Antimicrobial activity of photodynamic therapy in combination with colistin against a pan-drug resistant Acinetobacter baumannii isolated from burn patient

Boluki, Ebrahim; Kazemian, Hossein; Peeridogaheh, Hadi; Alikhani, Mohammad Yousef; Shahabi, Sima; Beytollahi, Leili; Ghorbanzadeh, Roghayeh

doi:10.1016/j.pdpdt.2017.01.003

Cited by 46 publications

(35 citation statements)

References 29 publications

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“…A similar result was also obtained with the combined use of aPDT and norfloxacin. Another interesting application of combining aPDT and antibiotic treatment was described by Boluki et al [38]. They used aPDT and CL against pan-drug-resistant Acinetobacter baumannii strain.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic enhancement of the activity of antibiotics used in urinary tract infections

et al. 2019

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Photodynamic therapy (PDT) has been proven to kill different microbial cells. However, to our knowledge, none of the available reports describes the modulatory effect of this therapy on the antibacterial activity of antibiotics against Escherichia coli rods being the main causative agent of urinary tract infections (UTIs). Therefore, the aim of our study was to verify if the PDT can enhance the antibacterial activity of antibiotics recommended in the treatment of UTIs. An attempt to determine the optimal conditions of PDT to enhance the bactericidal activity of ciprofloxacin, amikacin, and colistin has been made. In order to find the optimal antimicrobial conditions, the efficacy of four protocols associated with the use of different energy doses (70 and 120 J/ cm 2 ) and chlorin e6 (Ce6) concentrations (50 and 100 μg/mL) has been verified. The antibacterial effect of combined PDT and antibiotics was assessed by the time-kill assay. The best results were achieved for Ce6 at a concentration of 100 μg/mL and the energy dose 120 J/cm 2 for bacterial suspensions treated with ciprofloxacin. Taken together, our results showed that PDT using Ce6 improves the antibacterial activity of antibiotics effectively inhibiting bacterial growth and being promising in the elimination of bacterial UTIs in humans.

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Section: Discussionmentioning

confidence: 99%

Photodynamic enhancement of the activity of antibiotics used in urinary tract infections

et al. 2019

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“…Although after calculating sublethal effects, the expressions of Pmr A-Pmr B genes were evaluated through real-time PCR method, it was indicated that rates were reduced in 6.1 to 4.1 fold. 35 The efficacy of photodynamic therapy on the formation of biofilm P. gingivalis was evaluated by Pourhajibagher et al using the inducianin green photosensitizer. The results showed that indosanine green at a concentration of 62.5-1000 μg/mL reduced a significant number of bacteria, which decreased compared to the control group (23.2%-93.7%).…”

Section: Application Of Photodynamic Therapy In Vitromentioning

confidence: 99%

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

et al. 2018

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Introduction: The purpose of this review was to evaluate the available literature for in vitro and in vivo effectiveness of antimicrobial Photodynamic therapy (aPDT) in the field of bacteriology. Methods: A review of the relevant articles carried out in PubMed and Scopus to determine the efficiency of aPDT used in the reduction of microbial infection. Thirty-one relevant documents retrieved from PubMed, Scopus by inserting "antimicrobial photodynamic therapy" and "bacterial infection" and "photodynamic therapy" keywords. Results: According to different results, aPDT can be used as an adjuvant for the treatment of infectious diseases. The use of photosensitizer methylene blue, toluidine blue O (TBO), indocyanine green with light diode laser centered at (630±10 nm) and (650±10 nm) wavelengths have been shown to have significant results for the treatment of infectious diseases and bactericidal properties Conclusion: These findings suggest that, aPDT can be an efficient method in the treatment of localized and superficial infections.

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“…65,66 According to previous studies, it has been proved that PDT procedure has destructive effects on the expression of particular systems in bacterial cells. For example, reduction of pmrA/ pmrB 2-component signal system after PDT occurred in one study, 67 and downregulation of biofilm-related genes (by conjugative PS) is another example of this effect. 68 Furthermore, there have been a number of experiments that have been worked on in preparation of a substrate which can be useful for another remedy.…”

Section: Photodynamic Therapy As a Procedures For Weakening Ta Systemsmentioning

confidence: 98%

Modulation of Toxin-Antitoxin System Rnl AB Type II in Phage-Resistant Gammaproteobacteria Surviving Photodynamic Treatment

et al. 2018

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Type II toxin-antitoxin (TA) systems are the particular type of TA modules which take part in different kinds of cellular actions, such as biofilm formation, persistence, stress endurance, defense of the bacterial cell against multiple phage attacks, plasmid maintenance, and programmed cell death in favor of bacterial population. Although several bioinformatics and Pet lab studies have already been conducted to understand the functionality of already discovered TA systems, still, more work in this area is required. Rnl AB type II TA module, which is composed of RnlA toxin and RnlB antitoxin, is a newly discovered type II TA module which takes part in the defense mechanism against T4 bacteriophage attack in Escherichia coli K-12 strain MH1 that has not been widely studied in other bacteria. Because of the significant role of class Gammaproteobacteriacea in a diverse range of health problems, we chose here to focus on this class to survey the presence of the Rnl AB TA module. For better categorization and description of the distribution of this module in this class of bacteria, the corresponding phylogenetic trees are illustrated here. Neighbor-joining and the maximum parsimony methods were used in this study to take a look at the distribution of domains present in RnlA and RnlB proteins, among members of Gammaproteobacteria. Also, the possible roles of photodynamic therapy (PDT) in providing a substrate for better phage therapy are herein discussed.

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Antimicrobial activity of photodynamic therapy in combination with colistin against a pan-drug resistant Acinetobacter baumannii isolated from burn patient

Cited by 46 publications

References 29 publications

Photodynamic enhancement of the activity of antibiotics used in urinary tract infections

Photodynamic enhancement of the activity of antibiotics used in urinary tract infections

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

Modulation of Toxin-Antitoxin System Rnl AB Type II in Phage-Resistant Gammaproteobacteria Surviving Photodynamic Treatment

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