In vivo evaluation of antiseptics and disinfectants on control of Caseous Lymphadenitis: clinical, haematological, serological and microbiological monitoring

Abstract

The objective of this experiment was to evaluate the efficacy of iodine tincture at 10% and sodium hypochlorite at 2.5% applied into the abscess of animals affected by Caseous Lymphadenitis (CL). Eighteen ewes were used, assorted into three groups: one treated with iodine tincture at 10% (IT), another one with sodium hypochlorite at 2.5% (SH) and the last group underwent the conventional treatment (CT). Conventional treatment was based on surgical drainage and chemical cauterization of the lesion with iodine tincture at 10%. Natural rupture of six abscesses from IT group was observed and in five of them the viability of Corynebacterium pseudotuberculosis was confirmed on the lesion place, after rupture of lymph node. As for the SH group, spontaneous rupture was observed in five out of six abscesses treated, and the microorganism was identified on the lesion of five animals, after rupture. In the sixth animal of this group, abscess involution was noticed. A severe swelling was identified in the region of lymph node treated, resulting in wide lesion in animals from groups IT and SH. No difference (p > 0,05) was found in blood parameters due to treatments. As for the serological monitoring of animals, comparative analysis between months within each group showed that months 1, 2, 3 and 4 were different (p < 0,05) from month 0, for groups IT and SH. For CT group, there was no difference (p > 0,05) between months 1 to 5 and 0. Then, the application of iodine tincture at 10% or sodium hypochlorite at 2.5% into the abscess of animals affected by CL, at stage in which lesions are detected through inspection, is not effective for its control.

Antimicrobial photodynamic therapy for caseous lymphadenitis abscesses in sheep: Report of ten cases

“…These authors also described adverse effects as a painful response induced by the use of 2.5% sodium hypochlorite and increased inflammatory reaction caused by the use of 10% iodine tincture. By the other hand, in our study APDT lead a complete cure in much less time and with no adverse effects than the treatments proposed by Santiago et al [5].…”

“…Different results were observed by Santiago et al [5], which studied the healing time of CLA abscesses using two antiseptic solutions: 10% iodine tincture and 2.5% sodium hypochlorite applied inside the abscesses and compared them with a surgical drainage of abscesses as a control group. The 10% iodine tincture group showed a mean time of 51.33 days for complete healing, followed by 2.5% sodium hypochlorite group with 52.5 days and the control group with 87.67 days.…”

Infecção por Corynebacterium pseudotuberculosis em equinos: aspectos microbiológicos, clínicos e preventivos

“…Mais recentemente, foi visto em um estudo que 196 amostras de C. pseudotuberculosis isoladas de equinos não tiveram grande variação em seus perfis de sensibilidade a antibióticos (Rhodes et al 2015). Mas deve-se levar em consideração também o fato de que, com a formação e desenvolvimento das lesões granulomatosas, o tratamento com antibióticos se torna um procedimento com baixa possibilidade de sucesso, pois a formação da cápsula do abscesso torna quase impossível a difusão dos antibióticos para a região da lesão em si, impedindo o contato da bacté-ria com o fármaco (Santiago et al 2013). …”

“…Esforços têm sido direcionados para evitar a abertura da lesão caseosa, através da inoculação de agentes antissépticos dentro do abscesso, mas esse procedimento ainda apresenta diversos efeitos colaterais aos animais, como já descrito para pequenos ruminantes (Santiago et al 2013). Também o acompanhamento sorológico frequente, através da detecção de anticorpos anti-C. pseudotuberculosis ou quantificação da produção específica de interferon-gama (Rebouças et al 2011;Rebouças et al 2013), como já descrito para caprinos e ovinos, pode ser um grande fator para evitar que mais animais dentro de um rebanho sejam infectados.…”

Biogenic Silver Nanoparticles as a Post-surgical Treatment for Corynebacterium pseudotuberculosis Infection in Small Ruminants

“…CL is characterized by the development of granulomas in the lymph nodes and in organs such as the spleen, lungs, liver, and kidneys (Dorella et al, 2006). The disease is associated with a decrease in the production of wool and meat, as well as a high cost of treatment (Santiago et al, 2013).…”