Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera

Hierholzer, John C.; Stone, Y O; Broderson, J. Roger

doi:10.1007/bf01316753

Cited by 89 publications

(59 citation statements)

References 64 publications

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“…The adenovirus and its types were identified using immunological assays, such as the hemagglutination inhibition and cell culture-based type-specific neutralization assays. Adenovirus type-specific sera are employed in both of these assays to identify the adenovirus type (8)(9)(10). Currently, the typing can be done through PCR amplification and sequencing of the hexon, penton, and fiber genes.…”

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

2016

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SUMMARY: Adenoviruses are responsible for approximately 5-10z of acute respiratory infections globally. However, there are a limited number of reports on the types of circulating respiratory human adenoviruses (HAdV) in India. We detected HAdV in the post-mortem specimens of a young child who died as a result of an acute febrile illness. To retrospectively investigate the circulating adenovirus types in the Alappuzha region, samples (n ＝ 235) collected from patients with influenza-like illnesses who participated in the influenza surveillance program were screened for HAdV. Fourteen samples were identified as positive for adenovirus by PCR analysis. Adenovirus was isolated from 3 of the 14 PCRpositive samples cultured using HEK-293 cell lines. The viral strains isolated in the study were from children between 6 and 10 years of age. The isolates were identified as adenovirus species C and E. Sequencing analysis of the fiber gene and a BLAST search revealed that 2 of the isolates were type HAdV-C2, and the third isolate was a HAdV-E4. A fiber gene sequence-based phylogenetic tree showed that the HAdV-E4 isolate was similar to the Japanese HAdV-E4 strain, whereas the HAdV-C2 isolates formed a distinct cluster. Respiratory infections due to HAdV-E4 are generally observed in adults; this study is the first to demonstrate the involvement of the HAdV-E4 strain in respiratory illnesses in children.

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Section: Introductionmentioning

confidence: 99%

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

2016

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“…Typing has usually been done by neutralization or hemagglutination inhibition (Rosen 1960, Hierholzer 1973, Hierholzer et al 1991. To circumvent practical problems associated with traditional serum neutralization studies molecular typing methods have been proposed (Kidd et al1996, Li et al 1999, Elnifro et al 2000, such as, restriction endonuclease analysis of full-length adenovirus DNA or by PCR using typespecific primers (Garnet et al 2002).…”

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Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection

Rosete

Manjarrez

Barrón

2008

Mem. Inst. Oswaldo Cruz

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“…An initial characterization of cell tropism showed that species D viruses had lower levels of luciferase expression in human lung epithelial cells as compared with the species C Ad5. This could be explained by the fact that Ad5 is a respiratory pathogen and is able to replicate efficiently in human lung A549 cells, whereas Ad26, Ad28, and Ad48 were all isolated from rectal swabs from a 9-month-old male, 30-month-old male, and an immunocompromised adult, respectively (Hierholzer et al, 1991;Schnurr and Dondero, 1993). Both species C and D viruses were capable of infecting human T lymphocytes; however, there was a high degree of variation in the species D viruses.…”

Section: Discussionmentioning

confidence: 99%

CD46-Mediated Transduction of a Species D Adenovirus Vaccine Improves Mucosal Vaccine Efficacy

et al. 2014

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The high levels of preexisting immunity against Adenovirus type 5 (Ad5) have deemed Ad5 unusable for translation as a human vaccine vector. Low seroprevalent alternative viral vectors may be less impacted by preexisting immunity, but they may also have significantly different phenotypes from that of Ad5. In this study we compare species D Ads (26, 28, and 48) to the species C Ad5. In vitro transduction studies show striking differences between the species C and D viruses. Most notably, Ad26 transduced human dendritic cells much more effectively than Ad5. In vivo imaging studies showed strikingly different transgene expression profiles. The Ad5 virus was superior to the species D viruses in BALB/c mice when delivered intramuscularly. However, the inverse was true when the viruses were delivered mucosally via the intranasal epithelia. Intramuscular transduction was restored in mice that ubiquitously expressed human CD46, the primary receptor for species D viruses. We analyzed both species C and D Ads for their ability to induce prophylactic immunity against influenza in the CD46 transgenic mouse model. Surprisingly, the species D vaccines again failed to induce greater levels of protective immunity as compared with the species C Ad5 when delivered intramuscularly. However, the species D Ad vaccine vector, Ad48, induced significantly greater protection as compared with Ad5 when delivered mucosally via the intranasal route in CD46 transgenic mice. These data shed light on the complexities between the species and types of Ad. Our findings indicate that more research will be required to identify the mechanisms that play a key role in the induction of protective immunity induced by species D Ad vaccines.

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Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera

Cited by 89 publications

References 64 publications

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection

CD46-Mediated Transduction of a Species D Adenovirus Vaccine Improves Mucosal Vaccine Efficacy

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