Antigenotoxicity studies in Drosophila melanogaster

Gräf, Ulrich; Abraham, Suresh K.; Guzmán-Rincón, Judith; Würgler, Friedrich E.

doi:10.1016/s0027-5107(97)00298-4

Cited by 107 publications

(67 citation statements)

References 32 publications

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“…The assay is capable of detecting several types of DNA mutations, for instance, point mutations, nucleotide deletions, DNA breaks, and failure of recombination (Graf, Abraham, Guzman‐Rincon, & Wurgler, 1998). As shown in Table 1, 20 mmol/L urethane statistically induced DNA mutations (total spot), which is in line with a formerly report (Laohavechvanich et al., 2006).…”

Section: Resultsmentioning

confidence: 99%

Anti‐inflammatory and anti‐insulin resistance activities of aqueous extract from Anoectochilus burmannicus

Budluang

Pitchakarn

Ting

et al. 2016

Food Science & Nutrition

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This study investigated biological activities including antioxidative stress, anti‐inflammation, and anti‐insulin resistance of Anoectochilus burmannicus aqueous extract (ABE). The results showed abilities of ABE to scavenging DPPH and ABTS free radicals in a dose‐dependent manner. Besides, ABE significantly reduced nitric oxide (NO) production in the lipopolysaccharide (LPS)‐treated RAW 264.7 via inhibition of mRNA and protein expressions of nitric oxide synthase (iNOS). The LPS‐induced mRNA expressions of cyclooxygenase‐2 (COX‐2) and interleukin 1β (IL‐1β) were suppressed by ABE. Moreover, ABE exerted anti‐insulin resistance activity as it significantly improved the glucose uptake in tumor necrosis factor (TNF)‐α treated 3T3‐L1 adipocytes. In addition, ABE at the concentration of up to 200 μg/mL was not toxic to human peripheral blood mononuclear cells (PBMCs) and did not induce mutations. Finally, the results of our study suggest the potential use of A. burmannicus as anti‐inflammatory, anti‐insulin resistance agents, or food supplement for prevention of chronic diseases.

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Section: Resultsmentioning

confidence: 99%

Anti‐inflammatory and anti‐insulin resistance activities of aqueous extract from Anoectochilus burmannicus

Budluang

Pitchakarn

Ting

et al. 2016

Food Science & Nutrition

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“…This one-generation test, which is very efficient and sensitive, is based on the ability of fruit flies to metabolize certain procarcinogens to their reactive metabolites and has been used to study the genotoxicity and antigenotoxicity of various natural compounds (Idaomar et al, 2002;Laohavechvanich et al, 2006;Silva et al, 2006;Fragiorge et al, 2007;Mezzoug et al, 2007;Tellez et al, 2007;Valadares et al, 2008). The wing SMART is based on the principle that a loss of heterozygosity leads to the expression of recessive marker genes in larval imaginal disk cells, thereby yielding clones of mutant cells that can be identified as mosaic spots on the wings (Graf et al, 1984(Graf et al, , 1998. These spots can be produced by mitotic recombination or mutations (deletions, point mutations, specific types of translocation, etc.).…”

Section: Introductionmentioning

confidence: 99%

“…These spots can be produced by mitotic recombination or mutations (deletions, point mutations, specific types of translocation, etc.). The analysis of two genotypes (one with structurally normal chromosomes and another with a multiply inverted balancer chromosome) allows the quantitative determination of the recombinogenic activity of genotoxic compounds (Graf et al, 1998;Spanó et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

et al. 2008

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Panax ginseng is one of the most widely prescribed herbal medicines for the treatment of cancer, diabetes, chronic inflammation, and neurodegenerative and cardiovascular diseases. Since the use of alternative medicines in combination with conventional therapy may increase the risk of unwanted interactions, we investigated the possible genotoxicity of a water-soluble form of the dry root of P. ginseng (2.5, 5.0 or 10.0 mg/mL) and its ability to protect against the genotoxicity of doxorubicin (DOX; 0.125 mg/mL) by using the Drosophila melanogaster wing somatic mutation and recombination test (SMART) with standard and high-bioactivation crosses of flies. Panax ginseng was not genotoxic at the concentrations tested, whereas DOX-induced genotoxicity in marker-heterozygous flies resulted mainly from mitotic recombination. At low concentrations, P. ginseng had antirecombinogenic activity that was independent of the concentration of extract used. Recombination events may promote cancer, but little is known about the ability of P. ginseng to inhibit such recombination or modulate DNA repair mechanisms.

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“…The fruit fly Drosophila melanogaster with its well development array of genotoxicity test systems has been used in a number of studies on the modulatory effects of various compounds, along with somatic mutation and recombination tests (SMART) have been employed (Graf et al, 1998;Kaya et al, 2002;Rizki et al, 2004;Sinigaglia et al, 2004;Romero-Jiménez et al, 2005;Costa and Nepomuceno, 2006). …”

Section: Introductionmentioning

confidence: 99%

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

2007

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In this study two different crosses involving the wing cell markers mwh and flr 3 (standard (ST) cross and high bioactivation (HB) cross, the latter being characterized by a high constitutive level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens) were used to investigate the modulatory effects of ascorbic acid (AA) combined with the antitumor agent doxorubicin (DXR) in Drosophila melanogaster. We observed that the two different concentrations of AA (50 or 100 mM) had no effect on spots frequencies, while DXR treatments (0.2 or 0.4 mM) gave positive results for all types of spots, when compared to negative control. For marker-heterozygous (MH) flies, a protective effect was observed with the lower concentration of AA (50 mM) that was able to statistically decrease the frequency of spots induced by DXR (0.2 mM), while an enhanced frequency of spots induced by DXR was observed with the higher concentration of AA (100 mM), when compared to DXR treatment (p < 0.05). These results suggest that AA may interfere with free radicals generated by DXR and with other possible reactive metabolites. The efficiency of AA in protecting the somatic cells of D. melanogaster against mutation and recombination induced by DXR is dependent on the dose used and the protection is directly related to the activity of cytochrome P450 enzymes.

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Antigenotoxicity studies in Drosophila melanogaster

Cited by 107 publications

References 32 publications

Anti‐inflammatory and anti‐insulin resistance activities of aqueous extract from Anoectochilus burmannicus

Anti‐inflammatory and anti‐insulin resistance activities of aqueous extract from Anoectochilus burmannicus

Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

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