Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

Abd-Elsalam, Kamel A.; Khokhlov, Alexei R.

doi:10.1007/s13204-014-0398-y

Cited by 118 publications

(44 citation statements)

References 34 publications

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“…EG nanoemulsion was prepared by using high‐energy method described previously (Abd‐Elsalam & Khokhlov, ; Bhargava, Conti, da Rocha, & Zhang, ; Ghosh et al, ). As in emulsion preparation, nanoemulsion with different SORs were prepared and were evaluated for the stability.…”

Section: Methodsmentioning

confidence: 99%

“…The measurements were taken from three replicates of stock solutions. The stability of EG emulsion was determined using a standard published method (Abd-Elsalam & Khokhlov, 2015;Shafiq & Shakeel, 2010). Briefly, formulations of EG were centrifuged at 2,537g for 20 min and evaluated for phase separation.…”

Section: Characterization Of Emulsionmentioning

confidence: 99%

“…2.4 | Study 3: Evaluation of efficacy of EG nanoemulsion as an antimicrobial wash treatment on chicken skin 2.4.1 | Preparation and characterization of nanoemulsion EG nanoemulsion was prepared by using high-energy method described previously (Abd-Elsalam & Khokhlov, 2015;Bhargava, Conti, da Rocha, & Zhang, 2015;Ghosh et al, 2013). As in emulsion preparation, nanoemulsion with different SORs were prepared and were evaluated for the stability.…”

Section: Preparation Inoculation and Treatment Of Chicken Skin Samentioning

confidence: 99%

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Eugenol as an antimicrobial wash treatment reduces Campylobacter jejuni in postharvest poultry

Wagle

Arsi

Shrestha

et al. 2019

Journal of Food Safety

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The efficacy of the natural plant‐derived compound, eugenol (EG), as an antimicrobial wash treatment to reduce Campylobacter jejuni in postharvest poultry was investigated. The antimicrobial efficacy of EG was studied as a suspension, emulsion, or nanoemulsion treatment (two trials each). In each trial, chicken skin samples were inoculated with C. jejuni (∼7.2 Log CFU/sample), washed with treatments (0, 0.125, 0.25, 0.5, 1, or 2% EG corresponds to 0, 7.61, 15.22, 30.45, 60.90, or 121.8 mM, respectively) for 1 min, drip dried for 2 min, and then processed at 0, 8, and 24 hr of refrigerated storage (n = 5 samples/treatment/time point). All doses of the EG suspension consistently reduced C. jejuni counts with the greatest reduction (>2.0 Log CFU/sample) for the 2% dose when compared with controls (p < .05). EG emulsions or nanoemulsions did not provide any additional reduction in C. jejuni when compared to EG suspension. Our results suggest that EG could be an effective postharvest intervention strategy for reducing C. jejuni contamination on poultry products. Practical Applications Campylobacter jejuni, a leading cause of foodborne illness in humans, is strongly associated with the consumption of contaminated poultry products. Interventions reducing C. jejuni contamination in poultry would reduce the risk of subsequent human infections. In this study, the antimicrobial efficacy of eugenol was studied in three different delivery systems; suspension, emulsion, or nanoemulsion. Our results demonstrated that eugenol was effective in reducing C. jejuni counts on chicken skin and can be used as a potential strategy to reduce Campylobacter on poultry products.

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Section: Methodsmentioning

confidence: 99%

Section: Characterization Of Emulsionmentioning

confidence: 99%

Section: Preparation Inoculation and Treatment Of Chicken Skin Samentioning

confidence: 99%

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Eugenol as an antimicrobial wash treatment reduces Campylobacter jejuni in postharvest poultry

Wagle

Arsi

Shrestha

et al. 2019

Journal of Food Safety

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“…The samples were observed by operating at 160 kV. The average particle size was calculated from TEM images using the ImageJ software 19 .…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms)mentioning

confidence: 99%

Effect of nanoencapsulation on volatile constituents, and antioxidant and anticancer activities of Algerian Origanum glandulosum Desf. essential oil

