Antimalarial Activity of Physalins B, D, F, and G

Sá, Matheus Santos de; Menezes, Melody; Krettli, Antoniana U.; Ribeiro, Ícaro José Santos; Tomassini, Therezinha Coelho Barbosa; Santos, Ricardo Ribeiro dos; Azevedo, Walter Filgueira de; Soares, Milena Botelho Pereira

doi:10.1021/np200260f

Cited by 78 publications

(35 citation statements)

References 26 publications

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“…Extracts and physalins obtained from P. angulata have shown a vast range of biological properties [10, 11, 14–16, 20, 21]. However, to date, no reports have described the morphological alterations in parasites of Leishmania genus in response to AEPa treatment.…”

Section: Resultsmentioning

confidence: 99%

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In vitro biological action of aqueous extract from roots of Physalis angulata against Leishmania (Leishmania) amazonensis

Silva

Rodrigues

et al. 2015

BMC Complement Altern Med

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BackgroundLeishmaniasis is an infectious disease caused by various species of the protozoan parasites of the Leishmania genus and transmitted by phlebotomine sandflies. The protozoa multiply in phagocytic cells, mainly macrophages, which play an important role defending the organism from pathogens. The most effective treatment for leishmaniasis is the chemotherapy and besides the high cost, these drugs are toxic and require a long period of treatment. Currently, some herbal products are considered an important alternative source of a new leishmanicidal agent, which includes the plant Physalis angulata, . We evaluated effects of an aqueous extract from roots of Physalis angulata (AEPa) on Leishmania proliferation, morphology and also determined whether physalins were present in the extract contributing to the knowledge of its pharmacological efficacy.MethodsMorphological alterations were determined by light microscopy, transmission and scanning electron microscopy. Host cell viability was evaluated by MTT, and propidium iodide. AEPa were submitted in full HRESITOF analysis.ResultsAEPa promoted a dose-dependent reduction on promastigotes (IC50 = 39.5 μg/mL ± 5.1) and amastigotes (IC50 = 43.4 μg/mL ± 10.1) growth. This growth inhibition was associated with several morphological alterations observed in promastigote forms. No cytotoxic effect in mammalian cells was detected (IC50 > 4000 μg/mL). Furthemore, the presence of physalins A, B, D, E, F, G and H were described, for the first time, in the P. angulata root.ConclusionsResults demonstrate that AEPa effectively promotes antileishmanial activity with several important morphological alterations and has no cytotoxic effects on host cells.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-015-0717-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…In addition, antiprotozoal effect of physalins was also been reported against Plasmodium sp . [16] and Trypanosoma cruzi [17]. Nevertheless, to isolate enough physalins to observe the above-mentioned effects, researchers have used large quantities of P. angulata leaves and/or stems.…”

Section: Introductionmentioning

confidence: 99%

In vitro biological action of aqueous extract from roots of Physalis angulata against Leishmania (Leishmania) amazonensis

Silva

Rodrigues

et al. 2015

BMC Complement Altern Med

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“…species, primarily to identify and assess substances with possible medicinal value (Guimarães et al 2009, Sá et al 2011. However, just a few researches have investigated its cultivation or management, including Souza & Amorim (2009), who assessed the growth and development of the species under water deficit, and Cruz et al (2015), who evaluated the influence of phosphate fertilizer on its cultivation.…”

Section: Abstract Resumomentioning

confidence: 99%

Hydroponic cultivation of Physalis angulata L.: growth and production under nitrogen doses1

Leite

Tanan

Nascimento

et al. 2017

Pesqui. Agropecu. Trop.

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The Physalis angulata L. species has attracted interest due to the production of compounds with pharmacological activity and its potential for fruiticulture. Given that it is a fast-growing and highly productive species, determining the most adequate nitrogen (N) doses could contribute to higher crop yields. This study aimed at assessing the influence of N concentrations, in a hydroponic system, on the growth and production of P. angulata, as well as determining the critical N level in leaves. The experiment was conducted in individual pots with nutrient solutions, applying a completely randomized design and twelve replications, using five N doses (0 mg L-1, 56 mg L-1, 112 mg L-1, 168 mg L-1 and 224 mg L-1). Growth and production indices, amount of total N on leaves and stems and critical N levels were assessed. The increase of N doses in the nutrient solution influenced plant growth and fruit production, as well as the accumulation of total N in the leaves and stems. Based on the maximum economic yield, a dose of 162 mg L-1 of N is recommended for hydroponics, which provided a fruit yield of 7.27 g m-2 and critical total N level in leaves of 51.98 g kg-1.

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“…saude.gov.br/portal/arquivos/pdf/RENISUS_2010.pdf). Physalis angulata extracts were shown to exhibit intense antimalarial activity and potent immunomodulatory and anti-inflammatory activities (McKerrow et al 1993, Ridley 2002, Murata & Goldberg 2003, Newman & Cragg 2007, Sá et al 2011, Willcox 2011.…”

Section: Strategies Used To Discover New Antimalarial Drugs -A Searchmentioning

confidence: 99%

New approaches in antimalarial drug discovery and development: a review

Aguiar

Rocha

Souza

et al. 2012

Mem. Inst. Oswaldo Cruz

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Malaria remains a major world health problem following the emergence and spread of Plasmodium falciparum that is resistant to the majority of antimalarial drugs. This problem has since been aggravated by a decreased sensitivity of Plasmodium vivax to chloroquine. This review discusses strategies for evaluating the antimalarial activity of new compounds in vitro and in animal models ranging from conventional tests to the latest high-throughput screening technologies. Antimalarial discovery approaches include the following: the discovery of antimalarials from natural sources, chemical modifications of existing antimalarials, the development of hybrid compounds, testing of commercially available drugs that have been approved for human use for other diseases and molecular modelling using virtual screening technology and docking. Using these approaches, thousands of new drugs with known molecular specificity and active against P. falciparum have been selected. The inhibition of haemozoin formation in vitro, an indirect test that does not require P. falciparum cultures, has been described and this test is believed to improve antimalarial drug discovery. Clinical trials conducted with new funds from international agencies and the participation of several industries committed to the eradication of malaria should accelerate the discovery of drugs that are as effective as artemisinin derivatives, thus providing new hope for the control of malaria

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Antimalarial Activity of Physalins B, D, F, and G

Cited by 78 publications

References 26 publications

In vitro biological action of aqueous extract from roots of Physalis angulata against Leishmania (Leishmania) amazonensis

In vitro biological action of aqueous extract from roots of Physalis angulata against Leishmania (Leishmania) amazonensis

Hydroponic cultivation of Physalis angulata L.: growth and production under nitrogen doses1

New approaches in antimalarial drug discovery and development: a review

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