Antifungal Activity of Human and Some Mammals Sera

Abstract

Aim. Estimation of activity of native human serum and its antimicrobial peptides fraction against clinically important yeasts and comparison with the activity of some mammals sera. Materials and methods. Pooled samples of human, bovine, rabbit and mouse sera and collection strains of yeasts Candida albicans, Rhodotorula mucilaginosa, Malassezia furfur, Cryptococcus neoformans, Geotrichum candidum, Trichosporon cutaneum, Saccharomyces cerevisiae were used in the study. Antimicrobial peptides fractions (AMP) were obtained by filtration through molecular filters with 100 kDa pores. Activity of sera and their AMP-fractions were estimated by spectrophotometric method. Results. Activity of native mammal sera varied in diapason 73÷89% independently from yeast genus, although AMP-fractions activity varied more significantly. The minimal sensitivity to AMP-fractions of sera demonstrated M. furfur (activity values were equal 0÷13,5%) and G. candidum (0÷6,5%), but the maximal — R. mucilaginosa (12,3÷56,4%), C. albicans (22,0÷32,9%), and C. neoformans (17,1÷29,9%). Activity values of AMP-fractions of human serum were correlated meaningfully with no of the values of other mammals (Pirson coefficient r=0,459÷0,527). Considerable correlation of the indexes took place between rabbit and bovine sera (r=0,827), as well as between rabbit and mouse sera (r = 0,753). Conclusion. The differences between AMP-fractions activity towards studied yeast genera/specia indicate the occurrence of its specificity probably related with structural organization of cytoplasmic membrane of yeast cells as well as with variations in AMP composition in different mammals.

Effect of Rabbit Immunization with Yeast Antigens on the Activity of the Fraction of Serum Antimicrobial Peptides

“…Данный способ заключается в действии образцов сыворотки на клетки тест-культур микроорганизмов и спектрофотометрическом измерении процента красителя, вошедшего в убитые клетки, по сравнению с контролем. С помощью этого метода определены указанные свойства сывороток 4 видов млекопитающих, включая человека, и обнаружены различия в величинах противогрибковой активности (ПГА) АМП-фракций сывороток, но не в величинах общей активности [3]. Этим же методом показано, что иммунизация мышей дрожжевыми антигенами приводила не только к увеличению уровней специфических иммуноглобулинов класса G, но и к повышению активности фракции АМП по отношению к Candida albicans [4].…”