Antihyperglycemic effect of <i>Trigonella foenum-graecum</i> (Fenugreek) seed extract in alloxan-induced diabetic rats and its use in diabetes mellitus: a brief qualitative phytochemical and acute toxicity test on the extract.

Abstract

The effects of ethanol extract of Trigonella foenum-graecum (Fenugreek) seeds on the blood glucose levels in alloxan-induced diabetic rats at different doses (2g/kg, 1g/kg, 0.5g/kg and 0.1g/kg) were studied. The hypoglycemic effect of extract was compared with that of the standard antidiabetic drug (glimepiride, 4mg/kg) single dose. The extract showed significant activity against the diabetic state induced by alloxan but the intensity of hypoglycemic effect varied from dose to dose. The most effective dose recognized was 1g/kg but that is still lower than the standard antidiabetic drug. No acute toxicity was observed for ethanol extract of T. foenum-graecum seed when it was administered orally at high dose level (3 g/kg body weight), which is higher than effective antihyperglycemic dose, and closely observed for 24 hrs for any mortality and next 10 days for any delayed toxic effects on gross behavioral activities. Phytochemical group tests were also accomplished and presence of alkaloids, steroids and carbohydrates were recognized in the extract.

Preventive and curative effect of Trigonella foenum-graecum L. seeds in C57BL/6J models of type 2 diabetes induced by high-fat diet

“…Previous studies in alloxan or streptozotocin-induced diabetic rats, rabbits and normal mice or glucose-infused rats found similar effects on plasma glucose (Ajabnoor and Tilmisany, 1988;Ali et al, 1995;Khosla et al, 1995;Abdel-Barry et al, 1997;Zia et al, 2001;Vats et al, 2002;Vijayakumar et al, 2005;Gad et al, 2006;Hannan et al, 2007;Xue et al, 2007;Mowla et al, 2009;Moorthy et al, 2010a, b;Yadav et al, 2010;Ramadan et al, 2011).…”

“…Hypoglycaemic properties of TFG seeds and leaves have been demonstrated in alloxan or streptozotocin-induced diabetic rats (Khosla et al, 1995;Abdel-Barry et al, 1997;Broca et al, 1999;Raju et al, 2001;Vats et al, 2003;Gad et al, 2006;Hannan et al, 2007;Xue et al, 2007;Kannappan and Anuradha, 2009;Mowla et al, 2009;Yadav et al, 2010), dogs (Ribes et al, , 1986Valette et al, 1984;Broca et al, 1999), mice (Ajabnoor and Tilmisany, 1988;Handa et al, 2005;Vijayakumar et al, 2005), healthy mice or rats (Zia et al, 2001;Yadav et al, 2010), in healthy volunteers (Abdel-Barry et al, 2000), and in diabetic patients (Madar et al, 1988;Sharma et al, 1990Sharma et al, , 1996Raghuram et al, 1994;Gupta et al, 2001;Kassaian et al, 2009). TFG has been also tested in vitro (Broca et al, 1999;Al-Habori et al, 2001;Vijayakumar et al, 2005Vijayakumar et al, , 2010Gad et al, 2006;Srichamroen et al, 2009).…”

Effect of Centaurium erythraea Rafn, Artemisia herba-alba Asso and Trigonella foenum-graecum L. on liver fat accumulation in C57BL/6J mice with high-fat diet-induced type 2 diabetes

“…A. herba-alba Asso (AHA) is also used for the treatment of diabetes, for its antihyperglycemic (Al-Khazraji et al, 1993;Al-Shamaony et al, 1994;Hamza et al, 2010;Mansi et al, 2007;Marrif et al, 1995;Stefkov et al, 2014;Testekin et al, 2007) and hypoglycemic effect (Hamza et al, 2011). T. foenum-graecum L. (TFG) has been traditionally used in the treatment of diabetes but most models on which TFG was tested were experimentally induced by streptozotocin or alloxan, which results in models closer to type 1 diabetes than the type 2 diabetes that is the most prevalent in human adults (Abdel-Barry et al, 1997;Ajabnoor and Tilmisany, 1988;Broca et al, 1999;Hannan et al, 2007;Khosla et al, 1995;Mowla et al, 2009;Vats et al, 2003;Zia et al, 2001). We have tested TFG during development of high fat diet induced diabetes and in mice with established diabetes (Hamza et al, 2010(Hamza et al, , 2011.…”

A Novel Synergistic Galactomannan-Based Unit Dosage Form for Sustained Release of Acarbose

“…Postprandial blood glucose levels were determined for all the groups at regular time intervals (0, 1,2,4,7,9,11,24,28,32,36,48,52,56,60, and 72 h) in the following study and the results are compiled in Fig. 6.…”