Antifungal Activity of Nine Medicinal Plants against Aspergillus species from Cocoa Beans (Theobroma cacao)

Orole, Olukayode Olugbenga; Adejumo, T. O.; Orole, R.

doi:10.9734/jaeri/2016/24602

Cited by 11 publications

(9 citation statements)

References 14 publications

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“…The results of this study are in line with the results of a study by [21] suggesting that ginger extract was able to inhibit the growth of Aspergillus foetidus, Aspergillus versicolor, Aspergillus carbonarius, and Aspergillus nidulan at 31, 59, 64, and 73 % when compared to controls. Ginger extract with a concentration of 15 % can inhibit the growth of mycelia Alternaria mold by 57 % when compared to controls [22].…”

Section: Extract Concentration Colony Diameter (Mm) Percentage Of Inh...supporting

confidence: 91%

The Ability of Seasoning to Inhibit Contaminant Aspergillus spp. on Traditional Food Sardine Pedetan (Sardinella lemuru)

et al. 2022

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Pedetan is processed food of seasoned sardine fish (Sardinella lemuru) which is preserved by drying. Isolation and identification of mold that contaminated the pedetan have been taken from 10 villages which are production centers in Jembrana Regency. Molds that can contaminate pedetan during storage, include Aspergillus flavus, Aspergillus aculeatus, Aspergillus niger, and Aspergillus tubingensis. In this research, the composition of the seasoning from the producer was used to inhibit the growth of contaminant fungi. The minimum antimicrobial inhibition test was carried out by the well diffusion method and the seasoning extract was tested for its inhibition on mold growth on potato dextrose agar (PDA) media. Mold colonies growth test and testing the effect of extracts on mold biomass were carried out in vitro with potato dextrose broth (PDB) media. The results showed that the composition of seasoning garlic, coriander, kaemfiera, galangal, ginger, vinegar, and salt can inhibit Aspergillus spp. Seasoning extract concentrations of 0.5 % (w/v) diameter of inhibitory zone minimum inhibitory concentration (MIC) are 0.8 and 1.2 mm for A. aculeatus and A. niger, by 1 mm for A. flavus and A. tubingensis, respectively. Seasoning extract at a concentration of 0.5 % (w/v) is also able to inhibit up to 100 % to the growth of the colony, conidia germination, conidia density, and biomass of A. flavus, A. aculeatus, A. niger, and A. tubingensis that contaminate the sardine fishes pedetan. HIGHLIGHTS Pedetan is processed food of seasoned sardine fish (Sardinella lemuru) which is preserved by drying Molds that can contaminate pedetan during storage, include Aspergillus flavus, Aspergillus aculeatus, Aspergillus niger, and Aspergillus tubingensis The composition of seasoning garlic, coriander, kaemfiera, galangal, ginger, vinegar, and salt can inhibit Aspergillus spp GRAPHICAL ABSTRACT

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Section: Extract Concentration Colony Diameter (Mm) Percentage Of Inh...supporting

confidence: 91%

The Ability of Seasoning to Inhibit Contaminant Aspergillus spp. on Traditional Food Sardine Pedetan (Sardinella lemuru)

et al. 2022

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“…Barra-Bucarei et al [35] suggested that the action of B. bassiana as an endophyte increases the plant's ability to resist the attack of pathogens such as B. cinerea and Fusarium ssp [36]. In tomatoes, chili peppers and maize plants, B. bassiana acts as an opportunistic parasite on various phytopathogens, as it has pathogenic and saprophytic habits.…”

Section: Discussionmentioning

confidence: 99%

Effects of Plant Extracts and Beauveria bassiana on the Activity of Defense-Related Enzymes in Solanum lycopersicum L. during Interaction with Fusarium oxysporum f. sp. lycopersici

Perez-Robles,

Alberto Lecona-Guzm醤,

Manuel Ru韟-Valdiviezo

et al. 2023

Phyton

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The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum L. infected with Fusarium oxysporum after the combined application of Beauveria bassiana and plant extracts. Solanum lycopersicum plantlets were transplanted 15 days after the emergency. Five days after transplanting, Beauveria bassiana spores were applied at a concentration of 1 × 10 7 spores mL −1 onto soil (along with A. indica (N) and P. auritum (H) leaf extracts) where S. lycopersicum plants were planted. Eight days after transplanting, spores of F. oxysporum strain were applied at a concentration of 1 × 10 6 spores mL −1 to soil where S. lycopersicum plants were growing. The development of S. lycopersicum plants was monitored for 114 days, whereby a randomized complete block treatment design was used, the roots of the plants were crushed with liquid nitrogen, after which UV/VIS spectrophotometry was used to determine protein concentration, as well as the activity of β-1,3glucanases, peroxidases (POX, EC1.11.1.7), catalase and chitinases. The treatments combining B. bassiana and A. indica and P. auritum extracts, had a significant difference in enzymatic activity levels, thus contributing to better defense mechanisms and a greater protection to S. lycopersicum plants in the presence of F. oxysporum. The application of B. bassiana and plant extracts to the ground mitigated damage caused by F. oxysporum on S. lycopersicum plants.

