Antigenotoxicity of coffee in the Drosophila assay for somatic mutation and recombination

Abraham, Suresh K.

doi:10.1093/mutage/9.4.383

Cited by 125 publications

(79 citation statements)

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“…It has been demonstrated by the wing spot test that the co-administration of coffee is effective in significantly reducing the frequencies of single and twin spots induced by cyclophosphamide, Ascorbic acid modulation of doxorubicin genotoxicity 451 diethylnitrosamine, mitomicyn C, procarbazine and urethane (Abraham, 1994) and that turmeric inhibited the genotoxic effect of urethane (El Hanss et al, 1999). Furthermore, sodium selenite has been shown to be antigenotoxic in combination with potassium dichromate (Rizki et al, 2001) and AA modulated the genotoxic action of several mutagens (Kaya et al, 2002), showing the suitability of this test system for mimicking the normal intake of substances.…”

Section: Discussionmentioning

confidence: 99%

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Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

2007

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In this study two different crosses involving the wing cell markers mwh and flr 3 (standard (ST) cross and high bioactivation (HB) cross, the latter being characterized by a high constitutive level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens) were used to investigate the modulatory effects of ascorbic acid (AA) combined with the antitumor agent doxorubicin (DXR) in Drosophila melanogaster. We observed that the two different concentrations of AA (50 or 100 mM) had no effect on spots frequencies, while DXR treatments (0.2 or 0.4 mM) gave positive results for all types of spots, when compared to negative control. For marker-heterozygous (MH) flies, a protective effect was observed with the lower concentration of AA (50 mM) that was able to statistically decrease the frequency of spots induced by DXR (0.2 mM), while an enhanced frequency of spots induced by DXR was observed with the higher concentration of AA (100 mM), when compared to DXR treatment (p < 0.05). These results suggest that AA may interfere with free radicals generated by DXR and with other possible reactive metabolites. The efficiency of AA in protecting the somatic cells of D. melanogaster against mutation and recombination induced by DXR is dependent on the dose used and the protection is directly related to the activity of cytochrome P450 enzymes.

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Section: Discussionmentioning

confidence: 99%

“…c Frequency of clone formation: clones/flies/48,800 cells (without size correction). d Calculated as [DXR alone -DXR + AA) / DXR alone] X 100, according to Abraham (1994). e Including rare flr 3 single spots.…”

Section: Discussionmentioning

confidence: 99%

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

2007

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“…The frequencies of clone induction per 10 5 cells were used to determine the recombinogenic activity based on the following parameters: mutation frequency (F M ) = frequency of clones in BH flies/frequency of clones in MH flies, recombination frequency (F R ) = 1-F M , frequency of the total number of spots (F T ) = total number of spots in MH flies (considering mwh and flr 3 spots)/number of flies, frequency of mutation = F T x F M and frequency of recombination = F T x F R (Santos et al, 1999;Sinigaglia et al, 2006). Based on the control-corrected spot frequencies per 10 5 cells the percentage of inhibition by P. ginseng was calculated as (DOX alone -P. ginseng plus DOX/DOX alone) x 100 (Abraham, 1994). Tables 1 and 2 show the wing SMART results for the chronic treatment of larvae with P. ginseng alone (2.5, 5.0 or 10.0 mg/mL) or in combination with DOX (0.125 mg/mL) using flies from ST and HB crosses, respectively.…”

Section: Data Evaluation and Statistical Analysismentioning

confidence: 99%

Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

et al. 2008

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Panax ginseng is one of the most widely prescribed herbal medicines for the treatment of cancer, diabetes, chronic inflammation, and neurodegenerative and cardiovascular diseases. Since the use of alternative medicines in combination with conventional therapy may increase the risk of unwanted interactions, we investigated the possible genotoxicity of a water-soluble form of the dry root of P. ginseng (2.5, 5.0 or 10.0 mg/mL) and its ability to protect against the genotoxicity of doxorubicin (DOX; 0.125 mg/mL) by using the Drosophila melanogaster wing somatic mutation and recombination test (SMART) with standard and high-bioactivation crosses of flies. Panax ginseng was not genotoxic at the concentrations tested, whereas DOX-induced genotoxicity in marker-heterozygous flies resulted mainly from mitotic recombination. At low concentrations, P. ginseng had antirecombinogenic activity that was independent of the concentration of extract used. Recombination events may promote cancer, but little is known about the ability of P. ginseng to inhibit such recombination or modulate DNA repair mechanisms.

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“…These values were then employed to estimate the contribution of recombination and mutation to the incidence of total mutant spots per fly in trans-heterozygous flies (Andrade et al, 2004). The inhibition percentages (IP) were calculated using the total number of spots per wing with the following formula (Abraham, 1994 …”

Section: Discussionmentioning

confidence: 99%

“…Based on the clone induction frequency per 10 5 cells, we compared the number of observed spots in MH and BH flies and quantified the relative contribution (%) of mutation and recombination to the total number of observed spots (Frei et al, 1992;Abraham, 1994). Comparing the clone induction frequency obtained with DXR alone between geno types, we observed that 15.14% of the mutant clones produced in ST flies were a result of mutation and 84.86% resulted from recombination.…”

Section: Smartmentioning

confidence: 99%

Assessment of toxic, genotoxic, antigenotoxic, and recombinogenic activities of Hymenaea courbaril (Fabaceae) in Drosophila melanogaster and mice

Vale¹,

Silva²,

Oliveira³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Hymenaea courbaril L., popularly known as jatobá, is a plant species that grows in the forests of South America. The species has been used for culinary purposes and in folk medicine to treat arthritis and inflammations. Due to the increasing use of this plant globally, the present study aimed to evaluate the toxic, genotoxic, recombinogenic, and antigenotoxic effects of H. courbaril sap (Hycs) using the mouse bone marrow micronucleus test and the somatic mutation and recombination test (SMART) in Drosophila melanogaster. To evaluate the aneugenic and clastogenic activities revealed by the micronucleus test, the animals were treated with 3 doses of Hycs (5, 10, and 15 mL/kg body weight). To evaluate the antianeugenic and anticlastogenic activities, the animals were simultaneously treated with Hycs and mitomycin C (4 mg/kg body weight). To assess the mutagenic and recombinogenic activities using SMART, 3-day-old larvae derived from standard and high bioactivation crosses were treated with 3 doses of Hycs (3.0, 1.5, and 0.3 mL) for approximately 48 h. To evaluate antimutagenic and antirecombinogenic Protective effects of jatobá in mice and D. melanogaster activities, larvae derived from both crosses were co-treated with 3 doses of Hycs (3.0, 1.5, and 0.3 mL) and doxorubicin (0.125 mg/ mL). The mouse bone marrow micronucleus test revealed that Hycs exhibited no cytotoxic, clastogenic and/or aneugenic effects, but did show anticytotoxic, anticlastogenic and/or antianeugenic activities. The SMART revealed no mutagenic or recombinogenic effects, but antimutagenic and antirecombinogenic activities were observed in somatic cells of D. melanogaster from both crosses.

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Antigenotoxicity of coffee in the Drosophila assay for somatic mutation and recombination

Cited by 125 publications

References 0 publications

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Assessment of toxic, genotoxic, antigenotoxic, and recombinogenic activities of Hymenaea courbaril (Fabaceae) in Drosophila melanogaster and mice

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