Antigen specificity of antibody responses to Corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis

Serology and clinical relevance of Corynebacterium pseudotuberculosis in native Korean goats (Capra hircus coreanae)

“…These findings are consistent with a previous study in which ELISA plates coated with recombinant phospholipase D were highly specific and sensitive for detecting C. pseudotuberculosis infection (Menzies et al 1994). Moreover, Ellis et al (1990) suggested that the recombinant antigen could be useful for overcoming problems with nonspecific cross-reaction in serological assays. Despite r e p o r t s o f c r o s s -r e a c t i o n w i t h M y c o b a c t e r i u m paratuberculosis (Pepin et al 1987) and of no significant relationship between the extent of CLA lesions and antibody titer (Ellis et al 1990), ELISA has been shown effective in assessing the eradication of CLA in sheep and goats (Dercksen et al 1996;Baird and Malone 2010).…”

“…Moreover, Ellis et al (1990) suggested that the recombinant antigen could be useful for overcoming problems with nonspecific cross-reaction in serological assays. Despite r e p o r t s o f c r o s s -r e a c t i o n w i t h M y c o b a c t e r i u m paratuberculosis (Pepin et al 1987) and of no significant relationship between the extent of CLA lesions and antibody titer (Ellis et al 1990), ELISA has been shown effective in assessing the eradication of CLA in sheep and goats (Dercksen et al 1996;Baird and Malone 2010). While only 6.3 % (29/461) of the tested goats presented CLA lesions, a h i g h s e r o p r e v a l e n c e o f 5 7 .…”

In vivo evaluation of antiseptics and disinfectants on control of Caseous Lymphadenitis: clinical, haematological, serological and microbiological monitoring

“…The inflammatory reaction caused by the irritant action of iodine tincture at 10% and of sodium hypochlorite at 2.5% might also have stimulated cell migration, with greater exposure to antigens and, consequently, increased humoral immunity. It is worth mentioning that immune activity triggered against C. pseudotuberculosis is complex and involves both humoral response as well as cell response (Ellis et al, 1990). Hence the increase in serum titers of animals does not necessarily imply protection against re-infection or relapse of clinical symptoms.…”

Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen

“…Consequently, the low level of specific antibodies against this enzyme in animals with no clinical signs of disease probably occurs because of the low production of exotoxin by the bacteria or poor humoral immune activation. The 70KDa antigen that was well recognized by both clinical and subclinical animals may be the 67KDa protein described by other authors (Sting et al 1998, Paule et al 2003, Moura-Costa et al 2008; it is classified as a heat shock protein (Ellis et al 1991).…”

“…But when the humoral recognition of these antigens by Western blotting was examined, sera from animals with clinical disease (presenting caseous lesions caused by C. pseudotuberculosis) recognized antigens with molecular weights of 32 and 70KDa, while sera from animals with no clinical symptoms of CLA but presenting positive serological results (subclinically infected animals), reacted strongly to the 70KDa antigen, but weakly recognized the 32KDa protein (Fig.2). It is highly probable that this 32KDa antigen corresponds to the 31.5KDa fraction that has been characterized as the major secreted antigen of C. pseudotuberculosis, Phospholipase D (PLD) (Ellis et al 1991, Costa et al 1998. Phospholipase D is an exotoxin responsible for the dissemination of bacteria in infected animals, and it is a virulence factor related to the formation of caseous lesions (Hodgson et al 1992).…”