Antifungal activity of Rubia tinctorum, Rhamnus frangula and Caloplaca cerina

Manojlović, N.; Solujić, Slavica; Sukdolak, Slobodan; Milosev, M.

doi:10.1016/j.fitote.2004.12.002

Cited by 81 publications

(44 citation statements)

References 5 publications

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“…It is native to northernmost Africa Europe and western Asia (Hänsel and Sticher 2007). Previous studies have demonstrated that crude extract of R. frangula has strong antioxidant, antimicrobial, and free radical scavenging activities (Manojlovic et al 2005;Ş tef et al 2009). Although R. frangula has been known as a potential source of various bioactive molecules and one of the most common laxatives, it was not described as a protease inhibitor source so far.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

et al. 2017

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Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was selected as a protease inhibitor source. The maximum recovery of the protease inhibitor against trypsin was recorded in the crude extract made in 0.1 M phosphate buffer (pH 7.0) and isolated from the mature leaves. Then, the protease inhibitor designated as RfIP1 was purified to homogeneity by Sephadex G50 with an apparent molecular mass of 22.5 kDa and its N-terminal sequence exhibited a high degree of homology with known serine protease inhibitor sequences. The RfIP1 displayed maximal activity at pH 7 and 37°C. It maintained almost 80% of its maximal activity through a large pH range. The thermo-stability of RfIP1 was markedly enhanced by BSA, CaCl 2, and sorbitol, whereas the addition of Mg 2? , Zn 2? , NaTDC, SDS, DTT, and b-ME significantly promoted inhibitory activity. The protease inhibitor displayed high inhibitory activity toward some known proteases (cathepsin B, chymotrypsin, collagenase, thrombin, and trypsin) that have more importance in pharmaceutical industry and it acted as potent inhibitor of some commercially proteases from Aspergillus oryzae, Bacillus sp, and Bacillus licheniformis. The protease inhibitor also possessed an appreciable antibacterial effect against both Gram-positive and Gram-negative bacteria.

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Section: Introductionmentioning

confidence: 99%

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

et al. 2017

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“…In Morocco Rubia tinctorum L. roots have been used as antidiarrheic, it has been also used in blood diseases, and reputed as aphrodisiac (Bellakhdar, 1997). The plant is reported to possess antimicrobial (Kalyoncu et al, 2006), antifungal activities (Manojlovic et al, 2005) and endowed with diuretic and stone inhibitory activities (Wijnsma et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Anti-diarrhoeal activity of crude aqueous extract of Rubia tinctorum L. roots in rodents

Karim¹,

Mekhfi²,

Ziyyat³

et al. 2010

J. Smooth Muscle Res.

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The Anti-diarrhoeal effect of aqueous extract of Rubia tinctorum L. (Rubiaceae) roots in rodents was examined. At doses 300, 600 and 800 mg/kg aqueous extract protected rats, in a dose-dependent fashion, against castor oil-induced diarrhoeal dropping by 37, 59 and 64% respectively. Furthermore, it has significantly inhibited by 41% the gastrointestinal transit of charcoal in mice at 800 mg/kg dose of extract. These data suggest that Rubia tinctorum showed antidiarrhoeal activity by inhibiting intestinal motility which was concordant with its use in traditional medicine.

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“…Some lichen substances such as usnic and haematommic acids inhibit the growth of moulds (Nash 1996). Antifungal activity in particular for the anthraquinone parietin has been reported by Manojlovic et al (2005). Aqueous and acetone extracts of lichens Ramalina spp.…”

Section: Provision Of Lichen Secondary Metabolites and Other Compoundsmentioning

confidence: 95%

The Diversity of Lichenised Fungi: Ecosystem Functions and Ecosystem Services

Zedda¹,

Rambold

2015

Recent Advances in Lichenology

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Antifungal activity of Rubia tinctorum, Rhamnus frangula and Caloplaca cerina

Cited by 81 publications

References 5 publications

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

Anti-diarrhoeal activity of crude aqueous extract of Rubia tinctorum L. roots in rodents

The Diversity of Lichenised Fungi: Ecosystem Functions and Ecosystem Services

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