Yeast microflora was studied in the skin of 91 patients with atopic dermatitis, in bronchial secretion of 13 patients with bronchial asthma and 8 patients with allergic bronchopulmonary mycosis. Forty-eight isolates were obtained. Alypophilic yeast fungi isolated from the skin were presented mainly by genera Candida spp. (48%) and Rhodotorula spp. (29%), while the cultures isolated from bronchial secretion mainly by Candida albicans. The sensitivity of yeast cultures to the antifungal drugs diflucan, clotrimazole, nizoral, orungal, exoderil, levorin, pymafuzin, and nitrofungin was determined. The most efficient drugs were diflucan, clotrimazole, nizoral, and orungal. More than half isolates were sensitive only to the high concentrations of levorin (48%), pymafuzin (75%), and nitrofungin (82%); 64% isolates were insensitive even to high concentrations of exoderil. Preliminary in vitro selection of the antifungal drugs is required for efficient elimination of the yeasts.

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Except immunoglobulins and components of complement system, the important part of congenital humoral immunity are antimicrobial (poly) peptides (AMP). Main antimicrobial mechanism of AMP action is the destruction of cytoplasmic membrane. In host organism number of AMP act simultaneously, therefore method for the measuring of overall AMP activity would be helpful. The method was recently applied and modified for estimation of AMP activity in serum: aliquots of low-molecular fraction of serum added to test-culture of Candida albicans, and the part of killed cells, i.e. total activity of AMP, calculated by spectroscopy. Aim of present study is the estimation of AMP activity in mice sera after immunization with cells of clinical yeasts compare to the immunoglobulins G.Yeasts strains-Candida albicans, Cryptococcus neoformans, Rhodotorula mucilaginosa, Trichosporon cutaneum, Geotrichum candidum, and Saccharomyces cerevisiae-were cultivated in liquid defined media up to the end of exponential growth phase. Obtained yeasts cells used for immunization of mice, but yeast antigens-for the estimation of IgG-antibodies levels in mice sera by ELISA method. AMP activity were measured as described above.Immunization of mice with yeasts cells was accompanied not only with increase of specific IgG levels, but with elevation of total activity of AMP: for example specific IgG in sera of mice immunized by C. albicans cells was 16-fold higher than in non-immunized (control), and AMP activity-1,5 fold higher than in control. Correlation between specific humoral response at the immunoglobulins level and humoral response at the AMP-activity level (Pirson`s coefficient r=0,735; p ≤ 0,05) as a result of immunization of mice by fungal cells was demonstrated for the first time. The method may be used for study of humoral immunity associated with action of AMP in various animal and human models.