Antifungal susceptibility of 1000 Candida bloodstream isolates to 5 antifungal drugs: results of a multicenter study conducted in São Paulo, Brazil, 1995–2003

Matta, Daniel Archimedes da; Almeida, Leila Paula de; Machado, Antônia Maria de Oliveira; Azevedo, Andréia Aparecida de; Kusano, Elisa Junko Ura; Travassos, Norma Fracalanza; Salomão, Reinaldo; Colombo, Arnaldo Lopes

doi:10.1016/j.diagmicrobio.2006.10.011

Cited by 71 publications

(60 citation statements)

References 31 publications

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“…In a multicenter study in São Paulo (Brazil), Da Matta et al (24) analyzed 1,000 isolates of candidemia from 1995 to 2003. In the first period, from 1995 to 1999, C. tropicalis was the second most frequent species (74/299).…”

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confidence: 99%

Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

et al. 2013

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fThe profiles of 61 Candida tropicalis isolates from 43 patients (28 adults and 15 children) diagnosed with candidemia at two teaching hospitals in São Paulo, Brazil, were characterized by multilocus sequence typing (MLST). For the 14 patients who had bloodstream infections, 32 isolates were serially collected from their blood and/or catheters. Thirty-nine diploid sequence types (DSTs) were differentiated. According to the C. tropicalis MLST database (http://pubmlst.org/ctropicalis/), 36 DSTs and 23 genotypes identified from the 61 isolates had not previously been described. This report represents the first study to characterize sequential isolates of C. tropicalis from candidemia cases in South America. Microvariation in a single gene was found in the sequential isolates from 7 patients. The main polymorphisms occurred in the alleles of the XYR1 gene, specifically at nucleotide positions 215, 242, and 344. Macrovariation in six gene fragments was detected in the isolates from 3 patients. eBURST analysis added two new groups to this study (groups 6 and 18). Additionally, susceptibility tests indicate that 3 isolates were resistant to fluconazole. No correlation was found between the DSTs and susceptibility to fluconazole and/or selective antifungal pressure. Two patients were sequentially infected with resistant and susceptible strains. MLST is an important tool for studying the genetic diversity of multiple/sequential isolates of patients with candidemia, allowing the comparison of our data with those from other regions of the world, as well as allowing an analysis of the genetic relationship among several clones in sequential isolates from the same or different candidemia patient sites (blood or catheter).

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confidence: 99%

Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

et al. 2013

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“…Em um trabalho realizado no Brasil com 400 C. albicans isoladas de sangue, todas se mostraram susceptíveis ao fluconazol e a anfotericina B (MATTA et al, 2007). Matta et al (2007), avaliando C. parapsilosis e C. tropicalis no Brasil, não encontrou resistência ao fluconazol nem a anfotericina B.…”

Section: Discussionunclassified

Comparação entre microdiluição e disco difusão para o teste de susceptibilidade aos antifúngicos contra Candida spp.

Júnior

Menezes

Cunha

et al. 2012

Semin. Cienc. Biol. Saude

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A evolução da resistência fúngica pode ser avaliada pelo teste de susceptibilidade aos antifúngicos. Os protocolos mais utilizados para testes de susceptibilidade são a micro diluição em caldo (MDC), disco difusão (DD) e E-test. O objetivo principal deste estudo foi comparar os testes de sensibilidade realizados pelos métodos de MDC e DD para a anfotericina B e o fluconazol contra cepas de Candida spp. isoladas em Fortaleza/CE. Foram utilizadas 40 C. albicans, 50 C. tropicalis e 47 C. parapsilosis, isoladas de amostras de sangue e urina de pacientes atendidos no Hospital Geral de Fortaleza. Os testes foram realizados pelos métodos MDC e DD, de acordo com os protocolos M27-A3 e M44-A2 do CLSI. As metodologias foram comparadas e calculadas os percentuais de concordância. Para a anfotericina B e fluconazol, ocorreu um predomínio de cepas com susceptibilidade. O percentual de concordância para as metodologias foi acima de 97% e não foram detectados erros graves. A utilização de DD para realização de testes de susceptibilidade antifúngica pode ser aplicada na rotina laboratorial, pois é uma técnica fácil, barata, e de confiança quando comparada ao teste de MDC, sem comprometer os resultados para as cepas de C. albicans, C. tropicalis e C. parapsilosis. Palavras-chave: Microdiluição. Disco difusão. Candida sp.

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“…In the city of São Paulo, a study of four tertiary hospitals by Matta et al analyzed all of the positive blood cultures for fungi from all of the patients admitted to these services (adults and children) in two different periods (1995-1999 and 2000-2003) and found that Candida albicans was higher in both periods, and in the second period there were more species of Candida. 33 In 2009, another study was also conducted in our country by Godim et al at a neonatal intensive care unit with ten beds at a Tertiary University Hospital, in the city of Uberlândia, regardless of the gestational age, found 19 cases of candidemia, with Candida albicans also being the most common, similar to all of the studies cited to date [nine cases of Candida albicans (47.9%), five of Candida krusei (26.3%), two of Candida parapsilosis (10.5%), two of Candida glabrata (10.5%), and one of Candida tropicalis (5.3%)]. 34 In Latin America, Nucci et al found 89 infants with candidemia, and 27% with C. parapsilosis.…”

Section: Mixed Syndromesmentioning

confidence: 99%

“…From the 1990s onwards, there was a significant increase in the number of infections caused by Candida non-albicans, such as Candida parapsilosis and other types of Candida in the global population. [30][31][32][33][34][35][36][37] Low T3-T4 syndrome described in critically ill adults and children is linked to poor prognosis and greater severity of the disease. In our study, all of the infants with fungal sepsis survived, so it is not possible to relate this type of syndrome with poor prognosis.…”

Section: Mixed Syndromesmentioning

confidence: 99%

Thyroid abnormalities in term infants with fungal sepsis

Silva¹,

Araújo²,

Diniz

et al. 2016

Rev. Assoc. Med. Bras.

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Objective: To describe thyroid alterations in term newborns (TNB) with fungal sepsis during NICU hospitalization. Method: The study included six TNB that during the clinical and laboratory manifestations of sepsis with positive cultures for fungus showed changes in thyroid hormones, called low T3 syndrome and low T3-T4 syndrome. TNB that could present hormonal changes caused by disease as those born to mothers with thyroid disease, or who had perinatal asphyxia and major surgeries were excluded. Results: Of six TNB with fungal sepsis, five had positive culture for Candida albicans and one had positive culture for Candida tropicalis. Low T3 syndrome was observed in two TNB (50%), while T3-T4 syndrome was observed in other two (100%). The four children progressed to septic shock. Conclusion: Fungal sepsis is becoming more common among newborns admitted to NICU. Thyroid insufficiency could be a marker of disease severity with possible need for hormone supplementation.

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Antifungal susceptibility of 1000 Candida bloodstream isolates to 5 antifungal drugs: results of a multicenter study conducted in São Paulo, Brazil, 1995–2003

Cited by 71 publications

References 31 publications

Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

Comparação entre microdiluição e disco difusão para o teste de susceptibilidade aos antifúngicos contra Candida spp.

Thyroid abnormalities in term infants with fungal sepsis

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