Antimicrobial and Antiviral Activity of Lucilia sericata, Chrysomya albiceps (Diptera: Calliphoridae) And Musca domestica (Diptera: Muscidae) Whole Body Extract

Abstract

The present study dealt with evaluating the effect of whole body extracted by ethyle acetate of L. sericata, C. albiceps and M. domestica as exhibited antibacterial activity against some species of bacteria. They were shown to inhibit the growth of Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, Gram-negative bacteria, Escherichia coli and Klebsiella pneumoniae and Fungi, Aspergillus fumigatus thus exhibited limited inhibitory effect towards Gram-positive bacteria Bacillus subtilis and Gram-negative Pseudomonas aeruginosa and Fungi Syncephalastrum racemosum and Candida albicans, this effect was slowed down when challenged with secretion on a solid media but no zone of complete inhibition was detected. Growth inhibiting activity was determined in liquid growth media using the Grampositive, Gram-negative bacterial and fungal strains as indicator organisms. The antiviral activity of whole body extracted by ethyle acetate of L. sericata, C. albiceps and M. domestica nES were tested against Vero cell line was developed for isolation and propagation of many enteroviruses and hepatitis A and average values were considered.

Microbial Stress Resistance of Eristalis tenax Rat-Tailed Maggots

“…The antibacterial activity results showed that, tested extracts evoked a high activity against both Gram-positive and Gram-negative comparing to many insects crude extract. (Leem et al, (1999), Thomas et al, (1999), Yamauchi,(2001 and Amer et al, (2019). Positive bacterial strains were more sensitive to the tested maggots' extracts than Gram-negative bacterial strains.…”

Reproductive Potential and Embryonic Lethality of The Blowfly, Chrysomya albiceps (Wiedemann 1819)Influenced by Gamma Radiation

“…Maggots after hatching were transferred into a plastic box (25×15×10 cm) containing fresh liver and covered by gauze, larvae were fed on the liverwhen maggots were ready to start pupation, they left the moist diet into a box containing sawdust that was spread on top. Then the pupae were transferred into wooden cages (Amer et al,2019).…”

Antiviral activity of the hemolymph ofPodalia sp and M. albicolis(Lepidoptera: Megalopigydae)

“…Invertebrates A likely source of antivirals are the invertebrates. They do not have a developed immune system as in mammals, but the hemolymph of these artropods contains substances with potent antibacterial action (Amer et al, 2019., Oliveira et al, 2019., Díaz-Roa et al, 2019., Diniz et al, 2018, Díaz-Roa et al, 2018, Abreu, et al, 2017, Candido-Ferreira et al, 2017, Chaparro and Junior, 2016, Sayegh et al, 2016., Pavillard and Wright, 1957), virus (Olicard et al, 2005a., Olicard et al, 2005b., Dang et al, 2006, 2011, Mondotte et al, 2018., Cociancich et al, 1994., Ferrandon et al, 1998, Vistnes et al, 1981) fungal (Lamberty et al, 1999, Lauth et al, 1998., Fehlbaum, et al 1994 or parasitoid, infestations (Whitman et al 2019., Lacerda et al, 2016., Marr et al, 2012., Bell, 2011, Rangel et al, 2011, Gao et al, 2010, Fieck et al, 2010., Pulido et al, 2008. In invertebrates, the protector responses are based in the peptide with antimicrobial action (Imuler and Hoffmann, 2000; Krutzik et al, 2001; Underkill and Orinsky, 2002), hemolymph coagulation (Iwanaga et al, 1978), melanin formation (Sugumaran, 2002), and lectin-mediated complement activation (Fujita, 2002).…”