Assessment of toxic, genotoxic, antigenotoxic, and recombinogenic activities of Hymenaea courbaril (Fabaceae) in Drosophila melanogaster and mice

Abstract

ABSTRACT. Hymenaea courbaril L., popularly known as jatobá, is a plant species that grows in the forests of South America. The species has been used for culinary purposes and in folk medicine to treat arthritis and inflammations. Due to the increasing use of this plant globally, the present study aimed to evaluate the toxic, genotoxic, recombinogenic, and antigenotoxic effects of H. courbaril sap (Hycs) using the mouse bone marrow micronucleus test and the somatic mutation and recombination test (SMART) in Drosophila melanogaster. To evaluate the aneugenic and clastogenic activities revealed by the micronucleus test, the animals were treated with 3 doses of Hycs (5, 10, and 15 mL/kg body weight). To evaluate the antianeugenic and anticlastogenic activities, the animals were simultaneously treated with Hycs and mitomycin C (4 mg/kg body weight). To assess the mutagenic and recombinogenic activities using SMART, 3-day-old larvae derived from standard and high bioactivation crosses were treated with 3 doses of Hycs (3.0, 1.5, and 0.3 mL) for approximately 48 h. To evaluate antimutagenic and antirecombinogenic Protective effects of jatobá in mice and D. melanogaster activities, larvae derived from both crosses were co-treated with 3 doses of Hycs (3.0, 1.5, and 0.3 mL) and doxorubicin (0.125 mg/ mL). The mouse bone marrow micronucleus test revealed that Hycs exhibited no cytotoxic, clastogenic and/or aneugenic effects, but did show anticytotoxic, anticlastogenic and/or antianeugenic activities. The SMART revealed no mutagenic or recombinogenic effects, but antimutagenic and antirecombinogenic activities were observed in somatic cells of D. melanogaster from both crosses.

Medicinal Plants from Mexico, Central America, and the Caribbean Used as Immunostimulants

“…Recently, it was described that this plant induces hepatotoxicity [ 97 ]. On the contrary, Hymenaea courbaril was shown to lack genotoxic and mutagenic effects [ 98 ]. Toxicological studies are necessary to provide safety in the use of plant extracts and their compounds in clinical trials.…”

Adulteration and Contamination of Commercial Sap of Hymenaea Species

“…The bark of H. martiana showed antioxidant activity and the presence of phenol compounds [19], in addition to antinociceptive, antiedematogenic, anti-inflammatory, and analgesic properties [20], and antimicrobial agents acting against Cryptococcus neoformans , Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum canis [21]. H. courbaril (also known as jatobá), a substitute for H. stigonocarpa and H. martiana, is also widely used to treat wounds, bronchitis, and stomach diseases [22]. …”

>i<Scaffolds>/i< de biopolímeros e resina de jatobá para utilização em engenharia tecidual

“…Isso permitiria a obtenção de diferentes tipos de scaffolds, como por exemplo, para a regeneração dérmica ou óssea, dependendo da interação entre a resina e o biopolímero utilizado. Além disso, a resina de Jatobá possui atividades antibacteriana, antiinflamatória e anti-tumoral (GIRÓN et al, 2008;VALE et al, 2013). Assim, a confecção de scaffolds utilizando a mistura dos biopolímeros com adição de resina de Jatobá, que possuam as propriedades descritas seria de grande interesse, uma vez que em literatura não se tem registro de tais combinações.…”

“…Estudos com a espécie H. courbaril indicaram atoxicidade para células sadias e efeito antigenotóxico, que pode ser atribuído a uma atividade antioxidante de alguns de seus componentes (VALE et al, 2013). Seu óleo essencial que contém óxido de cariofileno e transcariofileno mostrou ação antifúngica para espécies de Candida .…”

“…É composta principalmente por diterpenos labdânicos e sesquiterpenos (RODRIGUES, 2017), os quais tem cargas negativas e, através da interação com as cargas dos materiais, auxiliariam na obtenção de scaffolds com morfologia diferenciada. A resina também pode conter flavonoides, lipídios, taninos, procianidinas e óleos essenciais (VALE et al, 2013). Os lábdanos presente na sua composição apresentaram atividades antibacteriana, anti-inflamatória e antitumoral (GIRÓN et al, 2008), já os flavonoides mostraram efeito citotóxico para células tumorais, efeito antigenotóxico na medula óssea e atividade antioxidante (SPERA et al, 2019).…”