Antimicrobial, Antioxidant and some Biochemical Properties of Vaccinium vitis-idaea L.

Abstract

Over the past years there has been a growing interest to vegetable raw materials evaluated from the viewpoint of their potential antimicrobial activity. Vaccinium genus plants are known to contain a whole spectrum of biologically active substances with anti-inflammatory, antidiabetic, gene-protective, antioxidant and antimicrobial properties. The purpose of the work was to study the antimicrobial, antioxidant and some biochemical properties of alcoholic extracts of fruit and leaves of Vaccinium vitis-idaea L., gathered in the Ukrainian Carpathians. Methods. The plants for the study were gathered around the village of Pylypets, Mizhhiria rayon, Zakarpatska oblast (Transcarpathia). From the fruit and leaves, ethyl and methyl extracts were produced. The subjects for study were their antioxidant activity (by DPPH method), total tannin and flavonoids (by spectrophotometric method), and antimicrobial activity (by diffusion-into-agar method). For the purpose of study, type and clinical isolates were used from the oral cavity of human patients suffering from inflammatory diseases of periodontium, characterized by wide resistance spectrum to antibiotics. Results. The reviewed extracts were observed to have a higher antimicrobial activity against Gram-positive bacteria. The highest antimicrobial activity was shown by the extract of Vaccinium vitis-idaea L. fruit. Its antimicrobial activity was registered upon both test and clinical isolates of S.aureus, including methicillin-resistant ones. The ethyl fruit extract and methanol leaf extract were established to show high antimicrobial effect upon S.pyogenes. The ethanol fruit and leaf extracts and methanol fruit extract were observed to show weak antimycotic activity. The extracts were characterized by high antioxidant activity, with the highest effect peculiar for ethyl leaf and fruit extracts, and somewhat lower one for methyl extracts. The ethanol fruit extract was characterized by the highest tannin level out of all extracts under review. Conclusions. The antimicrobial and high antioxidant activity of the ethanol extract of Vaccinium vitis-idaea L. fruit and leaves, and absence of the antimicrobial effect against the probiotic strain of Lactobacillus plantarum, characterized by the antagonistic activity against opportunistic microorganisms, cause the advisability of their complex application as the basis for phytobiotics.

Anty-microbial, and anty-biofilm-forming properties of Origanum vulgare L. essential oils on Staphylococcus aureus and its antioxidant action

“…Their antimicrobial activity is due to the ability to disrupt the structure of the cytoplasmic membrane, which leads to the disruption of the transport of substances and metabolic processes [30]. Medicinal plants and essential oils attract attention as sources for the production of drugs with antimicrobial, anti-inflammatory and immunomodulatory effects [15,21].…”

Effect of Fresh and Dried Blueberry (Vaccinium myrtillus) Fruit and Leaf Extracts on Probiotics and Pathogens

“…Kryvtsova ve ark. [39] ise Vac. vitis-idaea türünün meyve ve yapraklarına ait etanol ve metanol ekstraktlarının S. aureus ATCC 25923 ve E. coli ATCC 25922 üzerindeki antimikrobiyal etkisinin karşılaştırıldığı bir çalışmada, meyve etanol ekstraktları ile yaprak metanol ekstraktlarının yüksek antimikrobiyal etki (S. aureus) gösterdiği, fırsatçı mikroorganizmalara karşı antogonistik etki gösteren probiyotik türler (L. plantarum) üzerinde aynı etkiye sahip olmadığını tespit etmişlerdir.…”

“…myrtillus L.'e ait yaprak ve meyve metanol ekstratlarının S. aureus ve E. coli ATCC 9863 üzerindeki antimikrobiyal etkisinin incelendiği bir diğer çalışmada ise S. aureus'un en hassas mikroorganizma olduğu belirlenmiştir [6]. Üzümsü meyvelerden elde edilen fenolik ekstraktların; molekül yapılarının küçük olmasından dolayı hücre zarından kolaylıkla geçip hücre içinde çözünerek antimikrobiyal etki gösterdikleri, bu etkinin mikroorganizmaların Gr (+) veya Gr (-) olmalarına bağlı olarak farklı sonuçlar gösterdiği, ekstraktın bitkinin hangi kısmından elde edildiğine, bitkinin yetiştirilme koşulları, toplanma zamanı ve lokasyonu, kurutma koşulları, ekstraksiyonda kullanılan çözücünün çeşidi, polaritesi, konsantrasyon, ekstraksiyon koşullarıyla bitkinin fenolik madde miktarına bağlı olduğu ifade edilmektedir [3][4][30][31][32][33][34][35][36][37][38][39][40][41].…”