“…Low amount of phenolic and flavonoid contents were estimated in the ethanol and methanol extracts, respectively. The result was in accordance with that of Dendrobium speciosum (Moretti et al 2013) and D. macraei (Prajapati and Patel 2013). Various compounds of bibenzyl, stilbenoids and Dendrobium other than D. amoenum (Fan et al 2009;Mukherjee et al 2012;Moretti et al 2013;Xing et al 2013).…”
Section: Discussionsupporting
confidence: 88%
“…The result was in accordance with that of Dendrobium speciosum (Moretti et al 2013) and D. macraei (Prajapati and Patel 2013). Various compounds of bibenzyl, stilbenoids and Dendrobium other than D. amoenum (Fan et al 2009;Mukherjee et al 2012;Moretti et al 2013;Xing et al 2013). Antioxidant activity of only isoamoenylin isolated from D. amoenum has been reported previously (Venkateswarlu et al 2002).…”
Dendrobium amoenum has biologically active phytoconstituents valued for traditional Chinese or folk medicine as tonic. Total phenolic and flavonoid contents of its stem extract was estimated by using Folin-Ciocalteu’s reagent and Aluminium chloride methods respectively. Antioxidant activity was determined by DPPH free radical scavenging method. Total phenolic content found in acetone (134.34 μg GAE/mg extract) and chloroform (101.55 μg GAE/mg extract) extract was significantly higher than other solvent extracts. Similarly, these two extracts had significantly high flavonoid content (acetone: 115.73 μg QE/mg extract, and chloroform: 84.16 μg QE/mg extract). Presence of high phenolic and flavonoid contents in these extracts showed the highest antioxidant activity. Highest antioxidant activity of these extracts was determined by their lowest IC50 value (acetone: 53.19 μg/ml and chloroform: 36.48 μg/ml). Significant negative relationship was found between phenolic content and IC50 (R2 = 0.209, p < 0.01) and flavonoid content and IC50 (R2 = 0.389, p < 0.01), which indicates high antioxidant activity due to high phenolic and flavonoid contents. This result revealed that D. amoenum act as an antioxidant agent due to its free radical scavenging activity which plays a crucial role in the development of new chemotherapeutic agents.
“…Low amount of phenolic and flavonoid contents were estimated in the ethanol and methanol extracts, respectively. The result was in accordance with that of Dendrobium speciosum (Moretti et al 2013) and D. macraei (Prajapati and Patel 2013). Various compounds of bibenzyl, stilbenoids and Dendrobium other than D. amoenum (Fan et al 2009;Mukherjee et al 2012;Moretti et al 2013;Xing et al 2013).…”
Section: Discussionsupporting
confidence: 88%
“…The result was in accordance with that of Dendrobium speciosum (Moretti et al 2013) and D. macraei (Prajapati and Patel 2013). Various compounds of bibenzyl, stilbenoids and Dendrobium other than D. amoenum (Fan et al 2009;Mukherjee et al 2012;Moretti et al 2013;Xing et al 2013). Antioxidant activity of only isoamoenylin isolated from D. amoenum has been reported previously (Venkateswarlu et al 2002).…”
Dendrobium amoenum has biologically active phytoconstituents valued for traditional Chinese or folk medicine as tonic. Total phenolic and flavonoid contents of its stem extract was estimated by using Folin-Ciocalteu’s reagent and Aluminium chloride methods respectively. Antioxidant activity was determined by DPPH free radical scavenging method. Total phenolic content found in acetone (134.34 μg GAE/mg extract) and chloroform (101.55 μg GAE/mg extract) extract was significantly higher than other solvent extracts. Similarly, these two extracts had significantly high flavonoid content (acetone: 115.73 μg QE/mg extract, and chloroform: 84.16 μg QE/mg extract). Presence of high phenolic and flavonoid contents in these extracts showed the highest antioxidant activity. Highest antioxidant activity of these extracts was determined by their lowest IC50 value (acetone: 53.19 μg/ml and chloroform: 36.48 μg/ml). Significant negative relationship was found between phenolic content and IC50 (R2 = 0.209, p < 0.01) and flavonoid content and IC50 (R2 = 0.389, p < 0.01), which indicates high antioxidant activity due to high phenolic and flavonoid contents. This result revealed that D. amoenum act as an antioxidant agent due to its free radical scavenging activity which plays a crucial role in the development of new chemotherapeutic agents.
