flavonoids in their extracts. Objectives: This research aims to determine the antimicrobial effect of arrowroot (Maranta arundinacea L.) methanolic extract against the growth of Staphylococcus aureus in vitro. Methods: This is true experimental research with a post-test control group design with two control groups and eight experimental groups. The sample of this research utilizes the MRSA strain of S. aureus received from the Microbiology Laboratory of Diponegoro University Faculty of Medicine that was eligible with the inclusion criteria. The research was conducted by using arrowroot extract concentrations 6.5%, 12.5%, 25%, 50%, and 100% to determine the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and inhibitory zone diameter. Results: MIC and MBC of arrowroot methanolic extract against MRSA is 100%. The arrowroot methanolic extract to have yielded an inhibitory zone diameter is 100% with a mean inhibitory zone diameter of 15.5mm. Conclusion: Arrowroot methanolic extract can inhibit the bacterial growth of MRSAKeywords: Staphylococcus aureus, growth, M. arundinacea L. methanolic extract

O meio ambiente em foco: desafios e soluções para a sustentabilidade

“…Quando comparadas todas as diluições, pode-se observar maior eficiência de redução do rizoma. O potencial microbicida da araruta é decorrente, entre outros, das altas concentrações de flavonoides no rizoma e folha, que inibem o crescimento bacteriano, prejudicando a membrana plasmática desses microrganismos (SYAHPUTRA et al, 2020).…”

Ararot (Maranta Arundinacea L.) Ri̇zomlarinin Fi̇toki̇myasal, Tibbi̇ Ve Besi̇nsel Özelli̇kleri̇ Ve Çeşi̇tli̇ Kullanimlari

“…Rizom ve rizomdan elde edilen ürünlerin antioksidan, immünostimulan, anti-ülserojenik [9,16,17,24], antidiyareik [5,9,17,35], anti-inflamatuvar, antimikrobiyal [13][14][15][16][17] (antibakteriyal [9,16,52], antifungal [26], vibriosidal [9,24]), antidispeptik, antihipertansif, hipokolesterolemik, hipoglisemik [6,13], antikanser [32] ve hepatoprotektif [24] aktiviteleri rapor edilmiştir. Literatürde rizomların metanol ekstresi in vitro antioksidan ve antibakteriyal; etanol ekstresi in vitro antioksidan ve anti-inflamatuvar ve in vivo antioksidan ve prebiyotik; rizom infüzyonu hipoglisemik; rizomdan elde edilen nişasta antidispeptik ve in vivo anti-ülseratif; rizomlar hipoglisemik ve rizom tozu antidiyareik, immünomodülatör ve in vivo prebiyotik etkili bulunmuştur.…”

“…Genç rizomların antimikrobiyal aktiviteye sahip olduğu bilinmektedir [12]. Rizomların ve rizomlardan elde edilen ürünlerin antimikrobiyal etkinliği birçok mikrobiyal suşa (Staphylococcus aureus [16,52], Staphylococcus epidermidis [16], Escherichia coli [16,53], Clostridium perfringens [53], Streptococcus mutans, Salmonella sp., Bacillus cereus [16], Lactobacillus sp. [16,53,54] (L. acidophilus [13,55], L. plantarum [34], L. casei [16] (rhamnosus ve shirota suşları)), Bifidobacterium bifidum, Bifidobacterium longum [16]) karşı test edilmiş ve etkili bulunmuştur.…”

“…Rizomların Tinea Pedis'i tedavi etme etkisi antifungal aktivitesine bağlanmıştır [26]. Yapılan çeşitli araştırmalarda ararot rizom ekstresinden hazırlanan gümüş nanopartiküllerin in vitro olarak S. aureus ve E. coli'nin [16]; ararottan hazırlanan selenyum nanoparteküllerin S. mutans'ın [16] ve rizomların metanol ekstresinin metisiline dirençli S. aureus'un büyümesini inhibe ettiği bildirilmiştir [52]. Ararot unu içeren diyet ile beslenen farelerde yapılan in vivo çalışmada C. perfringens ve E. coli popülasyonlarının kontrol grubuna göre fark önemsiz olmakla birlikte daha düşük olduğu bulunmuştur [53].…”

Arrowroot (Maranta arundinacea L.) as a new potential functional food: A scoping review

“…Selenium nanoparticles from MA inhibited Streptococcus mutans and Lactobacillus (Balamithra et al, 2020). Additionally, the methanolic extract of MA inhibited methicillin-resistant Staphylococcus aureus (Syahputra et al, 2020). The growth of E. coli, Salmonella, and Bacillus cereus was reduced by 3.2, 1.5 -3.9, and 1.4 -3.5 log CFU/mL, respectively, when compared to Lactobacillus casei rhamnosus strain in media containing MA extract (Dwiari, 2008).…”