Antifungal activity of eugenol against Botrytis cinerea

Abstract

The antifungal properties of eugenol were tested against ten plant pathogenic fungal species and detailed studies were conducted regarding in vitro activity of eugenol on Botrytis cinerea. The EC 50 value of eugenol on mycelial radial growth of B. cinerea was 38.6 µg/ mL; however, eugenol had no bioactivity against conidia germination. B. cinerea hyphae treated with eugenol showed strong propidium iodide fluorescence in the cytosol. Eugenol increased the concentration of potassium ion and cellular materials in the medium. Furthermore, light and scanning electron microscopy observations on hyphae exposed to eugenol revealed considerable morphological alterations in hyphae, such as cytoplasmic coagulation, vacuolation, and hyphal shriveling. Eugenol induced the generation of H 2 O 2 and increased free Ca 2+ in the cytoplasm. These results strongly support the idea that the antifungal activity of eugenol is due to membrane binding and permeability alteration, leading to destabilization and disruption of the plasma membrane.

Inhibitory effect of nerol against Aspergillus niger on grapes through a membrane lesion mechanism

“…The results demonstrated that NEL has pronounced antifungal activity against A. niger in vitro. Similar results were reported by Wang, Zhang, Chen, Fan, and Shi (2010), who found that the EC 50 value of eugenol on mycelial radial growth in Botrytis cinerea was 38.6 mg/mL, but eugenol had no bioactivity against spore germination. Some investigators have indicated that the lipophilic nature of essential oils allows them to easily pass through cell membranes to induce biological responses.…”

Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

“…10 Effectiveness of EON in controlling Fusarium wilt under greenhouse conditions. a Cotton seedlings treated with eugenol oil nanoemulsion b, Severely affected seedlings after planting in artificially infested soil in a greenhouse test (untreated cottonseed) (Vein discoloration began at the margin of cotyledonary leaf) a Relative contribution (RC) to variation in radicle lengths calculated as percentage of sum of square of the explained (model) variation of food-borne (Ghosh et al 2014), of plant pathogens (Wang et al 2010), and mycotoxigenic fungi (Morcia et al 2012). On the other hand, little attention has been paid to its antifungal activity against Fusarium wilt in cotton.…”

“…on media. Eugenol, a main component of clove oil, was proposed as the agent responsible for clove oil's antifungal activity against a wide range of plant pathogens (Pinto et al 2009;Wang et al 2010;Matan et al 2011;Ghosh et al 2014). Therefore, the present study was aimed to formulate and characterize the eugenol oil nanoemulsion using non-ionic surfactant Tween 20 with water as a continuous aqueous phase.…”

Efecto de aceites naturales contra Micosphaerella fijiensis en condiciones in vitro y detección de fitoquímicos activos

“…Ante estas evidencias, se Barrera and García (2008) reported that cinnamic aldehyde and carvacrol inhibited completely Fusarium spp fungal mycelial growth at 100 µg mL -1 . Wang et al (2010) …”