Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice

Abstract

BackgroundHeat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants.FindingsTo construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60).ConclusionThis vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity.

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

“…Hsp60 and Hsp10 form a folding cage through their rings and produce large and efficient protein-editing machinery that facilitates proper folding and assembly of mitochondrialimported proteins and corrects misfolded polypeptides (Hansen et al 2003;Magen et al 2008). Although the Hsp60/Hsp10 complex system has been well studied in many species; however, the regulatory mechanism of this system, especially in crustaceans, remains unclear (Costa et al 2011;Johnson et al 2014;Nisemblat et al 2014). The chaperon folding cage is composed of two parts: a dome-shaped heptameric ring of Hsp10 (GroES) subunits and Hsp60 (GroEL) cylinder with ATPase (Saibil and Ranson 2002).…”

Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

“…Despite the economic [1, 2] and zoonotic [3] relevance of CL, a satisfactory vaccine model has not been developed [4, 5]. …”

Co‐expression of S. Typhi GroEL and IL‐22 gene augments immune responses against Salmonella infection

“…Other advantages of DNA vaccine over conventional vaccines include no risk of infection as they are not likely to have any residual virulence associated with live attenuated strains, induction of long‐lived immune response, stability at low and high temperatures, easy preparation and low cost 41 , 42 , 43 . Direct Intramuscular injection of plasmid DNA encoding HSP60 of C. pseudotuberculosis has been reported to produce immune responses in mice 44 . Despite their multiple advantages, there are certain limitations of plasmid DNA vaccines.…”

“…[41][42][43] Direct Intramuscular injection of plasmid DNA encoding HSP60 of C. pseudotuberculosis has been reported to produce immune responses in mice. 44 Despite their multiple advantages, there are certain limitations of plasmid DNA vaccines. Studies have revealed that antibody levels achieved using DNA vaccines are lower than conventional vaccines.…”