Antifungal Drug Susceptibility of Candida Species Isolated from HIV-Positive Patients Recruited at a Public Hospital in São Luís, Maranhão, Brazil

Terças, Ana L. G.; Marques, Sirlei Garcia; Moffa, Eduardo Buozi; Alves, Marcia Barros; Azevedo, Conceição M. P. S. de; Siqueira, Walter L.; Monteiro, Cristina de Andrade

doi:10.3389/fmicb.2017.00298

Cited by 41 publications

(38 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, MIC 50 values and MIC ranges generated from data collected during an exhaustive 12-year surveillance program in France (8) were very similar to those reported for the same species in the current report (Table 5). Moreover, a number of anecdotal case reports support our evidence for elevated MICs or fluconazole resistance in many of the species listed in Table 2, including Yarrowia (Candida) lipolytica (7,8), Pichia cactophila and Pichia norvegensis (7,8,(26)(27)(28)(29), C. blankii (30), Candida palmioleophila (7,31), Candida zeylanoides (29,32,33), Diutina (Candida) catenulata (7,34), Pichia fermentans (29), Pichia mandshurica (8), Debaryomyces hansenii (Candida famata) (29,35,36), Kodamaea ohmeri (8,35,(37)(38)(39), Trichosporon spp. (39)(40)(41), Rhodotorula spp.…”

Section: Discussionsupporting

confidence: 70%

Fluconazole Resistance in Isolates of Uncommon Pathogenic Yeast Species from the United Kingdom

Borman

Müller

Walsh-Quantick

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The triazole drug fluconazole remains one of the most commonly prescribed antifungal drugs, both for prophylaxis in high-risk patients and also as a second-line treatment option for invasive Candida infections. Established susceptibility profiles and clinical interpretive breakpoints are available for fluconazole with Candida albicans, Candida glabrata, Candida tropicalis, and Candida parapsilosis, which account for the majority of infections due to pathogenic yeast species. However, less common species for which only limited susceptibility data are available are increasingly reported in high-risk patients and from breakthrough infections. The UK National Mycology Reference Laboratory performs routine antifungal susceptibility testing of clinical isolates of pathogenic yeast submitted from across the United Kingdom. Between 2002 and 2016, ϳ32,000 isolates were referred, encompassing 94 different yeast species. Here, we present fluconazole antifungal susceptibility data generated using a CLSI methodology over this 15-year period for 82 species (2,004 isolates) of less common yeast and yeast-like fungi, and amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, and anidulafungin, with members of the Nakaseomyces clade (C. glabrata, Candida nivariensis, and Candida bracarensis). At least 22 different teleomorph genera, comprising 45 species, exhibited high MICs when tested with fluconazole (Ͼ20% of isolates with MICs higher than the clinical breakpoint [Ն8 mg/liter] proposed for C. albicans). Since several of these species have been reported anecdotally from breakthrough infections and therapeutic failures in patients receiving fluconazole, the current study underscores the importance of rapid and accurate yeast identification and may aid clinicians dealing with infections with rarer yeasts to decide whether fluconazole would be appropriate.

show abstract

Section: Discussionsupporting

confidence: 70%

Fluconazole Resistance in Isolates of Uncommon Pathogenic Yeast Species from the United Kingdom

Borman

Müller

Walsh-Quantick

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…The modern mycologist has an important role to play in several aspects relating to these organisms, including detection, identification, epidemiological analysis, and therapy in an attempt to better understand these pathogens and provide an effective cure (Maheshwari et al, 2016). This could guide the therapeutic choice and the clinical treatment (Terças et al, 2017). The overall prevalence of urinary candidiasis among HIV infected patients was found to be 18.8% and 36.2% in two localities of Cameroon, Buea and Yaounde respectively (Lohoué et al, 2005;Longdoh et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Urinary tract candidiasis in HIV+ patients and sensitivity patterns of recovered<i> Candida </i>species to antifungal drugs in Dschang District Hospital (Cameroon)

Diesse¹,

Kechia²,

Iwewe³

et al. 2017

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

The incidence of Candida urinary tract infections is gradually on the rise and is an important public health problem. The aim of our study was to determine the prevalence of urinary tract candidiasis (candiduria) in HIV positive patients in Dschang District Hospital and the antifungal susceptibility test of isolates. A total of 285 patients were recruited for this study. Midstream urine samples were collected and processed using standard mycological techniques. Candida isolates were identified base on the colony color on CHROMagar. Antifungal susceptibility testing of the isolates was performed by the broth dilution method using four commonly used antifungals. Results showed that 22% of patients had Candida spp in their urine. Candida albicans had a proportion of 37% against 63% for non-albicans Candida. Of the 53 isolates tested, ketoconazole had the highest percentage of resistance (88.6%) follow by fluconazole (64.1.6%), amphotericin B (56.6%) and nystatin (49.0%). The highest sensitivity was observed with nystatin (33.9%) while the lowest was found with ketoconazole (5.6%). In conclusion, the prevalence of candiduria among HIV positive patients in this study was 22%. Candida albicans remain the most frequently involved Candida species. Azole antifungals showed the highest resistance rate against all the Candida species isolates.

show abstract

“…Several studies have recently investigated the fluconazole susceptibility of Candida isolates from HIV, cancer, and immunocompromised patients in Brazil 47,48 . However, no other studies have investigated the fluconazole susceptibility of Candida species associated with diabetic and kidney transplant patients.…”

Section: Discussionmentioning

confidence: 99%

ERG11 gene polymorphisms and susceptibility to fluconazole in Candida isolates from diabetic and kidney transplant patients

Benedetti

Savi

Aluízio

et al. 2019

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Candidiasis is the most frequent opportunistic mycosis in humans and can cause mortality, particularly in immunodeficient patients. One major concern is the increasing number of infections caused by drug-resistant Candidas trains, as these cannot be efficiently treated with standard therapeutics. The most common mechanism of fluconazole resistance in Candida is mutation of ERG11, a gene involved in the biosynthesis of ergosterol, a compound essential for cell integrity and membrane function. Methods: Based on this knowledge, we investigated polymorphisms in the ERG11 gene of 3 Candida species isolated from immunocompromised and immunocompetent patients. In addition, we correlated the genetic data with the fluconazole susceptibility profile of the Candida isolates. Results: A total of 80 Candida albicans, 8 Candida tropicalis and 6 Candida glabrata isolates were obtained from the saliva of diabetic, kidney transplant and immunocompetent patients. Isolates were considered susceptible to fluconazole if the minimum inhibitory concentration was lower than 8 μg/mL. The amino acid mutations F105L, D116E, K119N, S137L, and K128T were observed in C. albicans isolates, and T224C and G263A were found in C. tropicalis isolates. Conclusions: Despite the high number of polymorphisms observed, the mutations occurred in regions that are not predicted to interfere with ergosterol synthesis, and therefore are not related to fluconazole resistance.

show abstract

Antifungal Drug Susceptibility of Candida Species Isolated from HIV-Positive Patients Recruited at a Public Hospital in São Luís, Maranhão, Brazil

Cited by 41 publications

References 43 publications

Fluconazole Resistance in Isolates of Uncommon Pathogenic Yeast Species from the United Kingdom

Fluconazole Resistance in Isolates of Uncommon Pathogenic Yeast Species from the United Kingdom

Urinary tract candidiasis in HIV+ patients and sensitivity patterns of recovered<i> Candida </i>species to antifungal drugs in Dschang District Hospital (Cameroon)

ERG11 gene polymorphisms and susceptibility to fluconazole in Candida isolates from diabetic and kidney transplant patients

Contact Info

Product

Resources

About