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A field experiment was conducted at Institute of Agriculture and Animal Science (IAAS), Bhairahawa during winter season of 2019/2020 to find out the performance of wheat genotypes under timely sown (November 29, 2019) and late sown (December 25, 2019) conditions. The research was carried out in two environments (timely sown and late sown) following alpha lattice design with two replications. Each replication consists of five blocks with four plots in each block. The results revealed that all genotypes performance was decreased in late sowing environment. In late sown condition, there was significant difference among genotypes for all the traits under study and under timely sown condition, there was significant difference among genotype for all traits under study except weight of grains per spike and grain yield. The maximum yield was recorded in NL 1179 and Bhirkuti in timely and late sown conditions respectively. It was found that the mean grain yield in timely sown condition is 90.77 % higher than in late sown condition. The maximum grain yield was recorded in NL 1179 across combine environment. Int. J. Appl. Sci. Biotechnol. Vol 8(2): 161-169

Dendrobium amoenum has biologically active phytoconstituents valued for traditional Chinese or folk medicine as tonic. Total phenolic and flavonoid contents of its stem extract was estimated by using Folin-Ciocalteu’s reagent and Aluminium chloride methods respectively. Antioxidant activity was determined by DPPH free radical scavenging method. Total phenolic content found in acetone (134.34 μg GAE/mg extract) and chloroform (101.55 μg GAE/mg extract) extract was significantly higher than other solvent extracts. Similarly, these two extracts had significantly high flavonoid content (acetone: 115.73 μg QE/mg extract, and chloroform: 84.16 μg QE/mg extract). Presence of high phenolic and flavonoid contents in these extracts showed the highest antioxidant activity. Highest antioxidant activity of these extracts was determined by their lowest IC50 value (acetone: 53.19 μg/ml and chloroform: 36.48 μg/ml). Significant negative relationship was found between phenolic content and IC50 (R2 = 0.209, p < 0.01) and flavonoid content and IC50 (R2 = 0.389, p < 0.01), which indicates high antioxidant activity due to high phenolic and flavonoid contents. This result revealed that D. amoenum act as an antioxidant agent due to its free radical scavenging activity which plays a crucial role in the development of new chemotherapeutic agents.

Assessment of variability and traits association in crop help to enhance selection efficiency. Therefore, the present investigation entitled “Variability, Correlation and Path Coefficient Analysis for Agro-morphological Traits in Wheat Genotypes (Triticum aestivum L.) under Normal and Heat Stress Conditions” was carried out during winterseason of 2019/2020 at Institute of Agriculture and Animal Science (IAAS), Bhairahawa, Nepal to identify traits that highly contribute to grain yield and suitable for its further improvement. The experiment was laid out following alpha lattice design with two replications. The twentygenotypes of wheat was sown in two different environments viz., irrigated and heat stress in November 29, 2019 and December 25, 2019 respectively. It was found that under normal condition, moderate GCV and PCV were recorded in SW, TGW, NGPS, and WGPS. Under heat stress condition, high GCV and PCV were observed in GY. High heritability and high GAM was observed in TGW & GY, NGPS, WGPS, TGW under normal and heat stress condition respectively. Under normal condition SW exhibited positive correlation and high positive direct effect on GY at genetic level and WGPS at phenotypic level. And under heat stress condition SW exhibited positive correlation and high positive direct effect on GY. Whereas, at phenotypic level, WGPS and PH exhibited high positive direct effect on grain yield. Hence it is clear that spike weight and weight of grains per spike are important traits for grain yield improvement. Int. J. Appl. Sci. Biotechnol. Vol 9(1): 65-74

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