Antihepatotoxic agent in micropropagated plantlets of Eclipta alba

França, Suzelei de Castro; Bertoni, Bianca Waléria; Pereira, Ana Maria Soares

doi:10.1007/bf00048138

Cited by 33 publications

(20 citation statements)

References 7 publications

(6 reference statements)

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“…MS medium supplemented with Kin promoted greater rooting than BA and 2iP. A similar result was observed by Franca et al (1995). It was difficult to isolate a single shoot with root from each culture passage because of the possibility of damaging roots.…”

Section: Resultssupporting

confidence: 72%

“…Eclipta alba is widely used in India as a cholagogue and deobstruent in hepatic enlargement, and used to treat jaundice and other ailments of the liver and gall bladder (Orning et al, 1980). Coumestan-type compounds, wedelolactone and dimethyl wedelolactone, were isolated as the main active principles of Eclipta alba, both constituents exhibiting antihepatotoxic activity (Wagner et al, 1986;Franca et al, 1995). In vivo tests proved that wedelolactone neutralizes the lethal and myotoxic activities of rattlesnake venom (Mors et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore it is important to develop an efficient micropropagation technique for Eclipta alba to rapidly disseminate superior clones once they are identified. There have been few reports to date on micropropagation in the genus using nodal explants (Franca et al, 1995;Zafar and Sagar, 1999;Borthakur et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Baskaran

Jayabalan

2005

In Vitro Cell. Dev. Biol. - Plant

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SummaryAn efficient rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Eclipta alba (Asteraceae) by enhanced axillary shoot proliferation in cotyledonary node segments was designed. The medium type, various carbon sources, plant growth regulators, and coconut water markedly influenced in vitro propagation of Eclipta alba. An in vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of benzyladenine (4.4 mM), kinetin (4.6 mM), 2-isopentenyladenine (4.9 mM), gibberellic acid (1.4 mM), 5% coconut water, and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of cotyledonary node segments on a similar medium enabled continuous production of healthy shoots with similar frequency. Rooting was highest (94.3%) on full strength MS medium containing 9.8 mM indolebutyric acid. Micropropagated plants established in garden soil, farmyard soil, and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics as well as floral features. These plants grew normally without showing any morphological variation.

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Section: Resultssupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

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An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Baskaran

Jayabalan

2005

In Vitro Cell. Dev. Biol. - Plant

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“…Pharmacological investigations have elucidated association of hepatoprotective property mainly due to two active constituents; wedelolactone and desmethylwedelolactone (Franca et al 1995).…”

mentioning

confidence: 99%

“…Although, there are several reports on in vitro plant regeneration of this medicinally important plant (Franca et al 1995, Borthakur et al 2000, Gawde and Paratkar 2004, Dhaka and Kothari 2005, Ray and Bhattacharya 2008, Singh et al 2012) but attempts have not been made to standardize nutrient requirement to improve regeneration. As, the nutrient requirement of the cultured tissue may vary according to different stages of explant growth and development so an understanding of optimal nutrient concentration in the culture medium could lead to increase growth and evoke efficient in vitro morphogenesis.…”

mentioning

confidence: 99%

Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

Bardar

Kaul

Kachhwaha

et al. 2015

Plant Tissue Cult. & Biotech.

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This study highlights the effect of different inorganic micronutrients like copper, cobalt, molybdenum, zinc, boron, iodine, iron and manganese in accelerating and amplifying in vitro shoot bud induction and proliferation of a medicinally important plant, Eclipta alba (L.) Hassk. Direct shoot bud induction was observed on MS fortified with Kn (2 mg/l). However, maximum number of shoots was achieved when GA3, 0.5 mg/l was added to induction medium along with 1μM copper sulphate (ten times the normal MS level). Optimization of nutrient level in the basal medium promoted maximum regeneration response from both shoot tips and nodal explants. Elongated shoots were rooted in MS supplemented with IBA, 1.0 mg/l. Healthy, green plantlets with well developed roots, flowered normally in the field. Genetic stability of micropropagated plantlets was evaluated using RAPD markers. The amplification products were monomorphic in micropropagated plantlets and similar to those of mother plant revealing the genetic uniformity of plantlets. The regeneration protocol is highly efficient and reproducible so would be useful for mass multiplication, ex situ conservation and genetic transformation of E. alba (L.) Hassk.

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