Antimicrobial Activity Test of Mangosteen Leaves Ethanol Extract (Garcinia mangostana Linn) Against Pseudomonas aeruginosa Bacteria

Abstract

Pseudomonas aeruginosa causes various infections such as skin infections. Mangosteen leaves contains active compounds that are useful in inhibiting bacterial growth. The purpose of this research is to examine the antibacterial activity of mangosteen leaves ethanol extract (Garcinia mangostana Linn) on the growth of Ps. aeruginosa bacteria. The experiment was conducted by an experimental method of Ps. aeruginosa bacteria using the Kirby-Bauer method. The concentration of ethanol extract from mangosteen leaves studied is 10% to 100% concentration with a bacterial density of 0.5 Mc Farland. The results showed that the ethanol extract of mangosteen leaves could inhibit bacteria from a concentration of 10% with an average inhibitory diameter is 13.20 mm, 20% is 14.00 mm, 30% is 14.65 mm, 40% is 15.85 mm, 50% is 16.05 mm, 60% is 16.90 mm, 70% is 17.55 mm, 80% is 18.75 mm, 90% is 19.25 mm and 100% equal to 24.80 mm. Based on the results of research and analysis date that has been carried out, it can be concluded that the ethanol extract of mangosteen leaves (Garcinia mangostana Linn) has antibacterial activity against Ps. aeruginosa, with a minimum inhibitory concentration of 10% with diameter inhibitor zone is 13.20 mm.

Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of mangosteen (Garcinia mangostana Linn.) rind extract against Aggregatibacter actinomycetemcomitans

“…Mangosteen rind extract has an antibacterial activity which is similar to the research conducted by Suhartati et al 16 , which is suggested that the antibacterial effect of mangosteen rind extract is having a difference on the gram-negative and anaerobic bacteria. Phytochemical screening test of mangosteen rind showed that the ethanol extract of mangosteen rind contained active compounds, such as chrysanthemin, garcinone A, B, and C, sesquiterpenoids, gartanin, fructose, sucrose, tannins, and xanthone as the main content of mangosteen rind.…”

“…Permeability disruption of bacterial cells causes their growth is stunted or dead. 5,16 This study was still in vitro tests; therefore, it did not reflect the real condition found in periodontal pockets. It did not consider the factors such as the interaction of host, agent, and environment.…”

“…Also, xanthones are capable of coagulating proteins that cause the bacterial cell membrane to become lysis. 5,16,18 Tannins will coagulate bacterial cells and eliminate toxins. Tannins can shrink and interfere with the permeability of bacterial cell membrane.…”

Analysis of The Phytochemical Characteristics of Crude Extract of Jasmine Flower Against Escherichia coli and Staphylococcus aureus

“…According to suhartati et al, (2019) Flavonoids can perform inhibition mechanism by interfering with the bacterial cell wall synthesis there by causing plasma leakage resulting in lysis of the bacteria . Inhibition occurs also (Suhartati 2019) et al there is a process of protein synthesis, inhibition of protein synthesis occurs where there is a process of transcription and translation of genetic material so that the amino acids produced incorrectly place themselves in the peptide chain and produce proteins that do not function . Flavonoid compounds (Madigan 2008) et al can damage cell walls, causing cell death (Haeria and Hermawati 2016).…”

Natural Products Isolated from Various Parts of Mangosteen (Garcinia mangostana L.) as Therapeutic Agent: A Review

“…Moreover, the extract of hot water derived from mangosteen peel demonstrated epicatechin as the main component. Furthermore, based on Suhartati et al ( 2018), mangosteen leaves demonstrated four active compounds, such as tannins, flavonoids, alkaloids, and saponins [12][13][14].…”