Antimicrobial activity of <italic>Anonna mucosa</italic> (Jacq.) grown <italic>in vivo</italic> and obtained by <italic>in vitro</italic>culture

Abstract

Brazilian flora includes numerous species of medicinal importance that can be used to develop new drugs. Plant tissue culture offers strategies for conservation and use of these species allowing continuous production of plants and bioactive substances. Annona mucosa has produced substances such as acetogenins and alkaloids that exhibit antimicrobial activities. The widespread use of antibiotics has led to an increase in multidrug-resistant bacteria, which represents a serious risk of infection. In view of this problem, the aim of this work was to evaluate the antibacterial potential of extracts of A. mucosa obtained by in vitro techniques and also cultured under in vivo conditions. Segments from seedlings were inoculated onto different culture media containing the auxin picloram and the cytokinin kinetin at different concentrations. The calluses obtained were used to produce cell suspension cultures. The materials were subjected to methanol extraction and subsequent fractionation in hexane and dichloromethane. The antimicrobial activity against 20 strains of clinical relevance was evaluated by the macrodilution method at minimum inhibitory and minimum bactericidal concentrations. The extracts showed selective antimicrobial activity, inhibiting the growth of Streptococcus pyogenes and Bacillus thuringiensis at different concentrations. The plant tissue culture methods produced plant materials with antibacterial properties, as well as in vivo grown plants. The antibacterial activity of material obtained through biotechnological procedures of A. mucosa is reported here for the first time.

Antibacterial activity of seed extracts of various species of the Annonaceae family cultivated in Colombia

“…Although not all extracts showed activity against the different strains evaluated in this study, it has been reported that extracts from species of the Annonaceae family can modulate drug resistance (Barboza et al, 2015). These results indicate that the extracts have good potential for therapeutic uses against some pathogens.…”

Actividad inhibitoria in vitro de extractos etanólicos de plantas del género Annona y Piper sobre promastigotes de Leishmania braziliensis

“…Annona mucosa es una especie vegetal que ya ha demostrado actividad antibacteriana in vitro, contra cepas de Streptococcus pyogenes y Bacillus thuringiensis (18) . Por otro lado, los extractos de hojas de Annona montana, también han mostrado efectos citotóxicos resaltantes ante líneas celulares como Hep-G2, HT-29, los cuales evidenciaron selectividad ante la presencia de células normales (19) .…”

Biometria e matéria seca de plântulas de biribá quando submetidas a diferentes substratos

“…A anonácea é uma dessas culturas produzidas, possuindo grande apreço no comércio internacional de devido ao seu vasto número de variedades de espécies, e também por se adaptarem sob condições edafoclimáticas adversas, beneficiando e facilitando os índices de produtividade em relação a comercialização de seus frutos (José, Pires & Freitas, 2014) Entre as espécies mais cultivadas dessa família, encontra-se a Annona mucosa Jacq., o biribazeiro, nativo de matas pluviais atlânticas e da Amazônia Ferreira et al (2010). O fruto é conhecido como biribá, anona pinha, fruta da condessa e biribá do Pará (Costa & Müller, 1995), rico em sais minerais e vitaminas, sendo considerado uma planta com grande aptidão para fins econômicos e ecológicos, a qual possui capacidade para produzir metabólicos de ação antimicrobiana, biopesticida e medicinal (De Souza Barboza, Ferreira, De Paula Rosa Ignacio & Albarello, 2015). Ainda, os frutos dessa espécie, em mês de safra, são frequentemente achados em feiras e mercados devido ao seu sabor adocicado, sendo também de grande aceite popular.…”