“…[18][19][20] This is manifested by strong free radical scavenging activity; acetonic extract (DLA) superior with lowest IC 50 as similar with the standard ascorbic acid (AA). [21][22][23] The role of antioxidants is their interaction depends on the oxidative free radicals. The summary of DPPH method is that antioxidants react with the stable free radical, 2,2-diphenyl-1-picrylhydrazyl (deep violet colour) and convert it to 2,2-diphenyl-1-picrylhydrazine with discolouration.…”
Context: Dendrobium longicornu is a traditional medicinal plant widely used in Asia. It has many bioactive compounds like bibenzyl, phenanthrenes, phenolic compounds. There has been little research in the cytotoxic and antioxidant effects of D. longicornu. Aims: The aim of this study was to investigate the cytotoxic and antioxidant activities of this plant. Settings and Design: Antioxidant and cytotoxic activity of Dendrobium longicornu extracts. Methods and Material: The plant extracts were prepared by soxhlet's extractor in organic solvents, acetone and ethanol. The total polyphenol content (TPC) in the extracts was determined spectrophotometrically by the Folin-Ciocalteu method and the total flavonoid content (TFC) by aluminium chloride method. The antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The cytotoxic activity was evaluated against human brain tumor cells (U251) and cervical cancer cells (HeLa) using MTT assay. Statistical analysis used: Regression analysis was done for calculation of IC 50 . Duncan multiple range test and Dunnett test were done to compare the data. Results: The Dendrobium longicornu acetonic extract (DLA) showed significantly highest TPC and TFC than Dendrobium longicornu ethanolic extract (DLE). The antioxidant activity was also significantly higher in DLA followed by DLE. Highest cytotoxicity (i.e., lowest IC 50 value) was found for the DLA on U251 cells and DLE on HeLa cells. Conclusions: This result concluded that D. longicornu is a potential source of antioxidant and cytotoxic agents.
“…The comet assay was carried out basically following the original procedure [24], with minor modifications [27] using the double-spot system. Briefly, cell pellets were gently resuspended in 0.7% LMPA in PBS maintained at 37°C.…”
Lycium barbarum is a famous plant in the traditional Chinese medicine. The plant is known to have health-promoting bioactive components. The properties of Lycium barbarum berries cultivated in Umbria (Italy) and their effect on human hepatocellular carcinoma cells (HepG2) have been investigated in this work. The obtained results demonstrated that the Lycium barbarum berries from Umbria region display high antioxidant properties evaluated by total phenolic content and ORAC method, on hydrophilic and lipophilic fractions. Moreover, on HepG2 cell line Lycium barbarum berries extract did not change cell viability analyzed by MTT and Trypan blue exclusion assay and did not induce genotoxic effect analyzed by comet assay. Furthermore, it was demonstrated, for the first time, that the berries extract showed a protective effect on DNA damage, expressed as antigenotoxic activity in vitro. Finally, Lycium barbarum berries extract was able to modulate the expression of genes involved in oxidative stress, proliferation, apoptosis, and cancer. In particular, downexpression of genes involved in tumor migration and invasion (CCL5), in increased risk of metastasis and antiapoptotic signal (DUSP1), and in carcinogenesis (GPx-3 and PTGS1), together with overexpression of tumor suppressor gene (MT3), suggested that Umbrian Lycium barbarum berries could play a protective role against hepatocellular carcinoma.
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