Antifungal activity of silver nanoparticles and clotrimazole against Candida spp.

Abstract

The aim of present study was calculate the Minimum inhibitory concentrations (MICs) of silver nanoparticles and clotrimazole for Candida species and their interaction by the adaptation of standarized methods. The MICs values of clotrimazole were 9 E -04 -3 E -03 ug/ml, 0.1-0.6 ug/ml, 3 E -03 -0.1 ug/ml and 3 E -03 -0.3 ug/ml for Candida albicans susceptible to fluconazole, Candida albicans resistance to fluconazole, Candida krusei and Candida parapsilosis respectively. The MICs values of silver nanoparticles were 26.50-53 ug/ml; 26.50-106 ug/ml; 106-212 ug/ ml and 26.50-53 ug/ml for Candida albicans susceptible to fluconazole, Candida albicans resistance to fluconazole, Candida krusei and Candida parapsilosis respectively. Synergism between clotrimazole and silver nanoparticles was measured by checkerboard BMD (broth microdilution) test and shown only for C. albicans susceptible to fluconazole because the fractional inhibitory concentrations (FICs) values were 0.07 -0.15 ug/ml. Indifference was shown for the other species tested because the FICs values were between 0.5 -2-3.06 ug/ml. The results suggest synergistic activity depending on the fungus species analysed, however we recommend the incorporation of others measurement methodologies to confirm our results. As for measurement methodologies of MICs of silver nanoparticles and clotrimazole international normative were respected to guarantee reproducible and comparable results.

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“…These important changes in the membrane are acknowledged by the formation of “pits” on its surface and eventually provide for the formation of pores and cell death. 190 Fluconazole in combination with AgNPs showed the maximum suppression of C. albicans , followed by P. glomerata and Trichoderma sp. 191 Tineapedis (a common skin infection) and nail mycosis (a nail infection) are very widespread in adults, induced by the skin disease mold Trichophyton .…”

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