Antimicrobial Activity of<i>Syzygium zeylanicum</i>(L.) DC. and<i>Syzygium hemisphericum</i>(Walp.) Alston

Deepika, N.; Saranya, Jothiramshekar; Eganathan, Palanisami; Sujanapal, P.

doi:10.1080/22311866.2014.890065

Cited by 4 publications

(6 citation statements)

References 6 publications

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“…Several previous studies showed that S. zeylanicum was to have antimicrobial activity against P. aeruginosa, E. coli, P. vulgaris, S. aureus, K. pneumoniae, and Bacillus subtilis (Deepika et al 2014). The abundance of phenolic and flavonoid compounds in the S. zeylanicum contributes to its strong antimicrobial activity (Deepika et al 2014;Microbiol et al 2016;Palanisamy et al 2011;Shilpa and Krishnakumar 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The culture medium was extracted using ethyl acetate (1:1) three times. The ethyl acetate extract was separated from the culture medium by separating funnel and evaporated using a rotary evaporator (Deepika et al 2014;Habisukan et al 2021). The extract was concentrated using an oven at 40°C.…”

Section: Cultivation and Extractionmentioning

confidence: 99%

“…S. zeylanicum has been used by people in various parts of the world to treat infections caused by various pathogenic bacteria. This plant contains alkaloids, glycosides, phenolics, flavonoids, tannins, saponins, and steroids, with various biological activities, including as an antibacterial (Nomi et al 2012;Anoop and Bindu 2015;Bhanu and Sabu 2017;Deepika et al 2014;Mayasani et al 2019). Plants that have been used as ethnomedicine for the treatment of certain diseases are promising candidates to obtain good bioactive compounds from their endophytic fungi (Uzma et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

et al. 2021

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Abstract. Syarifah, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas 22: 4572-4582. The decoction of the root bark of Syzygium zeylanicum has been used as traditional medicine, such as for treating pathogenic bacterial infections. Endophytic fungi that live in medicinal plant tissues have a high species diversity and biological activities correlate with their host. Therefore, this study aimed to explore the diversity of endophytic fungi from the root bark of S. zeylanicum and to determine the antibacterial activity of endophytic fungi and their secondary metabolites. In this study, we isolate and identify the endophytic fungi from the root bark of S. zeylanicum, continued by screening their antibacterial activity against two Gram-negative bacteria (Escherichia coli InaCCB5 and Salmonella thypi ATCC1048 and two Gram-positive bacteria (Staphylococcus aureus InaCCB4 and Bacillus subtilis InaCCB1204) by the Kirby-Bauer method. The fungal extract with the highest antibacterial activity proceeded with the isolation and determination of the structure of their bioactive compounds. The isolates were morphologically identified. Isolates that showed strong antibacterial activity were identified by molecular identification. Isolation of bioactive compounds was carried out by chromatographic techniques and the determination of the structure of pure chemical compounds was performed by the spectroscopic analysis. In total, there were 8 isolates of endophytic fungi were obtained from the root bark of S. zeylanicum, namely SZR1 – SZR8. SZR2 isolate has the highest antibacterial activity. Molecular identification through phylogenetic analysis showed that SZR2 isolate had high similarity with Penicillium brefeldianum. Isolation of bioactive compounds from SZR2 produced compound 1 in the form of light yellow crystals which showed strong antibacterial activity against S. typhi, E. coli, and B. subtilis with MIC values of 64 g/mL. Compound 1 was identified as p-hydroxybenzaldehyde, which was also obtained in its host. In conclusion, the endophytic fungus Penicillium brefeldianum produces similar secondary metabolites and antibacterial activity as its host plant.

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Section: Discussionmentioning

confidence: 99%

Section: Cultivation and Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

et al. 2021

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“…Endophytic fungi internally colonize plant tissues and have the potential to act as control agents, such as biologic agents or elicitors in resistance induction processes and in attenuating abiotic stresses [1]. The potential of the Syzygium zeylanicum plant as an antibacterial was described in the study of [4] here the leaf extract had a Minimum Inhibitory Concentration (MIC) value of 350 g/mL for E.coli and and P. aeruginosa, 1,000 g/mL for P. pneumonia and 500 g/mL for B. subtilis.…”