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“…Radial growth of pathogenic fungi in the presence or absence of the endophyte was measured after 7 days, and antagonistic percentage (A) was calculated using the following formula: A (%) = CDC-CDT / CDCx100, where CDC represents the colony diameter growth (mm) of the control plate and CDT represents the colony growth diameter of pathogen on the test plate (Abdennabi et al, 2017). According to Nuangmek et al (2008) and Orole and Adejumo (2009), if the antagonistic percentage is: a) less than 30%, the antifungal activity of the endophytes is low; b) between 30 and 50%, the antifungal activity is moderate; c) between 50 and 70%, the activity is high and d) above 70%, the activity is very high.…”

Section: Antagonistic Activity Of Endophytesmentioning

confidence: 99%

“…Endophytes modulate the plant's immune system and increase the production of secondary metabolites by its host plant (Khare et al, 2018). Some of the endophytes like Trichoderma koningii and Alternaria alternata from maize roots have antagonistic effects on Fusarium pathogen (Orole and Adejumo, 2009). Banana endophytes inhibited the growth of Fusarium oxysporum f. sp cubense and Colletotrichum guaranicola (Souza et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Bioactivity of Some Endophytic Fungi from Cameroonian Medicinal Plants against Verticillium albo-atrum, Rhizoctonia carotae and Phytophthora megakarya

Djeugap,

Mbatkam,

Kouam

et al. 2024

Int. J. Phytopathol.

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Endophytic fungi (EFs) are beneficial microorganisms which grow in living plant tissues without causing any disease to their hosts. Most of them have antifungal properties. EFs of three herbaceous plants namely Lantana camara, Emilia coccinae and Bryophyllum pinnatum were isolated by using potato dextrose agar medium (PDA) and screened for their antifungal activity on the double direct confrontation (DDC) test on PDA. The growth diameter of pathogenic fungi with or without endophyte was measured weekly. Then, fourteen days after the DDC test, fungicidal or fungistatic activity of the EFs was assessed on the pathogens’ growth. Finally, EFs that strongly inhibited pathogens’ growth were submitted to the morphological characterization on PDA, Malt Extract Agar (MEA) and Sabouraud Dextrose Agar (SDA) media at three pH levels (5.2, 7 and 9). Results showed that eight EFs genera are associated with these herbaceous medicinal plants with diverse antifungal activities. EFs that significantly (p 0.05) inhibited the growth of the three pathogenic fungi were: Trichoderma harzianum (66%), Cercospora sp (58%) and Aspergillus sp (49%), 7 days after the DDC test. Endophytes T. harzianum and Cercospora sp were fungicidal on the three pathogens while Aspergillus sp was fungicidal on V. albo-atrum and R. carotae. There was a high morphological variability (colour, texture and pigments produced by EFs on culture media), and variability in their daily growth diameter and sporulation among EFs from one medium to another and from one pH level to another. This study suggests that T. harzianum and Cercospora sp endophytes possess the high antagonistic activity and can be used as an alternative to synthetic chemicals that control plant diseases

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Antifungal Activity of Nine Medicinal Plants against Aspergillus species from Cocoa Beans (Theobroma cacao)

Cited by 11 publications

References 14 publications

The Ability of Seasoning to Inhibit Contaminant Aspergillus spp. on Traditional Food Sardine Pedetan (Sardinella lemuru)

The Ability of Seasoning to Inhibit Contaminant Aspergillus spp. on Traditional Food Sardine Pedetan (Sardinella lemuru)

Effects of Plant Extracts and Beauveria bassiana on the Activity of Defense-Related Enzymes in Solanum lycopersicum L. during Interaction with Fusarium oxysporum f. sp. lycopersici

Bioactivity of Some Endophytic Fungi from Cameroonian Medicinal Plants against Verticillium albo-atrum, Rhizoctonia carotae and Phytophthora megakarya

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