Cytotoxic Activity of Antioxidant-Riched Dendrobium longicornu

BMC Complement Altern Med

Chand

Pant

et al. 2018

Self Cite

BackgroundThe medicinal orchid Dendrobium moniliforme contains water-soluble polysaccharides, phenanthrenes, bibenzyl derivatives, and polyphenol compounds. This study explored the antioxidant and cytotoxic activities of D. moniliforme extracts and detected their bioactive compounds.MethodsPlant material was collected from the Daman of Makawanpur district in central Nepal. Plant extracts were prepared from stems using hexane, chloroform, acetone, ethanol and methanol. The total polyphenol content (TPC) in each extract was determined using Folin-Ciocalteu’s reagent and the total flavonoid content (TFC) in each extract was determined using the aluminium chloride method. The in vitro antioxidant and cytotoxic activities of each extract were determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays respectively. Gas chromatography and mass spectrometry (GC-MS) analysis was used to detect bioactive compounds.ResultsTPC content was highest (116.65 μg GAE/mg of extract) in D. moniliforme chloroform extract (DMC) and TFC content was highest (116.67 μg QE/mg of extract) in D. moniliforme acetone extract (DMA). D. moniliforme hexane extract (DMH) extract showed the highest percentage of DPPH radical scavenging activity (94.48%), followed closely by D. moniliforme ethanol extract (DME) (94.45%), DMA (93.71%) and DMC (94.35%) at 800 μg/ml concentration. The antioxidant capacities of DMC, DMA, DMH and DME, which were measured in IC50 values, were much lower 42.39 μg/ml, 49.56 μg/ml, 52.68 μg/ml, and 58.77 μg/ml respectively than the IC50 of D. moniliforme methanol extract (DMM) (223.15 μg/ml). DMM at the concentration of 800 μg/ml most inhibited the growth of HeLa cells (78.68%) and DME at the same concentration most inhibited the growth of U251 cells (51.95%). The cytotoxic capacity (IC50) of DMM against HeLa cells was 155.80 μg/ml of extract and that of DME against the U251 cells was 772.50 μg/ml of extract. A number of bioactive compounds were detected in both DME and DMM.ConclusionThe fact that plant extract of D. moniliforme has a number of bioactive compounds which showed antioxidant and cytotoxic activities suggests the potential pharmacological importance of this plant.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2197-6) contains supplementary material, which is available to authorized users.

Section: Discussionsupporting

confidence: 89%

Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS

BMC Complement Altern Med

Chand

Pant

et al. 2018

Self Cite

“…This indicates that the methanol extract is more potent on the cell lines used. The antitumor and anticancer activities of crude extracts of Dendrobium were explained in previous studies (Devi et al 2009;Lam et al 2015;Shrestha et al 2015;Sun et al 2016;Paudel et al 2017). In our previous study, we quantified the total phenolic and flavonoid contents in the crude extract of D. amoenum (Paudel et al 2015).…”

Section: Discussionmentioning

confidence: 77%

Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

Pant

2018

Botanica Orientalis

Self Cite

Dendrobium amoenum is an epiphytic orchid used as tonic because it has many derivatives of phenols. The crude extract of this orchid has been shown to have antioxidant activity. The objectives of this research are to explore the cytotoxic activity of antioxidant-rich crude extract against the human cervical carcinoma and glioblastoma cell lines by MTT assay and to detect the compounds by GC-MS. Methanol (DAM) extract of D. amoenum showed high cell growth inhibition percentage against the tested cell lines. DAM extract showed high cytotoxic activity against HeLa cells (IC50 – 110.22 μg/ml) and least activity against U-251 cells (IC50 – 550.55 μg/ml). Thirteen compounds were detected and identified in the extract. Based on abundance, four major compounds detected were: (E)-13-docosenoic acid; oleic acid; 11-octadecenoic acid, methyl ester; and hexadecanoic acid, 2,3-dihydroxypropyl ester. The cytotoxic activity of DAM extract is probably due to the presence of these bioactive compounds, confirmation of which needs further investigation. The result also highlighted the potential of this orchid as the source of natural anticancer drug and to explore their isolation, identification and characterization.Botanica Orientalis – Journal of Plant Science (2017) 11: 38–42

“…Dendrobium longicornu exhibits the DPPH free radical scavenging capacity as it has many antioxidant-rich secondary metabolites [ [4] , [5] , [6] , [7] , 29 ]. We have already evaluated the antioxidant, cytotoxic and antimicrobial capacity of its wild counterparts [ 8 , 9 ]. Its secondary metabolites scavenged DPPH radicals that would be measured at 517 nm.…”

Section: Discussionmentioning

confidence: 99%

“…The whole plant of D. longicornu largely contains bibenzyl and phenanthrene, as well as minimal amounts of monoaromatics, steroid and flavonoid derivatives [ [4] , [5] , [6] , [7] ]. Due to the presence of such compounds, its extract has been shown the antioxidant, antimicrobial and cytotoxic effects [ 8 , 9 ]. Its wild resource has been depleted by overexploitation to meet the demand in traditional medicine.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, anticancer and antimicrobial effects of In vitro developed protocorms of Dendrobium longicornu

Joshi

Chand

et al. 2020

Biotechnology Reports

Self Cite

Highlights In vitro protocorms development for Dendrobium longicornu was successfully established on endophytic elicitor supplemented MS-medium. Methanol extract of protocorms biomass was evaluated for its antioxidant, anticancer and antimicrobial effects. Bioactive secondary metabolites present in methanol extract were identified by GC–MS technique. Methanol extract showed moderate antioxidant, anticancer and antimicrobial effects. Protocorms of this plant are the good source of bioactive compounds for the industrial applications.