Section: Introductionmentioning

confidence: 99%

Active Compound Phenyl Ether from Potential Endophytic Fungi of Syzygium zeylanicum

et al. 2023

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Endophytic fungi can be found in various types of plants, especially medicinal plants, such as Syzygium zeylanicum, one of the many medicinal plants found in Indonesia. This study aimed to obtain isolates of endophytic fungi from the bark of Syzygium zeylanicum, L., with antibacterial and antioxidant activities and identify their bioactive compounds. Characterization of endophytic fungi was carried out morphologically and fungi that showed high bioactivity were characterized molecularly. Antibacterial activity was carried out by disc diffusion method and antioxidant activity by DPPH method. Isolation of bioactive compounds was carried out by chromatographic techniques and determination of the structure of pure chemical compounds by spectroscopic analysis. In this study, 3 isolates of endophytic fungi were obtained. Based on their morphological characterization, the endophytic fungi belong to the genera Trichoderma (Code SZT2 and SZT6), and Sclerotium (Code SZT8). Trichoderma mushroom extract (SZT2) gave high antibacterial activity (74.0 % against E. coli; 78.2 % against S. aureus; 80.5 % against S. tyhpi and 74.0 % against B. subtilis). The antioxidant activity was very strong SZT2 (IC50 = 5.83 µg/mL) SZT2 potential endophytic fungus based on molecular analysis is Trichoderma koningi. SZT2 mushroom extract has an active compound in the form of compound 3-hydroxy-4(hydroxy(4-hydroxyphenyl)methyl)-ɣ-butyrolactone based on the results of compound isolation using NMR and HMR. These endophytic fungal compounds can be developed into new sources of antibacterial and antioxidant bioactive compounds through a series of further studies. HIGHLIGHTS New movement in the discovery of antioxidant and antibacterial compounds with endophytic fungal sources. in this study we found compound A which have very strong antioxidant activity accompanied by strong antibacterial activity against 4 pathogenic bacteria. GRAPHICAL ABSTRACT

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“…Leaves of S. zeylanicum plant have been used by people in Indonesia as a medicinal plant related to pathogenic bacterial infections and the e ects of free radicals in the body (Anoop and Bindu, 2014;Anoop and Bindu, 2015;Deepika et al, 2014;Vinodkumar, 2015). Endophytes, especially those that inhabit medicinal plant tissues, are often used as sources of bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antibacterial Activities of Endophytic Fungi Extracts of Syzygium zeylanicum

Syarifah

Elfita

Widjajanti

et al. 2022

Sci. Technol. Indones

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Syzygium zeylanicum, one of the other therapeutic plants found in Indonesia, is used to treat various ailments, such as antimicrobial, anti-inflammatory, antioxidant, arthritis, antidiabetic, mosquitocidal, antitumor, and anti-rheumatic agents. The massive use of plant extracts has caused the development of isolation of bioactive compounds thru their endophytic. The present research aimed to obtain endophytic fungal isolates from the stem bark and leaves of Jambu nasi-nasi (S. zeylanicum) and analyze endophytic fungal extracts’ antioxidant and antibacterial activity. Endophyte identification was performed morphologically, and isolates with high biological activity were molecularly characterized. The antibacterial activity was evaluated by the disc diffusion method, and the antioxidant activity was evaluated by the DPPH method. In total, 10 endophytic fungi were isolated and identified as Phialemonium sp. (Code SZT3), Acremonium sp. (Code SZT4), Trichoderma aureoviridae (Code SZT5), Trichoderma koningi (Code SZT7), Phytium torulosum (Code SZL1), Phytium zingiberum (Code SZL2), Septonema sp. (Code SZL3), Lasiodiplodia pseudotheobromae (Code SZL4), Volutella cilliata (SZL5), and Trichophyton mentagrophytes (Code SZL7). Isolate SZL4 gave activity the highest antioxidant (IC50 = 3.30 ????g/mL) and strong antibacterial activity against four pathogens bacterial (S. thypi, B. subtilis, S. aureus, and E. coli). The potential endophytic fungi based on molecular analysis was Lasiodiplodia pseudotheobromae with accession number OK668257. These endophytic fungi can be developed into new sources of antibacterial and antioxidant bioactive compounds through further studies.

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Antimicrobial Activity ofSyzygium zeylanicum(L.) DC. andSyzygium hemisphericum(Walp.) Alston

Cited by 4 publications

References 6 publications

Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

Active Compound Phenyl Ether from Potential Endophytic Fungi of Syzygium zeylanicum

Antioxidant and Antibacterial Activities of Endophytic Fungi Extracts of Syzygium zeylanicum

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