Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

Mahmoudi, Hassan; Bahador, Abbas; Pourhajibagher, Maryam; Alikhani, Mohammad Yousef

doi:10.15171/jlms.2018.29

Cited by 90 publications

(44 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mechanism of photodynamic therapy depends on the local or systemic use of oxygen, combined with a photosensitizer (PS) and a visible light, producing reactive oxygen species (ROS) upon exposure. Oxygen species cause oxidative stress to ingredients of the bacterial cells such as membranes leading to complete biofilm elimination [193]. The unique advantage of this technique over other conventional ones is the dual selectivity behavior.…”

Section: Antimicrobial Photodynamic Therapy (Apdt)mentioning

confidence: 99%

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

et al. 2020

View full text Add to dashboard Cite

Based on the recent reports of World Health Organization, increased antibiotic resistance prevalence among bacteria represents the greatest challenge to human health. In addition, the poor solubility, stability, and side effects that lead to inefficiency of the current antibacterial therapy prompted the researchers to explore new innovative strategies to overcome such resilient microbes. Hence, novel antibiotic delivery systems are in high demand. Nanotechnology has attracted considerable interest due to their favored physicochemical properties, drug targeting efficiency, enhanced uptake, and biodistribution. The present review focuses on the recent applications of organic (liposomes, lipid-based nanoparticles, polymeric micelles, and polymeric nanoparticles), and inorganic (silver, silica, magnetic, zinc oxide (ZnO), cobalt, selenium, and cadmium) nanosystems in the domain of antibacterial delivery. We provide a concise description of the characteristics of each system that render it suitable as an antibacterial delivery agent. We also highlight the recent promising innovations used to overcome antibacterial resistance, including the use of lipid polymer nanoparticles, nonlamellar liquid crystalline nanoparticles, anti-microbial oligonucleotides, smart responsive materials, cationic peptides, and natural compounds. We further discuss the applications of antimicrobial photodynamic therapy, combination drug therapy, nano antibiotic strategy, and phage therapy, and their impact on evading antibacterial resistance. Finally, we report on the formulations that made their way towards clinical application.

show abstract

Section: Antimicrobial Photodynamic Therapy (Apdt)mentioning

confidence: 99%

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Це насамперед барвники з максимумами поглинання у червоній та зеленій областях видимого діапазону електромагнітних коливань. Ці ділянки спектру практично не виявляють патогенної дії на бактерій, але при додаванні у середовище фотосенсибілізатора, здатні викликати їх загибель [6]. Серед таких сполук найбільш відомим є метиленовий синій.…”

Section: вступunclassified

“…Після фотостимуляції фотосенсибілізатор переходить з синглетного у триплетний стан за механізмами міжсистемних переходів, який, у свою чергу, вступає у реакцію з навколишніми молекулами, генеруючи різні радикали та перекис водню або здатен передавати свою енергію на молекулярний кисень з утворенням його синглетного стану. Утворені вільні радикали та синглетний кисень взаємодіють з різними клітинними мішенями, в тому числі мембранами, нуклеїновими кислотами та ензиматичними комплексами, порушуючи їх функціонування [6].…”

Section: вступunclassified

See 1 more Smart Citation

Photoinactivation of staphylococcus aureus in vitro by red light (660 nm) in the presence of methylene blue

