Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate

Ellis, John; Hawk, Douglas; Mills, Ken; Pratt, D.L.

doi:10.1016/0165-2427(91)90122-s

Cited by 23 publications

(18 citation statements)

References 20 publications

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“…These molecular masses may correspond to those of the phospholipase D exotoxin of C. pseudotuberculosis (Carne, 1940) and of the CP40 antigen previously described by Walker et al (1994). Most infected animals sera also recognised proteins of 26, 37, 63 and 67 kDa which were previously described (Ellis et al, 1991b;Braithwaite et al, 1993). It is noteworthy to stress on the high and persistent immunoreactivity of both 63 and 67 kDa antigens.…”

Section: Discussionsupporting

confidence: 68%

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Paule

Azevedo

Regis

et al. 2003

Veterinary Immunology and Immunopathology

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Corynebacterium pseudotuberculosis is the cause of caseous lymphadenitis (CLA) in small ruminants, a chronic granulomatous disease that provokes significant zootechnics losses to ovine and goat breeders in northern Brazil. The present work was conducted to analyse aspects of humoral and cellular immune responses after experimental infection. Eight goats were infected intradermally with a single dose of virulent C. pseudotuberculosis strain and specific IgG, interferon-gamma (IFN-gamma) production as well as IgG avidity and antigens pattern recognition dynamics against an excreted-secreted antigen were recorded during 20 weeks. At the end of the follow-up, animals were slaughtered and necropsied. Although no animals showed apparent clinical signs of infection at the end of the trial, IFN-gamma response, even more so than the humoral response, differentiated animals into two groups of high or medium/low response. The time-course of IFN-gamma production presented a short-lived primary response on day 5 after infection of animals of both groups, and a strong and long lasting secondary response starting on day 16 after infection in the high response group. The indirect ELISA used was able to detect a positive antibody titre between 6 and 11 days after infection in the two groups. IgG avidity index oscillated initially between 15 and 45%, and showed approximately 5% units increment during the 20 follow-up weeks. With only one individual exception, the qualitative antigens pattern recognition showed on day 11 after infection remained constant through the experiment. IgG avidity is highly correlated with IgG production, but could not be related with specific immunodominant bands. Both humoral and cellular responses kinetics presented a similar pattern of activation/deactivation but necropsy results suggested that the IFN-gamma test would be a very specific marker of CLA status.

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Section: Discussionsupporting

confidence: 68%

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Paule

Azevedo

Regis

et al. 2003

Veterinary Immunology and Immunopathology

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“…Este fato sugere que este fator de virulência contra o qual muitos trabalhos de proteção vacinal têm sido elaborados (Ellis et al 1991, Tachedjian et al 1995, Hodgson et al 1999, mantém uma homogeneidade nas suas características genéticas, permitindo assim que a resposta imune desenvolvida contra a PLD possa ser explorada como instrumento de controle da doença também na região estudada.…”

Section: Resultsunclassified

Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco

Abreu¹,

Mota

Rosinha

et al. 2008

Pesq. Vet. Bras.

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Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região.

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“…Therefore, attempts to control CLA usually entail immunoprophylaxis by vaccination. Various strategies have been used for developing vaccines against CLA, including the use of inactivated or attenuated C. pseudotuberculosis strains [4,5], fractions of bacterial cells containing bacterial antigens, antigens from culture supernatants, and DNA vaccines [6]. None of the currently commercially available vaccines for C. pseudotuberculosis provide effective protection against CLA [7].…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice

et al. 2011

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BackgroundHeat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants.FindingsTo construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60).ConclusionThis vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity.

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Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate

Cited by 23 publications

References 20 publications

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco

Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice

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