Ali

Al-Khalifa

Aouf

et al. 2020

Sci Rep

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Nanoencapsulation is an attractive novel technique used for incorporating essential oils in food preparations and pharmaceutical formulae. This study investigated the effect of nanoencapsulation on the composition of volatile compounds, as well as the antioxidant and anticancer activities of hydrodistilled (HD) Origanum glandulosum Desf. Oil, which was encapsulated into nanocapsules via High Speed Homogenization (HSH) and into nanoemulsions through High Pressure Homogenization (HPH). Thirty-two volatile components were identified using Gas Chromatography-Mass Spectrometry analysis (GC-MS) in HD essential oil representing 99.04% of the total oil content. GC-MS analysis showed that the use of HPH to prepare nanoemulsions negatively affected the active compounds present in HD oil, particularly carvacrol and thymol, whereas the use of HSH led to significant quantitative differences in the composition of volatiles between HD oil and nanocapsules but generated the same profile. Consistent with the differences in total phenolics, total flavonoids, and volatiles identified in HD and nanoparticles, HD essential oil exhibited a higher antioxidant activity (IC 50 4.22 mg/ mL) than nanocapsules (IC 50 57.51 mg/mL) and nanoemulsion (IC 50 78.50 mg/mL), while nanocapsules showed the strongest cytotoxic effect on liver cancer cell line Hep-G2 (54.93 μg/mL) in comparison to HD oil (73.13 μg/mL) and nanoemulsions (131.6 μg/mL).The Origanum genus (Lamiaceae family) includes approximately 38 species that have been studied extensively for potential importance, and uses in flavoring foods and traditional medicine due to their pharmacological characteristics 1 . Origanum species are widely distributed in North Africa, eastern Mediterranean, and Siberian regions. According to Kokkini 2 , Origanum taxa are rich in essential oils that exhibit well-known antioxidant and antimicrobial properties. Based on the chemical composition of essential oils, Origanum species have been classified into three main chemotypes: thymol/carvacrol, linalool/terpinen-4-ol, and sesquiterpenes.Origanum glandulosum Desf., which belong to thymol and /or carvacrol chemotype, is an endemic herb of Algeria, Morocco and Tunisia used in traditional medicine to treat cough, rheumatism, diabetes, and fever 3 . Previous studies have reported antioxidant, antimicrobial, antifungal, and insecticidal activities of the essential oil extracted from O. glandulosum Desf 4-7 . However, to the best of our knowledge, the anticancer properties of this oil have not yet been investigated, despite the focus of several recent studies on the use of natural products with potent antioxidant activity in cancer treatment 8 .Like most essential oils, the use of O. glandulosum Desf. oil in food or pharmaceutical industries may have some limitations owing to its aroma, flavor, volatility, poor dispersibility in hydrophilic media, and sensitivity

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“…Nevertheless, the same problems with their usage exist. To enhance antifungal activity and decrease environmental toxic effect, it is important to develop novel type of biocides [1,2].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of chitosan–copper nanocomposites and their fungicidal activity against two sclerotia-forming plant pathogenic fungi

et al. 2017

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In this report, the metal-vapor synthesis (MVS) was used for the preparation of copper nanoparticles which was then used for the preparation of chitosan-copper nanocomposite. The antifungal activity of Cu@Chit NCs against two sclerotium-forming plant pathogenic fungi Sclerotium rolfsii (S. rolfsii) and Rhizoctonia solani (R. solani) AG-4 was evaluated in vitro and their effects on hyphal morphology, and sclerotia formation were observed for the first time. The NCs were prepared through impregnation of chitosan with colloid solution of copper nanoparticles in organic solvent (acetone or toluene). Transmission electron microscopy shows that the particles have predominantly spherical form, polydisperse character, the mean diameter about 2-3 nm and a rather uniform distribution in the chitosan matrice. Analysis of the small angle scattering curves suggests that the copper particles in the NCs with the size of B2 nm are mostly located in the chitosan pores with the same size. The effect of Cu@Chit NCs on fungal growth reveals some significant inhibitory activity against two tested fungi. The highest level of inhibition against S. rolfsii and R. solani AG-4 was observed using the high concentrations of Cu@Chit NC prepared using acetone as a solvent. A loss of the cytoplasm content, cytoplasmic coagulation, irregular shape of mycelia, or destruction in the hyphae was confirmed. The experiments demonstrate that the Cu@Chit NC synthesized via MVS using acetone was more effective than that of toluene in inhibiting fungal hyphae growth against R. solani AG-4 and S. rolfsii. The results show that the Cu@Chit NCs are fungicidal against both the tested fungus at high concentrations and the fungicidal or fungistatic activity is dependent on the tested fungus species.

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Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

Cited by 118 publications

References 34 publications

Eugenol as an antimicrobial wash treatment reduces Campylobacter jejuni in postharvest poultry

Eugenol as an antimicrobial wash treatment reduces Campylobacter jejuni in postharvest poultry

Effect of nanoencapsulation on volatile constituents, and antioxidant and anticancer activities of Algerian Origanum glandulosum Desf. essential oil

Synthesis and characterization of chitosan–copper nanocomposites and their fungicidal activity against two sclerotia-forming plant pathogenic fungi

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