2019

Photobiology and Photomedicine

View full text Add to dashboard Cite

Introduction. Оpen wounds and ulcers treatment involves the use of bandage material, antibiotics and antiseptic to prevent the development of a pathogenic microflora and to provide the necessary conditions for tissue regeneration. An emergence of multi-resistant strains of microorganisms reduces the effectiveness of such technology and requires the new treatment approaches. One of the promising areas is a photodynamic therapy with the use of external photosensitizers. The aim of the investigation is to determine the effectiveness of the synergistic action of red light (660 nm) and different concentrations of methylene blue on the inhibition of Staphylococcus aureus growth. Materials and methods. We used the hydrogels based on the copolymers dextran-polyacrylamide with the different concentration of crosslinking agent 0.2 %, 0.4 %, 0.6 % (w/w) for investigation a rate of diffusion methylene blue into and out from hydrogel. Microbiological research was performed on wild strains of S. aureus isolated on a Yolk-salt agar. The evaluation of a bactericidal action of methylene blue was carried out on a Müller-Hinton No. 2 agar similarly to the disc-diffusion method for assessing the resistance of microorganisms to antibiotics. For irradiation by different wavelengths was used «LIKA-Led» (Photonics Plus) LEDs 390 nm, 460 nm and 660 nm. The radiation power for each wavelength was 100 mW, duration — 20 min, 30 min, 40 min. According to the duration, the irradiation doses were 21 J/cm2, 31.5 J/cm2, 42.1 J/cm2. Mathematical and statistical data processing was performed in the OriginLab 8.0 software package. Results. Increasing the amount of crosslinking agent in the hydrogel based on the copolymer dextran-polyacrylamide provides a decrease in the diffusion rate of methylene blue from the hydrogel. 390 nm ultraviolet radiation reduces the number of S. aureus colonies for 80% at 20 min exposure. Further increase in the exposure did not contribute to significant changes in this indicator. Blue light (460 nm) reduces the presence of this strain of microorganisms for 66% at 20 min exposure and reaches the effect of UV at 30 min exposure. Red light (660 nm) has no bactericidal effect. Minimal activity was found for methylene blue at concentrations of 0.001% and 0.0001% which was around 6 mm. The synergistic effect of 0.001% methylene blue and red light increases the activity for 40% up to 10 mm. Conclusions. For the photoinactivation of Staphylococcus aureus, it is advisable to use a low energy red light with a 660 nm wavelength in combination with a saturated methylene blue (0.001%) hydrogels. Perhaps the synergistic action of red light and dye provides a generation of active radicals that contribute to the growth retardation of microorganisms.

show abstract

“…In PDT, the excitation of a light sensitive drug (photosensitizer) by visible light in the presence of oxygen induces the formation of singlet oxygen via the transfer of intermolecular energy, which ultimately results in cytotoxicity ( Agostinis et al, 2011 ). PDT is currently being used as a cancer treatment and has recently shown promise for treating bacterial infections (reviewed in Dai et al, 2009 ; Cieplik et al, 2018 ; Mahmoudi et al, 2018 ). Antimicrobial PDT (aPDT) has several benefits as a therapeutic approach.…”

Section: Introductionmentioning

confidence: 99%

In vitro Photodynamic Therapy of Polymicrobial Biofilms Commonly Associated With Otitis Media

2020

View full text Add to dashboard Cite

Otitis media (OM) is a prevalent pediatric infection characterized by painful inflammation of the middle ear. There are more than 700 million cases of OM diagnosed globally each year, with 50% of affected children under 5 years of age. Further, OM is the most common reason for children to receive antibiotic treatment in developed countries. The most recent work on this dynamic disease indicates that biofilms and polymicrobial infections play a role in recurrent OM and chronic OM, which are difficult to eradicate using standard antibiotic protocols. Antimicrobial photodynamic therapy (aPDT) is a promising new strategy for the treatment of resistant bacteria and persistent biofilms which lead to chronic infections. While PDT continues to be successfully used for oncological, dermatological, and dental applications, our work focuses on the efficacy of aPDT as it relates to otopathogens responsible for OM. Previous studies from our laboratory and others have shown that non-typeable Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis , the three most common otopathogens, are susceptible to different forms of aPDT. However, many cases of OM involve multiple bacteria and to date no one has investigated the efficacy of this technology on these complex polymicrobial biofilms. We treated polymicrobial biofilms of the three most common otopathogens with the photosensitizer Chlorin e6 (Ce6) and a continuous wave 405 ± 10 nm light emitted diode. Our data show significant bactericidal activity on polymicrobial biofilms associated with OM. These studies indicate that aPDT warrants further analysis as a possible treatment for OM and our results provide the foundation for future studies designed to identify the optimal aPDT parameters for polymicrobial biofilm-associated infections of the middle ear.

show abstract

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

Cited by 90 publications

References 45 publications

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

Photoinactivation of staphylococcus aureus in vitro by red light (660 nm) in the presence of methylene blue

In vitro Photodynamic Therapy of Polymicrobial Biofilms Commonly Associated With Otitis Media

Contact Info

Product

Resources

About