ANTIHYPERTENSIVE ACTIVITY OF EXTRACT AND FRACTIONS OF MATOA (POMETIA PINNATA J. R & G FORTS) LEAVES

Abstract

Objective: The purpose of this study was to determine antihypertensive activity of extract and fractions of matoa (Pometia pinnata) leaves.Methods: Matoa leaves were extracted by reflux, followed by evaporating using rotary evaporator. Hypertension was induced by 50 mg/kg bw. NaCl and 1.5 mg/kg bw. prednisone orally, every day as long as 28 days, then continued over the next 28 days in the therapy period. Male Sprague Dawley rats were divided into 12 groups which were hydrochlorothiazide (0.45 mg/kg bw.), control group hypertensive, control normal, matoa leaves extract (MLE) (with doses of 50 mg/kg bw., 100 mg/kg bw., and 150 mg/kg bw.), ethylacetate fraction (with doses of 4.35 mg/kg bw., 8.71 mg/kg bw., and 13.06 mg/kg bw.), and water fraction (with doses of 10 mg/kg bw., 21.88 mg/kg bw., and 32.82 mg/kg bw.). Measurement of systolic and diastolic blood pressure was done every weeks using direct tail-cuff of noninvasive method. Then histomorphology of muscle heart was performed at the end of this research.Results: Ethylacetate fraction of matoa leaves 13.08 mg/kg bw. and MLE 150 mg/kg bw. gave significant result in lowering blood pressure (p<0.05) on the 28 th day of therapy and showed an equal profile with hydrochlorothiazide (0.45 mg/kg bw). Histomorphological result of rat's muscle heart found collagen production was increased in NaCl-prednisone induced rats.Conclusions: Extract and fractions of P. pinnata leaves could decrease blood pressure of NaCl-prednisone induced hypertension rats, but this effect was not liniear with doses and they did not decrease the collagen production in cardiac myocardium compared to normal group.

The Effect of Antihypertensive of Tali’s Bamboo Shoot Ethanol Extract (Gigantochloa apus (Schult.&Schult.F.)) to Male White Rats Sprague Dawley

“…This maceration method is assumed effective to use to avoid damage to active substances that cannot stand of heating [11]. Prednisone and NaCl induction were used to increase blood pressure in rats significantly and constantly through its activation mechanism of Renin Angiotensin Aldosterone System (RAAS) and fluid retention [12], [13]. Blood pressure measurement was performed by Tail Cuff-method using a tool called CODA®.…”

Identification of Chemical Content and Antihypertensive Activity Test of Ethanol Extract from Tali Bamboo Shoot (Gigantochloa apus (Schult. & Schult.F.))

“…This method is used to determine systolic and diastolic blood pressure. Prednisone and NaCl induction method is used to increase blood pressure in rats, because it will increase blood pressure significantly and constantly through the activation mechanism of Renin AngiotensinAldosterone System (RAAS) and fluid retention [9], [10]. The choice of hydrochlorothiazide as a positive control is because the mechanism of action of the drug is the same as the phytopharmaca material tested.…”

Aktivitas Antihipertensi Ekstrak dan Fraksi-Fraksi Daun Avokad (Persea americana Mill) pada Tikus Jantan dengan Parameter Sistolik dan Diastolik

“…Maserat diambil menggunakan kertas saring whatman. Maserat ditampung dan ampas dipisahkan, setelah didapat hasil hitunglah rendemennya (Purwidyaningrum et al, 2017a). Proses tersebut dilakukan di Laboratorium Kimia Sekolah Tinggi Ilmu Farmasi Yayasan Pharmasi Semarang.…”