Antigen Detection Assay for Identification of<i>Penicillium marneffei</i>Infection

Chaiyaroj, Sansanee C.; Chawengkirttikul, Runglawan; Sirisinha, Stitaya; Watkins, P. H.; Srinoulprasert, Yuttana

doi:10.1128/jcm.41.1.432-434.2003

Cited by 33 publications

(11 citation statements)

References 23 publications

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“…Detection of capsular polysaccharide by latex agglutination (LA) using a variety of U.S. Food & Drug Administration (FDA)‐cleared test kits is a most useful method for rapid diagnosis of cryptococcal meningitis, positive in about 90% of cases (Table 3) (48–59). While an FDA‐cleared EIA kit is available, it is more expensive than LA and offers no clear advantage (48).…”

Section: Cryptococcosismentioning

confidence: 99%

“…No cross‐reactions were seen with other fungal antigens including Histoplasma . In a subsequent publication sensitivity for detection of antigen in 1:10 diluted serum specimens was 92% and specificity 98% (57). Of note is that antigenemia in patients with penicilliosis marneffei causes a positive result in the Aspergillus galactomannan assay.…”

Section: Penicilliosis Marneffeimentioning

confidence: 99%

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Antigen detection, serology, and molecular diagnosis of invasive mycoses in the immunocompromised host

Wheat¹

2006

Transplant Infectious Dis

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Understanding the uses and limitations of methods for rapid diagnosis of fungal disease is essential in order to diagnose and treat these infections early in their course. Antigen detection methods are useful for diagnosis of aspergillosis, cryptococcosis, histoplasmosis, blastomycosis, paracoccidioidomycosis, and penicilliosis marneffei. The accuracy of the beta-glucan assay for diagnosis of aspergillosis and candidiasis and its role in fungal diagnosis remains unclear, in part because the few published studies report widely varying specificity. Serologic tests for antibodies are also useful for diagnosis of histoplasmosis and coccidioidomycosis, but their sensitivity may be reduced by immunosuppression. While molecular diagnostic methods have been described and are available at some reference and university laboratories, their role in patient care remains uncertain, largely because of the lack of well-characterized assays and studies establishing their accuracy. Culture methods, although essential for establishing the diagnosis in some cases, have limitations for rapid diagnosis, namely insensitivity, need for invasive procedures, and delayed growth.

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Section: Cryptococcosismentioning

confidence: 99%

Section: Penicilliosis Marneffeimentioning

confidence: 99%

Antigen detection, serology, and molecular diagnosis of invasive mycoses in the immunocompromised host

Wheat¹

2006

Transplant Infectious Dis

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“…Rapid, noninvasive microbiological diagnostic modalities for invasive fungal infections other than determinations of cryptococcal and histoplasma antigens are not available in most parts of the world (2,3,9). Although antigen detection to identify P. marneffei infection by using monoclonal antibodies specific for P. marneffei has been reported (2,3), these tests are not commercially available, which hampers their usefulness.…”

mentioning

confidence: 99%

Detection of Circulating Galactomannan in Serum Samples for Diagnosis ofPenicillium marneffeiInfection and Cryptococcosis among Patients Infected with Human Immunodeficiency Virus

et al. 2007

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“…When five sets of paired serumand urine specimens from patientswith penicilliosis were tested, all the serums amples were positive, as weref our of the fiveu rines. This group of investigators has alsod escribed the development and prospective evaluation of an antigen-capture ELISA in which two of their murine monoclonals, 8C3 and 8B11, were used (295).T he testw as evaluated using serums amples from 53 HIV-positive patients with penicilliosis, as well as 38 samples from HIV-positive patientsw ith candidiasis, cryptococcosis, or histoplasmosis, and 202s amples from control subjects. The ELISA had as ensitivity of 92%, as pecificity of 97.5%, aP PV of 89%, and aNPV of 98%.…”

Section: Antigen Detectionmentioning

confidence: 99%